Q8G2Q0 (URE31_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Urease subunit gamma 1
EC=3.5.1.5

Alternative name(s):
Urea amidohydrolase subunit gamma 1

Gene names

Name:	ureA1
Synonyms:	ureA-1
Ordered Locus Names:	BR0268, BS1330_I0269

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	100 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Disrupting the ure1 operon causes loss of urease activity, decreased resistance to low pH killing in vitro and decreased pathogen survival when innoculated in BALB/c mice by gavage. Ref.3
Catalytic activity	Urea + H2O = CO2 + 2 NH3. HAMAP MF_00739
Pathway	Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1. HAMAP MF_00739
Subunit structure	Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00739.
Sequence similarities	Belongs to the urease gamma subunit family.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW urea metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel cation binding Inferred from electronic annotation. Source: InterPro urease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 100	100	Urease subunit gamma 1 HAMAP MF_00739		PRO_0000098003

Sequences

	Sequence		Length	Mass (Da)
	Q8G2Q0 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 6CFFAAE9739604FB	FASTA	100	11,129
	10 20 30 40 50 60 MNLTPREKDK LLIAMAAMVA RRRLERGVKL NHPEAIALVS DFVVEGARDG RTVAELMEAG 70 80 90 100 AYVITREQVM DGVAEMIRDI QVEATFPDGT KLVTVHEPIR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.
[2]	"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed: 22038969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.
[3]	"Brucella suis urease encoded by ure1 but not ure2 is necessary for intestinal infection of BALB/c mice." Bandara A.B., Contreras A., Contreras-Rodriguez A., Martins A.M., Dobrean V., Poff-Reichow S., Rajasekaran P., Sriranganathan N., Schurig G.G., Boyle S.M. BMC Microbiol. 7:57-57(2007) [PubMed: 17578575] [Abstract] Cited for: CHARACTERIZATION OF ROLE IN VIRULENCE, OPERON DISRUPTION. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN29217.1. CP002997 Genomic DNA. Translation: AEM17630.1.
RefSeq	NP_697302.1. NC_004310.3.
3D structure databases
ProteinModelPortal	Q8G2Q0.
SMR	Q8G2Q0. Positions 1-99.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1165928.
GenomeReviews	Gene locus BR0268 in contig AE014291_GR.
KEGG	bms:BR0268.
NMPDR	fig|204722.1.peg.259.
PATRIC	17788823. VBIBruSui107850_0281.
TIGR	BR0268.
Phylogenomic databases
HOGENOM	HBG286300.
OMA	HVITRDQ.
ProtClustDB	PRK13241.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0268-MONOMER.
Family and domain databases
HAMAP	MF_00739. Urease_gamma. [Tree]
InterPro	IPR002026. Urease_gamma/gamma-beta_su. IPR012010. Urease_gamma_su. [Graphical view]
Gene3D	G3DSA:3.30.280.10. Urease_gamma_reg. 1 hit.
KO	K01430.
Pfam	PF00547. Urease_gamma. 1 hit. [Graphical view]
PIRSF	PIRSF001223. Urease_gamma. 1 hit.
ProDom	PD002319. Urease_gamma_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF54111. Urease_gamma_reg. 1 hit.
TIGRFAMs	TIGR00193. Urease_gam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

URE31_BRUSU

Accession

Primary (citable) accession number: Q8G2Q0
Secondary accession number(s): G0KBW7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program