Q8G2Q0 (URE31_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Urease subunit gamma 1
EC=3.5.1.5

Alternative name(s):
Urea amidohydrolase subunit gamma 1

Gene names

Name:	ureA1
Synonyms:	ureA-1
Ordered Locus Names:	BR0268, BS1330_I0269

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella suis

Protein attributes

Sequence length	100 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Disrupting the ure1 operon causes loss of urease activity, decreased resistance to low pH killing in vitro and decreased pathogen survival when innoculated in BALB/c mice by gavage. Ref.3
Catalytic activity	Urea + H2O = CO2 + 2 NH3. HAMAP-Rule MF_00739
Pathway	Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1. HAMAP-Rule MF_00739
Subunit structure	Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the urease gamma subunit family.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW urea catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	nickel cation binding Inferred from electronic annotation. Source: InterPro urease activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 100	100	Urease subunit gamma 1 HAMAP-Rule MF_00739		PRO_0000098003

Sequences

	Sequence		Length	Mass (Da)
	Q8G2Q0 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 6CFFAAE9739604FB	FASTA	100	11,129
	10 20 30 40 50 60 MNLTPREKDK LLIAMAAMVA RRRLERGVKL NHPEAIALVS DFVVEGARDG RTVAELMEAG 70 80 90 100 AYVITREQVM DGVAEMIRDI QVEATFPDGT KLVTVHEPIR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.
[2]	"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.
[3]	"Brucella suis urease encoded by ure1 but not ure2 is necessary for intestinal infection of BALB/c mice." Bandara A.B., Contreras A., Contreras-Rodriguez A., Martins A.M., Dobrean V., Poff-Reichow S., Rajasekaran P., Sriranganathan N., Schurig G.G., Boyle S.M. BMC Microbiol. 7:57-57(2007) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION OF ROLE IN VIRULENCE, OPERON DISRUPTION. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN29217.1. CP002997 Genomic DNA. Translation: AEM17630.1.
RefSeq	NP_697302.1. NC_004310.3. YP_005615122.1. NC_017251.1.
3D structure databases
ProteinModelPortal	Q8G2Q0.
SMR	Q8G2Q0. Positions 1-99.
ModBase	Search...
Protein-protein interaction databases
STRING	204722.BR0268.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN29217; AAN29217; BR0268. AEM17630; AEM17630; BS1330_I0269.
GeneID	1165928. 12136587.
KEGG	bms:BR0268. bsi:BS1330_I0269.
PATRIC	17788823. VBIBruSui107850_0281.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0831.
HOGENOM	HOG000075053.
KO	K01430.
OMA	EAIAIIS.
ProtClustDB	PRK13241.
Enzyme and pathway databases
UniPathway	UPA00258; UER00370.
Family and domain databases
Gene3D	3.30.280.10. 1 hit.
HAMAP	MF_00739. Urease_gamma.
InterPro	IPR002026. Urease_gamma/gamma-beta_su. IPR012010. Urease_gamma_su. [Graphical view]
Pfam	PF00547. Urease_gamma. 1 hit. [Graphical view]
PIRSF	PIRSF001223. Urease_gamma. 1 hit.
ProDom	PD002319. Urease_gamma_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF54111. Urease_gamma_reg. 1 hit.
TIGRFAMs	TIGR00193. urease_gam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

URE31_BRUSU

Accession

Primary (citable) accession number: Q8G2Q0
Secondary accession number(s): G0KBW7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program