Reviewed, UniProtKB/Swiss-Prot Q8G2P9 (URE21_BRUSU)

Names and origin

Protein names

Recommended name:
    Urease subunit beta 1
    EC=3.5.1.5
Alternative name(s):
    Urea amidohydrolase subunit beta 1

Gene names

Name:	ureB1
Ordered Locus Names:	BR0269

Organism

Brucella suis [Complete proteome] [HAMAP]

Taxonomic identifier

29461 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	101 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Disrupting the ure1 operon causes loss of urease activity, decreased resistance to low pH killing in vitro and decreased pathogen survival when innoculated in BALB/c mice by gavage. Ref.2
Catalytic activity	Urea + H2O = CO2 + 2 NH3. HAMAP MF_01954
Pathway	Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1. HAMAP MF_01954
Subunit structure	Heterotrimer of ureA (gamma), ureB (beta) and ureC (alpha) subunits. Three heterotrimers associate to form the active enzyme By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the urease beta subunit family.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nitrogen compound metabolic process Inferred from electronic annotation. Source: InterPro pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel ion binding Inferred from electronic annotation. Source: InterPro urease activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 101	101	Urease subunit beta 1 HAMAP MF_01954		PRO_0000234238

Sequences

	Sequence		Length	Mass (Da)
	Q8G2P9-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: EC6F5DA786479D39	FASTA	101	11,337
	10 20 30 40 50 60 MIPGEIITLE GDIELNQGQP TVTMRVANTG DRPIQVGSHF HFYEVNAALS FDREKARGQR 70 80 90 100 LDIAAGTAVR FEPGQERDVT LVPIRGHREI YGFRQMIMGK L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330 / Biovar 1.
[2]	"Brucella suis urease encoded by ure1 but not ure2 is necessary for intestinal infection of BALB/c mice." Bandara A.B., Contreras A., Contreras-Rodriguez A., Martins A.M., Dobrean V., Poff-Reichow S., Rajasekaran P., Sriranganathan N., Schurig G.G., Boyle S.M. BMC Microbiol. 7:57-57(2007) [PubMed: 17578575] [Abstract] Cited for: CHARACTERIZATION OF ROLE IN VIRULENCE, OPERON DISRUPTION. Strain: 1330 / Biovar 1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014291 Genomic DNA. Translation: AAN29218.1.
RefSeq	NP_697303.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1165929.
GenomeReviews	Gene locus BR0269 in contig AE014291_GR.
KEGG	bms:BR0269.
TIGR	BR0269.
Phylogenomic databases
HOGENOM	Q8G2P9.
OMA	PGELELN
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0269-MON.
BRENDA	3.5.1.5. 281610.
Family and domain databases
HAMAP	MF_01954. [Tree]
InterPro	IPR002019. Urease_beta. [Graphical view]
Gene3D	G3DSA:2.10.150.10. Urease_beta. 1 hit.
Pfam	PF00699. Urease_beta. 1 hit. [Graphical view]
TIGRFAMs	TIGR00192. urease_beta. 1 hit.
ProtoNet	Search...

Entry information

Entry name

URE21_BRUSU

Accession

Primary (citable) accession number: Q8G2P9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	March 1, 2003
Last modified:	November 24, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)