Q8G2P9 (URE21_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Urease subunit beta 1
EC=3.5.1.5

Alternative name(s):
Urea amidohydrolase subunit beta 1

Gene names

Name:	ureB1
Ordered Locus Names:	BR0269, BS1330_I0270

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella suis

Protein attributes

Sequence length	101 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Disrupting the ure1 operon causes loss of urease activity, decreased resistance to low pH killing in vitro and decreased pathogen survival when innoculated in BALB/c mice by gavage. Ref.3
Catalytic activity	Urea + H2O = CO2 + 2 NH3. HAMAP-Rule MF_01954
Pathway	Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1. HAMAP-Rule MF_01954
Subunit structure	Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the urease beta subunit family.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW urea catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	urease activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 101	101	Urease subunit beta 1 HAMAP-Rule MF_01954		PRO_0000234238

Sequences

	Sequence		Length	Mass (Da)
	Q8G2P9 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: EC6F5DA786479D39	FASTA	101	11,337
	10 20 30 40 50 60 MIPGEIITLE GDIELNQGQP TVTMRVANTG DRPIQVGSHF HFYEVNAALS FDREKARGQR 70 80 90 100 LDIAAGTAVR FEPGQERDVT LVPIRGHREI YGFRQMIMGK L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.
[2]	"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.
[3]	"Brucella suis urease encoded by ure1 but not ure2 is necessary for intestinal infection of BALB/c mice." Bandara A.B., Contreras A., Contreras-Rodriguez A., Martins A.M., Dobrean V., Poff-Reichow S., Rajasekaran P., Sriranganathan N., Schurig G.G., Boyle S.M. BMC Microbiol. 7:57-57(2007) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION OF ROLE IN VIRULENCE, OPERON DISRUPTION. Strain: 1330.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN29218.1. CP002997 Genomic DNA. Translation: AEM17631.1.
RefSeq	NP_697303.1. NC_004310.3. YP_005615123.1. NC_017251.1.
3D structure databases
ProteinModelPortal	Q8G2P9.
SMR	Q8G2P9. Positions 1-101.
ModBase	Search...
Protein-protein interaction databases
STRING	204722.BR0269.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN29218; AAN29218; BR0269. AEM17631; AEM17631; BS1330_I0270.
GeneID	1165929. 12136588.
KEGG	bms:BR0269. bsi:BS1330_I0270.
PATRIC	17788827. VBIBruSui107850_0283.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0832.
HOGENOM	HOG000077770.
KO	K01429.
OMA	REVYGFQ.
ProtClustDB	PRK13203.
Enzyme and pathway databases
UniPathway	UPA00258; UER00370.
Family and domain databases
Gene3D	2.10.150.10. 1 hit.
HAMAP	MF_01954. Urease_beta.
InterPro	IPR002019. Urease_beta. [Graphical view]
Pfam	PF00699. Urease_beta. 1 hit. [Graphical view]
SUPFAM	SSF51278. Urease_beta. 1 hit.
TIGRFAMs	TIGR00192. urease_beta. 1 hit.
ProtoNet	Search...

Entry information

Entry name

URE21_BRUSU

Accession

Primary (citable) accession number: Q8G2P9
Secondary accession number(s): G0KBW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program