Q8G2P7 (UREE1_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Urease accessory protein UreE 1

Gene names

Name:	ureE1
Synonyms:	ureE
Ordered Locus Names:	BR0271, BS1330_I0272

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	162 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in urease metallocenter assembly. Binds nickel. Probably functions as a nickel donor during metallocenter assembly By similarity. Ref.3
Subcellular location	Cytoplasm By similarity HAMAP MF_00822.
Disruption phenotype	Disrupting the ure1 operon causes loss of urease activity, decreased resistance to low pH killing in vitro and decreased pathogen survival when innoculated in BALB/c mice by gavage. Ref.3
Sequence similarities	Belongs to the UreE family.

Ontologies

Keywords
Biological process	Nickel insertion Virulence
Cellular component	Cytoplasm
Ligand	Nickel
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	enzyme active site formation Inferred from electronic annotation. Source: InterPro pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW protein complex assembly Inferred from electronic annotation. Source: InterPro protein folding Inferred from electronic annotation. Source: InterPro urea metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 162	162	Urease accessory protein UreE 1 HAMAP MF_00822		PRO_0000223407

Sequences

	Sequence		Length	Mass (Da)
	Q8G2P7 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: C65F2F78A6F486FD	FASTA	162	18,481
	10 20 30 40 50 60 MFRAIAIIRA HEVIDAVPAS HIVLERDERH LRRKAITLEN GEKILADFAE PVVLEHGDRL 70 80 90 100 110 120 VLDDGREIEI RAASEELYEI RGRDPRHIAE LAWHIGNRHL AAQIETDRIF ILRDHVIRVM 130 140 150 160 LEGLGATVTD VVAIFSPLRG AYSGGHQHHH GHDHDHHHPD HE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.
[2]	"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed: 22038969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.
[3]	"Brucella suis urease encoded by ure1 but not ure2 is necessary for intestinal infection of BALB/c mice." Bandara A.B., Contreras A., Contreras-Rodriguez A., Martins A.M., Dobrean V., Poff-Reichow S., Rajasekaran P., Sriranganathan N., Schurig G.G., Boyle S.M. BMC Microbiol. 7:57-57(2007) [PubMed: 17578575] [Abstract] Cited for: CHARACTERIZATION OF ROLE IN VIRULENCE, DISRUPTION PHENOTYPE. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN29220.1. CP002997 Genomic DNA. Translation: AEM17633.1.
RefSeq	NP_697305.1. NC_004310.3.
3D structure databases
ProteinModelPortal	Q8G2P7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1165931.
GenomeReviews	Gene locus BR0271 in contig AE014291_GR.
KEGG	bms:BR0271.
NMPDR	fig|204722.1.peg.262.
PATRIC	17788831. VBIBruSui107850_0285.
TIGR	BR0271.
Phylogenomic databases
HOGENOM	HBG635302.
OMA	DARCKSR.
ProtClustDB	PRK13261.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0271-MONOMER.
Family and domain databases
HAMAP	MF_00822. UreE. [Tree]
InterPro	IPR012406. UreE. IPR007864. UreE_C. IPR004029. UreE_N. [Graphical view]
Gene3D	G3DSA:3.30.70.790. UreE_C. 1 hit.
KO	K03187.
Pfam	PF05194. UreE_C. 1 hit. PF02814. UreE_N. 1 hit. [Graphical view]
PIRSF	PIRSF036402. Ureas_acces_UreE. 1 hit.
SMART	SM00988. UreE_N. 1 hit. [Graphical view]
SUPFAM	SSF69737. UreE_C. 1 hit. SSF69287. UreE_N. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UREE1_BRUSU

Accession

Primary (citable) accession number: Q8G2P7
Secondary accession number(s): G0KBX0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2006
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program