Q8G2P5 (UREG1_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Urease accessory protein UreG 1

Gene names

Name:	ureG1
Synonyms:	ureG-1
Ordered Locus Names:	BR0273, BS1330_I0274

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Facilitates the functional incorporation of the urease nickel metallocenter. This process requires GTP hydrolysis, probably effectuated by UreG By similarity. Ref.3 Disrupting the ure1 operon causes loss of urease activity, decreased resistance to low pH killing in vitro and decreased pathogen survival when innoculated in BALB/c mice by gavage. Ref.3
Subunit structure	Homodimer. UreD, UreF and UreG form a complex that acts as a GTP-hydrolysis-dependent molecular chaperone, activating the urease apoprotein by helping to assemble the nickel containing metallocenter of UreC. The UreE protein probably delivers the nickel By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_01389.
Sequence similarities	Belongs to the UreG family.

Ontologies

Keywords
Biological process	Nickel insertion Virulence
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro GTP binding Inferred from electronic annotation. Source: UniProtKB-KW nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 208	208	Urease accessory protein UreG 1 HAMAP MF_01389		PRO_0000347363
Regions
	Nucleotide binding	14 – 21	8	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8G2P5 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 39FF82812CA4FDE2	FASTA	208	22,705
	10 20 30 40 50 60 MTQKNGPLRV GIGGPVGSGK TTLTEKLCKA MRDKYSVAVI TNDIYTQEDA LILARRQALS 70 80 90 100 110 120 EDRIIGVETG GCPHTAIRED ASINLQAVVE MTRRFPDLDV VFIESGGDNL AATFSPDLAD 130 140 150 160 170 180 LTLYVISVCQ GEEIPRKGGP GITRSDFLVI NKSDLAPYVH VDLEVMEADA MRMRAKRPFG 190 200 FTDLHRGKGV QEIIDFIVEN GGLEPRSN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.
[2]	"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed: 22038969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.
[3]	"Brucella suis urease encoded by ure1 but not ure2 is necessary for intestinal infection of BALB/c mice." Bandara A.B., Contreras A., Contreras-Rodriguez A., Martins A.M., Dobrean V., Poff-Reichow S., Rajasekaran P., Sriranganathan N., Schurig G.G., Boyle S.M. BMC Microbiol. 7:57-57(2007) [PubMed: 17578575] [Abstract] Cited for: OPERON DISRUPTION, ROLE IN VIRULENCE. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN29222.1. CP002997 Genomic DNA. Translation: AEM17635.1.
PIR	AC3458.
RefSeq	NP_697307.1. NC_004310.3.
3D structure databases
ProteinModelPortal	Q8G2P5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1165933.
GenomeReviews	Gene locus BR0273 in contig AE014291_GR.
KEGG	bms:BR0273.
PATRIC	17788835. VBIBruSui107850_0287.
TIGR	BR0273.
Phylogenomic databases
HOGENOM	HBG364734.
OMA	PFIFTNL.
PhylomeDB	Q8G2P5.
ProtClustDB	CLSK864148.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0273-MONOMER.
Family and domain databases
HAMAP	MF_01389. UreG. [Tree]
InterPro	IPR003495. Cbl_biosynth_CobW-like. IPR012202. NiFe-hyd/urease_mat_GTPase. IPR004400. Urease_accessory_UreG. [Graphical view]
KO	K03189.
Pfam	PF02492. cobW. 1 hit. [Graphical view]
PIRSF	PIRSF005624. Ni-bind_GTPase. 1 hit.
TIGRFAMs	TIGR00101. UreG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UREG1_BRUSU

Accession

Primary (citable) accession number: Q8G2P5
Secondary accession number(s): G0KBX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program