Reviewed, UniProtKB/Swiss-Prot Q8G2L7 (OTC_BRUSU)

Names and origin

Protein names

Recommended name:
    Ornithine carbamoyltransferase
      Short name=OTCase
    EC=2.1.3.3

Gene names

Name:	argF
Ordered Locus Names:	BR0302

Organism

Brucella suis [Complete proteome] [HAMAP]

Taxonomic identifier

29461 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline. HAMAP MF_01109
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3. HAMAP MF_01109
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ornithine carbamoyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro ornithine carbamoyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Ornithine carbamoyltransferase HAMAP MF_01109		PRO_0000112896
Regions
	Region	57 – 61	5	Carbamoyl phosphate binding By similarity
	Region	264 – 267	4	Ornithine binding By similarity
Sites
	Binding site	108	1	Carbamoyl phosphate By similarity
	Binding site	135	1	Carbamoyl phosphate By similarity
	Site	32	1	Important for structural integrity By similarity
	Site	148	1	Important for structural integrity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8G2L7-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 9487532632E05CB4	FASTA	312	34,193
	10 20 30 40 50 60 MANDNGIKHF IDLSTVPATE LRAILEDAKA RKARLKAGEV ERPYAGKVLA MIFEKPSTRT 70 80 90 100 110 120 RVSFDVGMRQ LGGETIMLTG SEMQLGRSEA IADTAKVLSR YVDAIMIRTT AHERMLELAE 130 140 150 160 170 180 YATVPVINAL TDDTHPCQIM ADVLTYEEHR GPIKGKTFAW MGDGNNVLHS LVEAAARFDF 190 200 210 220 230 240 NVNIATPKGS EPKSQYIDWA RANGAGIMST TDPEKAASGA DCIVTDTWVS MGQEDHARGH 250 260 270 280 290 300 NVFIPYQVNA NLMAKADPKA LFMHCLPAHR GEEVTDEVID GPQSVVFDEA ENRLHAQKAI 310 LAWCLQDRGL GA

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014291 Genomic DNA. Translation: AAN29251.1.
RefSeq	NP_697336.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1165963.
GenomeReviews	Gene locus BR0302 in contig AE014291_GR.
KEGG	bms:BR0302.
NMPDR	fig|204722.1.peg.293.
TIGR	BR0302.
Phylogenomic databases
HOGENOM	Q8G2L7.
OMA	CVVTDTW
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0302-MON.
BRENDA	2.1.3.3. 281610.
Family and domain databases
HAMAP	MF_01109. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P_bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn_bd. IPR002292. Orn_carbamltrans. [Graphical view]
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00102. OTCASE.
TIGRFAMs	TIGR00658. orni_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTC_BRUSU

Accession

Primary (citable) accession number: Q8G2L7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2003
Last sequence update:	March 1, 2003
Last modified:	November 24, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)