Q8G2K1 (MSCL_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Large-conductance mechanosensitive channel

Gene names

Name:	mscL
Ordered Locus Names:	BR0318, BS1330_I0319

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella suis

Protein attributes

Sequence length	138 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell By similarity. HAMAP-Rule MF_00115
Subunit structure	Homopentamer By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_00115.
Sequence similarities	Belongs to the MscL family.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Ion channel
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ion channel activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 138	138	Large-conductance mechanosensitive channel HAMAP-Rule MF_00115		PRO_0000192436
Regions
	Transmembrane	15 – 35	21	Helical; Potential
	Transmembrane	38 – 58	21	Helical; Potential
	Transmembrane	80 – 100	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8G2K1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 64ADC7B8F6239A96	FASTA	138	14,899
	10 20 30 40 50 60 MLKEFQEFAL KGNMVDLAIG VIIGGAFGGL VNSIVNDIIM PIIGLITGGI DFSNMFIQLA 70 80 90 100 110 120 GDPKTTLAAA REAGATIAYG NFITLLINFL IIAWVLFLVV KLMNRLKKRE EAKPAPAAPS 130 EEVLLTEIRD ILAKQQKA

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN29267.1. CP002997 Genomic DNA. Translation: AEM17680.1.
RefSeq	NP_697352.1. NC_004310.3. YP_005615172.1. NC_017251.1.
3D structure databases
ProteinModelPortal	Q8G2K1.
ModBase	Search...
Protein-protein interaction databases
STRING	204722.BR0318.
Proteomic databases
PRIDE	Q8G2K1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN29267; AAN29267; BR0318. AEM17680; AEM17680; BS1330_I0319.
GeneID	1165979. 12136638.
KEGG	bms:BR0318. bsi:BS1330_I0319.
PATRIC	17788925. VBIBruSui107850_0331.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1970.
HOGENOM	HOG000226782.
KO	K03282.
OMA	KEEPKIS.
ProtClustDB	PRK13952.
Family and domain databases
HAMAP	MF_00115. MscL.
InterPro	IPR019823. Mechanosensitive_channel_CS. IPR001185. MS_channel. [Graphical view]
Pfam	PF01741. MscL. 1 hit. [Graphical view]
PRINTS	PR01264. MECHCHANNEL.
SUPFAM	SSF81330. MS_channel. 1 hit.
TIGRFAMs	TIGR00220. mscL. 1 hit.
PROSITE	PS01327. MSCL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MSCL_BRUSU

Accession

Primary (citable) accession number: Q8G2K1
Secondary accession number(s): G0K674

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program