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Protein

Trans-3-hydroxy-L-proline dehydratase

Gene

BR0337

Organism
Brucella suis biovar 1 (strain 1330)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the dehydration of trans-3-hydroxy-L-proline (t3LHyp) to Delta(1)-pyrroline-2-carboxylate (Pyr2C) (PubMed:24980702). Displays neither proline racemase activity nor 4-hydroxyproline 2-epimerase activity (PubMed:17849014, PubMed:24980702).2 Publications

Catalytic activityi

Trans-3-hydroxy-L-proline = 1-pyrroline 2-carboxylate + H2O.1 Publication

Kineticsi

kcat is 17 sec(-1) for t3LHyp dehydration.1 Publication

  1. KM=5.1 mM for trans-3-hydroxy-L-proline1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei90 – 901Proton acceptorBy similarity
    Binding sitei251 – 2511SubstrateBy similarity

    GO - Molecular functioni

    • proline racemase activity Source: InterPro
    • trans-L-3-hydroxyproline dehydratase activity Source: CACAO

    GO - Biological processi

    • metabolic process Source: GOC
    Complete GO annotation...

    Keywords - Molecular functioni

    Lyase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trans-3-hydroxy-L-proline dehydratase1 Publication (EC:4.2.1.771 Publication)
    Short name:
    T3LHyp dehydratase
    Short name:
    t3HypD1 Publication
    Alternative name(s):
    Trans-L-3-hydroxyproline dehydratase
    Gene namesi
    Ordered Locus Names:BR0337, BS1330_I0338
    OrganismiBrucella suis biovar 1 (strain 1330)
    Taxonomic identifieri204722 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000007104 Componenti: Chromosome I

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 342342Trans-3-hydroxy-L-proline dehydratasePRO_0000354045Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ8G2I3.
    SMRiQ8G2I3. Positions 1-332.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni91 – 922Substrate bindingBy similarity
    Regioni256 – 2572Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the proline racemase family.Curated

    Phylogenomic databases

    eggNOGiCOG3938.
    HOGENOMiHOG000084336.
    OMAiWITGIHQ.
    OrthoDBiEOG6SNDV3.

    Family and domain databases

    InterProiIPR008794. Pro_racemase_fam.
    [Graphical view]
    PfamiPF05544. Pro_racemase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF029792. Pro_racemase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8G2I3-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRSTKVIHIV GCHAEGEVGD VIVGGVAPPP GETVWEQSRF IANDETLRNF
    60 70 80 90 100
    VLNEPRGGVF RHVNLLVPPK DPRAQMGFII MEPADTPPMS GSNSICVSTV
    110 120 130 140 150
    LLDSGIIAMQ EPVTHMVLEA PGGIIEVEAE CRNGKAERIS VRNVPSFADR
    160 170 180 190 200
    LDAPLDVTGL GTIMVDTAYG GDSFVIVDAA QIGMKIEPGQ ARELAEIGVK
    210 220 230 240 250
    ITKAANEQLG FRHPERDWRH ISFCQITEPV TREGDVLTGV NTVAIRPAKL
    260 270 280 290 300
    DRSPTGTGCS ARMAVLHAKG QMKAGERFIG KSVLGTEFHC RLDKVLELGG
    310 320 330 340
    KPAISPIISG RAWVTGTSQL MLDPSDPFPH GYRLSDTWPR DE
    Length:342
    Mass (Da):36,941
    Last modified:March 1, 2003 - v1
    Checksum:i1982D458A78AAE8A
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE014291 Genomic DNA. Translation: AAN29286.1.
    CP002997 Genomic DNA. Translation: AEM17699.1.
    RefSeqiWP_002966688.1. NZ_KN046804.1.

    Genome annotation databases

    EnsemblBacteriaiAAN29286; AAN29286; BR0337.
    AEM17699; AEM17699; BS1330_I0338.
    GeneIDi3787134.
    KEGGibms:BR0337.
    bsi:BS1330_I0338.
    PATRICi17788965. VBIBruSui107850_0351.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE014291 Genomic DNA. Translation: AAN29286.1.
    CP002997 Genomic DNA. Translation: AEM17699.1.
    RefSeqiWP_002966688.1. NZ_KN046804.1.

    3D structure databases

    ProteinModelPortaliQ8G2I3.
    SMRiQ8G2I3. Positions 1-332.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAN29286; AAN29286; BR0337.
    AEM17699; AEM17699; BS1330_I0338.
    GeneIDi3787134.
    KEGGibms:BR0337.
    bsi:BS1330_I0338.
    PATRICi17788965. VBIBruSui107850_0351.

    Phylogenomic databases

    eggNOGiCOG3938.
    HOGENOMiHOG000084336.
    OMAiWITGIHQ.
    OrthoDBiEOG6SNDV3.

    Family and domain databases

    InterProiIPR008794. Pro_racemase_fam.
    [Graphical view]
    PfamiPF05544. Pro_racemase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF029792. Pro_racemase. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1330.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1330.
    3. "Molecular and structural discrimination of proline racemase and hydroxyproline-2-epimerase from nosocomial and bacterial pathogens."
      Goytia M., Chamond N., Cosson A., Coatnoan N., Hermant D., Berneman A., Minoprio P.
      PLoS ONE 2:E885-E885(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: LACK OF ENZYMATIC ACTIVITY AS PROLINE RACEMASE AND HYDROXYPROLINE 2-EPIMERASE.
      Strain: 1330.
    4. "Prediction and characterization of enzymatic activities guided by sequence similarity and genome neighborhood networks."
      Zhao S., Sakai A., Zhang X., Vetting M.W., Kumar R., Hillerich B., San Francisco B., Solbiati J., Steves A., Brown S., Akiva E., Barber A., Seidel R.D., Babbitt P.C., Almo S.C., Gerlt J.A., Jacobson M.P.
      Elife 3:0-0(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiT3HPD_BRUSU
    AccessioniPrimary (citable) accession number: Q8G2I3
    Secondary accession number(s): G0K693
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 25, 2008
    Last sequence update: March 1, 2003
    Last modified: July 22, 2015
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Brucella suis
      Brucella suis (strain 1330): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.