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Protein

Trans-3-hydroxy-L-proline dehydratase

Gene

BR0337

Organism
Brucella suis biovar 1 (strain 1330)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the dehydration of trans-3-hydroxy-L-proline (t3LHyp) to Delta(1)-pyrroline-2-carboxylate (Pyr2C) (PubMed:24980702). Displays neither proline racemase activity nor 4-hydroxyproline 2-epimerase activity (PubMed:17849014, PubMed:24980702).2 Publications

Catalytic activityi

Trans-3-hydroxy-L-proline = 1-pyrroline 2-carboxylate + H2O.1 Publication

Kineticsi

kcat is 17 sec(-1) for t3LHyp dehydration.1 Publication

  1. KM=5.1 mM for trans-3-hydroxy-L-proline1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei90 – 901Proton acceptorBy similarity
Binding sitei251 – 2511SubstrateBy similarity

GO - Molecular functioni

  1. proline racemase activity Source: InterPro
  2. trans-L-3-hydroxyproline dehydratase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Names & Taxonomyi

Protein namesi
Recommended name:
Trans-3-hydroxy-L-proline dehydratase1 Publication (EC:4.2.1.771 Publication)
Short name:
T3LHyp dehydratase
Short name:
t3HypD1 Publication
Alternative name(s):
Trans-L-3-hydroxyproline dehydratase
Gene namesi
Ordered Locus Names:BR0337, BS1330_I0338
OrganismiBrucella suis biovar 1 (strain 1330)
Taxonomic identifieri204722 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000007104 Componenti: Chromosome I UP000000824 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 342342Trans-3-hydroxy-L-proline dehydratasePRO_0000354045Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi204722.BR0337.

Structurei

3D structure databases

ProteinModelPortaliQ8G2I3.
SMRiQ8G2I3. Positions 1-332.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni91 – 922Substrate bindingBy similarity
Regioni256 – 2572Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the proline racemase family.Curated

Phylogenomic databases

eggNOGiCOG3938.
HOGENOMiHOG000084336.
OMAiPMKEPIT.
OrthoDBiEOG6SNDV3.

Family and domain databases

InterProiIPR008794. Pro_racemase_fam.
[Graphical view]
PfamiPF05544. Pro_racemase. 1 hit.
[Graphical view]
PIRSFiPIRSF029792. Pro_racemase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8G2I3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSTKVIHIV GCHAEGEVGD VIVGGVAPPP GETVWEQSRF IANDETLRNF
60 70 80 90 100
VLNEPRGGVF RHVNLLVPPK DPRAQMGFII MEPADTPPMS GSNSICVSTV
110 120 130 140 150
LLDSGIIAMQ EPVTHMVLEA PGGIIEVEAE CRNGKAERIS VRNVPSFADR
160 170 180 190 200
LDAPLDVTGL GTIMVDTAYG GDSFVIVDAA QIGMKIEPGQ ARELAEIGVK
210 220 230 240 250
ITKAANEQLG FRHPERDWRH ISFCQITEPV TREGDVLTGV NTVAIRPAKL
260 270 280 290 300
DRSPTGTGCS ARMAVLHAKG QMKAGERFIG KSVLGTEFHC RLDKVLELGG
310 320 330 340
KPAISPIISG RAWVTGTSQL MLDPSDPFPH GYRLSDTWPR DE
Length:342
Mass (Da):36,941
Last modified:February 28, 2003 - v1
Checksum:i1982D458A78AAE8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN29286.1.
CP002997 Genomic DNA. Translation: AEM17699.1.
RefSeqiNP_697371.1. NC_004310.3.
YP_005615191.1. NC_017251.1.

Genome annotation databases

EnsemblBacteriaiAAN29286; AAN29286; BR0337.
AEM17699; AEM17699; BS1330_I0338.
KEGGibms:BR0337.
bsi:BS1330_I0338.
PATRICi17788965. VBIBruSui107850_0351.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN29286.1.
CP002997 Genomic DNA. Translation: AEM17699.1.
RefSeqiNP_697371.1. NC_004310.3.
YP_005615191.1. NC_017251.1.

3D structure databases

ProteinModelPortaliQ8G2I3.
SMRiQ8G2I3. Positions 1-332.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi204722.BR0337.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN29286; AAN29286; BR0337.
AEM17699; AEM17699; BS1330_I0338.
KEGGibms:BR0337.
bsi:BS1330_I0338.
PATRICi17788965. VBIBruSui107850_0351.

Phylogenomic databases

eggNOGiCOG3938.
HOGENOMiHOG000084336.
OMAiPMKEPIT.
OrthoDBiEOG6SNDV3.

Family and domain databases

InterProiIPR008794. Pro_racemase_fam.
[Graphical view]
PfamiPF05544. Pro_racemase. 1 hit.
[Graphical view]
PIRSFiPIRSF029792. Pro_racemase. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1330.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1330.
  3. "Molecular and structural discrimination of proline racemase and hydroxyproline-2-epimerase from nosocomial and bacterial pathogens."
    Goytia M., Chamond N., Cosson A., Coatnoan N., Hermant D., Berneman A., Minoprio P.
    PLoS ONE 2:E885-E885(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: LACK OF ENZYMATIC ACTIVITY AS PROLINE RACEMASE AND HYDROXYPROLINE 2-EPIMERASE.
    Strain: 1330.
  4. "Prediction and characterization of enzymatic activities guided by sequence similarity and genome neighborhood networks."
    Zhao S., Sakai A., Zhang X., Vetting M.W., Kumar R., Hillerich B., San Francisco B., Solbiati J., Steves A., Brown S., Akiva E., Barber A., Seidel R.D., Babbitt P.C., Almo S.C., Gerlt J.A., Jacobson M.P.
    Elife 3:0-0(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiT3HPD_BRUSU
AccessioniPrimary (citable) accession number: Q8G2I3
Secondary accession number(s): G0K693
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2008
Last sequence update: February 28, 2003
Last modified: March 31, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella suis
    Brucella suis (strain 1330): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.