Q8G2E8 (DER_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTPase Der

Alternative name(s):
GTP-binding protein EngA

Gene names

Name:	der
Synonyms:	engA
Ordered Locus Names:	BR0375, BS1330_I0376

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	483 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	GTPase that plays an essential role in the late steps of ribosome biogenesis By similarity. HAMAP MF_00195
Subunit structure	Associates with the 50S ribosomal subunit By similarity.
Sequence similarities	Belongs to the Era/MnmE GTP-binding protein family. Der subfamily. Contains 2 G (guanine nucleotide-binding) domains. Contains 1 KH-like domain.

Ontologies

Keywords
Biological process	Ribosome biogenesis
Domain	Repeat
Ligand	GTP-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ribosome biogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 483	483	GTPase Der HAMAP MF_00195		PRO_0000178973
Regions
	Domain	14 – 120	107	G 1
	Domain	223 – 331	109	G 2
	Domain	387 – 472	86	KH-like
	Nucleotide binding	9 – 16	8	GTP 1 Potential
	Nucleotide binding	56 – 60	5	GTP 1 Potential
	Nucleotide binding	119 – 122	4	GTP 1 Potential
	Nucleotide binding	218 – 225	8	GTP 2 Potential
	Nucleotide binding	265 – 269	5	GTP 2 Potential
	Nucleotide binding	330 – 333	4	GTP 2 Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8G2E8 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 776B950CFA036EF3	FASTA	483	53,281
	10 20 30 40 50 60 MGFTLAIVGR PNVGKSTLFN RLVGRKLALV DDLPGVTRDR RIHDAKLYDL KFQVIDTAGL 70 80 90 100 110 120 EEAANDSLEA RMRAQTEAAI SEADAVLFVI DAKAGITPAD STFAEAVRRS GKPVVLVANK 130 140 150 160 170 180 AEARGSEAGM YDAFQLGLGE PCPISAEHGQ GMPDLRDAIV ELLGEERVFA EERQEEAADE 190 200 210 220 230 240 VFTPAAVGAL VGDDIEDPDA EEIPAYDATK PLRIAIVGRP NAGKSTLINT MLGEDRLLTG 250 260 270 280 290 300 PEAGITRDSI SADWEWHGRK IKLFDTAGMR RKARVQEKLE KLSVADGLRA IRFAEVVIIV 310 320 330 340 350 360 LDATIPFEKQ DLQIADLIIR EGRAPVIAFN KWDLIEDRQM VLADLYEKTA RLLPQVRGLR 370 380 390 400 410 420 AVPISGERGQ GIDKLMENVV KTHEIWNRRI STGRLNRWLE GVIAHQPPPA VSGRRLKVKY 430 440 450 460 470 480 MTQVKTRPPG FVVSCSRPDA MPQSYVRYLI NGLRETFDMP GVPIRLSLRT SDNPFAGRAK KKK

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed: 22038969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN29321.1. CP002997 Genomic DNA. Translation: AEM17734.1.
RefSeq	NP_697406.1. NC_004310.3.
3D structure databases
ProteinModelPortal	Q8G2E8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1166036.
GenomeReviews	Gene locus BR0375 in contig AE014291_GR.
KEGG	bms:BR0375.
NMPDR	fig|204722.1.peg.363.
PATRIC	17789050. VBIBruSui107850_0393.
TIGR	BR0375.
Phylogenomic databases
HOGENOM	HBG592135.
OMA	LAVNKCE.
PhylomeDB	Q8G2E8.
ProtClustDB	PRK00093.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0375-MONOMER.
Family and domain databases
HAMAP	MF_00195. GTPase_Der. [Tree]
InterPro	IPR016484. GTP-bd_EngA. IPR006073. GTP_binding_domain. IPR015946. KH_dom-like_a/b. IPR005225. Small_GTP-bd_dom. [Graphical view]
Gene3D	G3DSA:3.30.300.20. KH_prok. 1 hit.
KO	K03977.
PANTHER	PTHR11649:SF5. PTHR11649:SF5. 1 hit.
Pfam	PF01926. MMR_HSR1. 2 hits. [Graphical view]
PIRSF	PIRSF006485. GTP-binding_EngA. 1 hit.
PRINTS	PR00326. GTP1OBG.
TIGRFAMs	TIGR03594. GTPase_EngA. 1 hit. TIGR00231. Small_GTP. 2 hits.
ProtoNet	Search...

Entry information

Entry name

DER_BRUSU

Accession

Primary (citable) accession number: Q8G2E8
Secondary accession number(s): G0K6J7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 24, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program