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Q8G2B1 (PUR2_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:BR0414, BS1330_I0415
OrganismBrucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]
Taxonomic identifier204722 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length427 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 427427Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151438

Regions

Domain107 – 312206ATP-grasp
Nucleotide binding133 – 19361ATP By similarity

Sites

Metal binding2821Magnesium or manganese By similarity
Metal binding2841Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8G2B1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 0CB320856F5D70CE

FASTA42744,971
        10         20         30         40         50         60 
MKVLLIGSGG REHALAWKLA ASPLLEKLYC APGNPGIAAV AELADIGVDD HAALIAFAKE 

        70         80         90        100        110        120 
KHIDLVVVGP EAPLVTGLAD EMRAEGIRVF GPSRAAAQLE GSKGFTKDLC ARFNIPTGAY 

       130        140        150        160        170        180 
GRFNNAPKAK AYIRQQGAPI VVKADGLAAG KGVVVAMTLQ EALDAVDSCF EGAFGAAGAE 

       190        200        210        220        230        240 
VVVEEFLDGE EASFFCICDG KTALPLGSAQ DHKRVGDGDT GPNTGGMGAY APAPVMTPEM 

       250        260        270        280        290        300 
VARTMRELIE PTMRGMAEIG APFSGILFAG LMITSDGPKL IEYNTRFGDP ECQVLMMRLN 

       310        320        330        340        350        360 
SDLLALINAA VDGRLDEVSL EWKDEPALTV VMAAEGYPAN VKKGSVIRDL EKLESIDGVK 

       370        380        390        400        410        420 
LFHAGTALKD GAIVAGGGRV LNITATAATV AQAQARAYEA LKLIDWPEGF YRSDIGWRAV 


EREKANR 

« Hide

References

[1]"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts."
Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. expand/collapse author list , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.
[2]"Revised genome sequence of Brucella suis 1330."
Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.
J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014291 Genomic DNA. Translation: AAN29359.1.
CP002997 Genomic DNA. Translation: AEM17772.1.
RefSeqNP_697444.1. NC_004310.3.
YP_005615264.1. NC_017251.1.

3D structure databases

ProteinModelPortalQ8G2B1.
SMRQ8G2B1. Positions 1-422.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING204722.BR0414.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN29359; AAN29359; BR0414.
AEM17772; AEM17772; BS1330_I0415.
GeneID1166075.
12136734.
KEGGbms:BR0414.
bsi:BS1330_I0415.
PATRIC17789124. VBIBruSui107850_0430.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033463.
KOK01945.
OMAYKGFLYA.
OrthoDBEOG69SKD1.

Enzyme and pathway databases

UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ8G2B1.

Entry information

Entry namePUR2_BRUSU
AccessionPrimary (citable) accession number: Q8G2B1
Secondary accession number(s): G0K6N5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Brucella suis

Brucella suis (strain 1330): entries and gene names