Q8G292 (DXS_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	BR0436, BS1330_I0437

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	643 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 643	643	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_0000189093

Sequences

	Sequence		Length	Mass (Da)
	Q8G292 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: E42DFB422D129FEA	FASTA	643	69,180
	10 20 30 40 50 60 MSRPSTPLLD KAPTPDRLRA LPEQDLPQLA EELRTELIDA VSTTGGHLGA GLGVVELTVA 70 80 90 100 110 120 LHHVFNTPYD RIIWDVGHQA YPHKILTGRR DRIRTLRQAG GLSGFTKRAE SEYDPFGAAH 130 140 150 160 170 180 SSTSISAGLG MAVASELSGE KRNVIAVIGD GSMSAGMAYE AMNNAGALDA RLIVILNDND 190 200 210 220 230 240 MSIAPPTGAM SAYLARLVSG RTYRSVREAA KQVAQKLPKF LQDKARKSEE YARAFFTGGT 250 260 270 280 290 300 LFEELGFYYV GPIDGHNLDH LLPVLKNVRD TQKGPVLIHV VTQKGKGYAP AEAAADKYHG 310 320 330 340 350 360 VNKFDVITGK QAKPPANVPS YTKIFGTSLI EEARHDDKIV AVTAAMPTGT GLDLFGEAFP 370 380 390 400 410 420 KRVFDVGIAE QHAVTFAAGL ASEGYKPFCA IYSTFLQRGY DQVVHDVSIQ NLPVRFPIDR 430 440 450 460 470 480 AGLVGADGPT HAGSFDTGFL AALPGFVVMA ASDEAELRHM VRTAAEYDEG PISFRYPRGD 490 500 510 520 530 540 GVGVDLPERG SVLEIGKGRI VREGTKVALL SFGTRLQECL AAAEELGAAG LSTTVADARF 550 560 570 580 590 600 AKPLDHDLIR RLAREHEVLV MVEEGAVGGF GSHVLQFLAT DGLLDRGLKV RALTLPDIYQ 610 620 630 640 DHGKPDAMYA EAGLDRTGIV RTVFAALHRD ELGHEALPTP FRA

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed: 22038969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN29379.1. CP002997 Genomic DNA. Translation: AEM17792.1.
RefSeq	NP_697464.1. NC_004310.3.
3D structure databases
ProteinModelPortal	Q8G292.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1166097.
GenomeReviews	Gene locus BR0436 in contig AE014291_GR.
KEGG	bms:BR0436.
NMPDR	fig|204722.1.peg.421.
PATRIC	17789166. VBIBruSui107850_0451.
TIGR	BR0436.
Phylogenomic databases
HOGENOM	HBG571647.
OMA	DPILYHG.
PhylomeDB	Q8G292.
ProtClustDB	PRK05444.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0436-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR001017. DH_E1. IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_BRUSU

Accession

Primary (citable) accession number: Q8G292
Secondary accession number(s): G0K6Q5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program