Reviewed, UniProtKB/Swiss-Prot Q8G290 (Y438_BRUSU)

Names and origin

Protein names

Recommended name:
    Uncharacterized RNA methyltransferase BR0438
    EC=2.1.1.-

Gene names

Ordered Locus Names:

BR0438

Organism

Brucella suis [Complete proteome] [HAMAP]

Taxonomic identifier

29461 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Sequence similarities

Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family.

Ontologies

Keywords
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW RNA methyltransferase activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	Uncharacterized RNA methyltransferase BR0438		PRO_0000161961
Sites
	Active site	370	1	Nucleophile By similarity
	Metal binding	66	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	72	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	75	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	149	1	Iron-sulfur (4Fe-4S) By similarity
	Binding site	249	1	S-adenosyl-L-methionine By similarity
	Binding site	276	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	296	1	S-adenosyl-L-methionine By similarity
	Binding site	344	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8G290-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: EFA906CE723331EB	FASTA	415	44,995
	10 20 30 40 50 60 MTSSKTGQIT IRSIGAGGDG VANLPDGQIY VPFTLPGEVV NVARDKNRAT LMALLEASPE 70 80 90 100 110 120 RQNPACRHFE DCGGCALQHW QDEPYRLWKR ELVVGALKGR GIDVEVAPLV ACNPHTRRRA 130 140 150 160 170 180 VFAARKTEKG VLLGFNRHQS HEIIDIVECP VTVPEIIARL DDLREVGALL APGSGPFKLA 190 200 210 220 230 240 ATLTESGLDL AASGCGKLND EQRRALTALV IKKDFARLSH EGEIIVEPKK PLIHFGKVPV 250 260 270 280 290 300 PVPPGCFLQA TAEAEETMAA LVLAHLGKAR RVADLFCGVG TFALRIAEKS AVHAVENDAA 310 320 330 340 350 360 ALAALDRGVR HVQGLKPVSI ERRDLFRRPL MPKELLPYNA VVFDPPRAGA EEQALELAKS 370 380 390 400 410 KVEKVVAISC NPVTLARDLA ILQKGGYRIE RVTPIDQFLW SAHVEAVAVL TKGRQ

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014291 Genomic DNA. Translation: AAN29381.1.
RefSeq	NP_697466.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1166099.
GenomeReviews	Gene locus BR0438 in contig AE014291_GR.
KEGG	bms:BR0438.
NMPDR	fig|204722.1.peg.423.
TIGR	BR0438.
Phylogenomic databases
HOGENOM	Q8G290.
OMA	CHELARS
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0438-MON.
Family and domain databases
InterPro	IPR016027. NA-bd_OB-fold-like. IPR010280. U5_MeTrfase. [Graphical view]
Pfam	PF05958. tRNA_U5-meth_tr. 1 hit. [Graphical view]
PROSITE	PS01230. TRMA_1. 1 hit. PS01231. TRMA_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Y438_BRUSU

Accession

Primary (citable) accession number: Q8G290

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	March 1, 2003
Last modified:	November 24, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)