Q8G290 (Y438_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized RNA methyltransferase BR0438/BS1330_I0439
EC=2.1.1.-

Gene names

Ordered Locus Names:

BR0438, BS1330_I0439

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Sequence similarities

Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family.

Ontologies

Keywords
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW RNA methyltransferase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	Uncharacterized RNA methyltransferase BR0438/BS1330_I0439		PRO_0000161961
Sites
	Active site	370	1	Nucleophile By similarity
	Metal binding	66	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	72	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	75	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	149	1	Iron-sulfur (4Fe-4S) By similarity
	Binding site	249	1	S-adenosyl-L-methionine By similarity
	Binding site	276	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	296	1	S-adenosyl-L-methionine By similarity
	Binding site	344	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8G290 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: EFA906CE723331EB	FASTA	415	44,995
	10 20 30 40 50 60 MTSSKTGQIT IRSIGAGGDG VANLPDGQIY VPFTLPGEVV NVARDKNRAT LMALLEASPE 70 80 90 100 110 120 RQNPACRHFE DCGGCALQHW QDEPYRLWKR ELVVGALKGR GIDVEVAPLV ACNPHTRRRA 130 140 150 160 170 180 VFAARKTEKG VLLGFNRHQS HEIIDIVECP VTVPEIIARL DDLREVGALL APGSGPFKLA 190 200 210 220 230 240 ATLTESGLDL AASGCGKLND EQRRALTALV IKKDFARLSH EGEIIVEPKK PLIHFGKVPV 250 260 270 280 290 300 PVPPGCFLQA TAEAEETMAA LVLAHLGKAR RVADLFCGVG TFALRIAEKS AVHAVENDAA 310 320 330 340 350 360 ALAALDRGVR HVQGLKPVSI ERRDLFRRPL MPKELLPYNA VVFDPPRAGA EEQALELAKS 370 380 390 400 410 KVEKVVAISC NPVTLARDLA ILQKGGYRIE RVTPIDQFLW SAHVEAVAVL TKGRQ

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed: 22038969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN29381.1. CP002997 Genomic DNA. Translation: AEM17794.1.
RefSeq	NP_697466.1. NC_004310.3.
3D structure databases
ProteinModelPortal	Q8G290.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1166099.
GenomeReviews	Gene locus BR0438 in contig AE014291_GR.
KEGG	bms:BR0438.
NMPDR	fig|204722.1.peg.423.
PATRIC	17789170. VBIBruSui107850_0453.
TIGR	BR0438.
Phylogenomic databases
HOGENOM	HBG690163.
OMA	ITIECLR.
PhylomeDB	Q8G290.
ProtClustDB	CLSK864749.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0438-MONOMER.
Family and domain databases
InterPro	IPR016027. NA-bd_OB-fold-like. IPR010280. U5_MeTrfase. [Graphical view]
KO	K03215.
Pfam	PF05958. tRNA_U5-meth_tr. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
PROSITE	PS01230. TRMA_1. 1 hit. PS01231. TRMA_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Y438_BRUSU

Accession

Primary (citable) accession number: Q8G290
Secondary accession number(s): G0K6Q7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program