Reviewed, UniProtKB/Swiss-Prot Q8G1Z8 (BETA_BRUSU)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	BR0553

Organism

Brucella suis [Complete proteome] [HAMAP]

Taxonomic identifier

29461 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity.
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP MF_00750
Cofactor	FAD By similarity.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP MF_00750
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 549	549	Choline dehydrogenase HAMAP MF_00750		PRO_0000205585
Regions
	Nucleotide binding	4 – 33	30	FAD Probable
Sites
	Active site	465	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8G1Z8-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: E045B27FEC88BA50	FASTA	549	60,623
	10 20 30 40 50 60 MEADFVIIGS GSAGSAMAYR LSENGRYSVI VIEYGVPDVG PLIQMPAALS FPMNMETYDW 70 80 90 100 110 120 GFSSEPEPHI GGRSLVTPRG KVLGGSSSIN GMVYVRGHAC DFDHWSQSGA RGWAYADVLP 130 140 150 160 170 180 YFKRMENSQG GQEGWRGTNG PLYVQRGKRD NPLFHAFVEA GHQAGFEVTD DYNGEKQEGF 190 200 210 220 230 240 GPMEQTIHNG RRWSAANAYL KPALKRPNVK LVKGFARKIV LEGKRAVGVE IEAGRTFSTI 250 260 270 280 290 300 RARREVIIAA SSINSPKLLM LSGIGPAAHL KEHGIDLVAD RPGVGQNLQD HLEVYIQQEC 310 320 330 340 350 360 TQPITLYSEL NLFSKARIGV EWLLFKTGDG ATNHFESAAF VRSKAGVEYP DIQYHFLPVA 370 380 390 400 410 420 IRYDGKAAAQ SHGFQAHVGP MRSKSRGSVT LRSANPREKP VIKFNYMSHE DDWADFRHCV 430 440 450 460 470 480 RLTREIFGQA AFDPYRGAEI QPGAHVQTDD EIDNFIREHV ESAFHPCGTC KMGAVDDPMA 490 500 510 520 530 540 VVDPECRVIG VEGLRVADSS IFPRITNGNL NGPSIMVGEK ASDHILGRTP LARSNQEPWI NPRWQVSDR

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014291 Genomic DNA. Translation: AAN29484.1.
RefSeq	NP_697569.1.
3D structure databases
HSSP	HSSP built from PDB template 1JU2 based on UniProtKB Q945K2.
ModBase	Search...
Genome annotation databases
GeneID	1166215.
GenomeReviews	Gene locus BR0553 in contig AE014291_GR.
KEGG	bms:BR0553.
NMPDR	fig|204722.1.peg.526.
TIGR	BR0553.
Phylogenomic databases
HOGENOM	Q8G1Z8.
OMA	PNLQYHF.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0553-MON.
BRENDA	1.1.99.1. 281610.
Family and domain databases
HAMAP	MF_00750. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_BRUSU

Accession

Primary (citable) accession number: Q8G1Z8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)