Reviewed, UniProtKB/Swiss-Prot Q8G1R0 (DAPA_BRUSU)

Names and origin

Protein names

Recommended name:
    Dihydrodipicolinate synthase
      Short name=DHDPS
    EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	BR0646

Organism

Brucella suis [Complete proteome] [HAMAP]

Taxonomic identifier

29461 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 293	293	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_0000103089
Regions
	Region	48 – 49	2	Pyruvate binding By similarity
Sites
	Active site	162	1	Schiff-base intermediate with substrate By similarity
	Binding site	106	1	Pyruvate By similarity
	Site	133	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8G1R0-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 4687B828C4AA5C7B	FASTA	293	31,598
	10 20 30 40 50 60 MLKGSITALV TPFDREGAFD EKAFRAFVNW QIEEGTKGLV PVGTTGETPT LSHDEHKRVI 70 80 90 100 110 120 EVCIEVAAGR VPVIAGAGSN NTVEAIELAQ HAEKAGADAV LVVTPYYNKP NQRGLYEHFS 130 140 150 160 170 180 RVVRSISIPL VIYNIPGRSI IDMTPETMGA LVRDCKNIVG VKDATGKIER VSEQRAICGK 190 200 210 220 230 240 EFIQLSGEDA TALGFNAHGG VGCISVTSNI APRLCAEFQE ACQAGNFAKA LELQDRLMPL 250 260 270 280 290 HKALFLEPNP SGPKYALSRL GRIENVLRSP MVTIEAATAE KIDHAMKHAG LIN

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014291 Genomic DNA. Translation: AAN29575.1.
RefSeq	NP_697660.1.
3D structure databases
HSSP	HSSP built from PDB template 1DHP based on UniProtKB P05640.
ModBase	Search...
Genome annotation databases
GeneID	1166309.
GenomeReviews	Gene locus BR0646 in contig AE014291_GR.
KEGG	bms:BR0646.
NMPDR	fig|204722.1.peg.617.
TIGR	BR0646.
Phylogenomic databases
HOGENOM	Q8G1R0.
OMA	IYNIPPR.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0646-MON.
BRENDA	4.2.1.52. 281610.
Family and domain databases
HAMAP	MF_00418. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005263. DapA_synth. IPR002220. DHDPS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PRINTS	PR00146. DHPICSNTHASE.
ProDom	PD001859. DHDPS. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00674. dapA. 1 hit.
PROSITE	PS00665. DHDPS_1. False negative. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_BRUSU

Accession

Primary (citable) accession number: Q8G1R0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)