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Q8G1R0

- DAPA_BRUSU

UniProt

Q8G1R0 - DAPA_BRUSU

Protein

4-hydroxy-tetrahydrodipicolinate synthase

Gene

dapA

Organism
Brucella suis biovar 1 (strain 1330)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).UniRule annotation

    Catalytic activityi

    Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H2O.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei44 – 441Part of a proton relay during catalysisUniRule annotation
    Binding sitei45 – 451PyruvateUniRule annotation
    Sitei107 – 1071Part of a proton relay during catalysisUniRule annotation
    Active sitei133 – 1331Proton donor/acceptorUniRule annotation
    Active sitei162 – 1621Schiff-base intermediate with substrateUniRule annotation
    Binding sitei204 – 2041Pyruvate; via carbonyl oxygenUniRule annotation

    GO - Molecular functioni

    1. 4-hydroxy-tetrahydrodipicolinate synthase Source: UniProtKB-EC
    2. amine-lyase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. diaminopimelate biosynthetic process Source: UniProtKB-HAMAP
    2. lysine biosynthetic process via diaminopimelate Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Amino-acid biosynthesis, Diaminopimelate biosynthesis, Lysine biosynthesis

    Keywords - Ligandi

    Schiff base

    Enzyme and pathway databases

    UniPathwayiUPA00034; UER00017.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxy-tetrahydrodipicolinate synthaseUniRule annotation (EC:4.3.3.7UniRule annotation)
    Short name:
    HTPA synthaseUniRule annotation
    Gene namesi
    Name:dapAUniRule annotation
    Ordered Locus Names:BR0646, BS1330_I0642
    OrganismiBrucella suis biovar 1 (strain 1330)
    Taxonomic identifieri204722 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000000824: Chromosome I, UP000007104: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2932934-hydroxy-tetrahydrodipicolinate synthasePRO_0000103089Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer; dimer of dimers.UniRule annotation

    Protein-protein interaction databases

    STRINGi204722.BR0646.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8G1R0.
    SMRiQ8G1R0. Positions 1-292.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DapA family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0329.
    HOGENOMiHOG000173604.
    KOiK01714.
    OMAiMLYSGDD.
    OrthoDBiEOG6W7235.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00418. DapA.
    InterProiIPR013785. Aldolase_TIM.
    IPR005263. DapA.
    IPR002220. DapA-like.
    IPR020625. Dihydrodipicolinate_synth_AS.
    [Graphical view]
    PANTHERiPTHR12128. PTHR12128. 1 hit.
    PfamiPF00701. DHDPS. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001365. DHDPS. 1 hit.
    PRINTSiPR00146. DHPICSNTHASE.
    TIGRFAMsiTIGR00674. dapA. 1 hit.
    PROSITEiPS00666. DHDPS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8G1R0-1 [UniParc]FASTAAdd to Basket

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    MLKGSITALV TPFDREGAFD EKAFRAFVNW QIEEGTKGLV PVGTTGETPT    50
    LSHDEHKRVI EVCIEVAAGR VPVIAGAGSN NTVEAIELAQ HAEKAGADAV 100
    LVVTPYYNKP NQRGLYEHFS RVVRSISIPL VIYNIPGRSI IDMTPETMGA 150
    LVRDCKNIVG VKDATGKIER VSEQRAICGK EFIQLSGEDA TALGFNAHGG 200
    VGCISVTSNI APRLCAEFQE ACQAGNFAKA LELQDRLMPL HKALFLEPNP 250
    SGPKYALSRL GRIENVLRSP MVTIEAATAE KIDHAMKHAG LIN 293
    Length:293
    Mass (Da):31,598
    Last modified:March 1, 2003 - v1
    Checksum:i4687B828C4AA5C7B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014291 Genomic DNA. Translation: AAN29575.1.
    CP002997 Genomic DNA. Translation: AEM17992.1.
    RefSeqiNP_697660.1. NC_004310.3.
    YP_005615484.1. NC_017251.1.

    Genome annotation databases

    EnsemblBacteriaiAAN29575; AAN29575; BR0646.
    AEM17992; AEM17992; BS1330_I0642.
    GeneIDi1166309.
    12136963.
    KEGGibms:BR0646.
    bsi:BS1330_I0642.
    PATRICi17789587. VBIBruSui107850_0660.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014291 Genomic DNA. Translation: AAN29575.1 .
    CP002997 Genomic DNA. Translation: AEM17992.1 .
    RefSeqi NP_697660.1. NC_004310.3.
    YP_005615484.1. NC_017251.1.

    3D structure databases

    ProteinModelPortali Q8G1R0.
    SMRi Q8G1R0. Positions 1-292.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 204722.BR0646.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN29575 ; AAN29575 ; BR0646 .
    AEM17992 ; AEM17992 ; BS1330_I0642 .
    GeneIDi 1166309.
    12136963.
    KEGGi bms:BR0646.
    bsi:BS1330_I0642.
    PATRICi 17789587. VBIBruSui107850_0660.

    Phylogenomic databases

    eggNOGi COG0329.
    HOGENOMi HOG000173604.
    KOi K01714.
    OMAi MLYSGDD.
    OrthoDBi EOG6W7235.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00017 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00418. DapA.
    InterProi IPR013785. Aldolase_TIM.
    IPR005263. DapA.
    IPR002220. DapA-like.
    IPR020625. Dihydrodipicolinate_synth_AS.
    [Graphical view ]
    PANTHERi PTHR12128. PTHR12128. 1 hit.
    Pfami PF00701. DHDPS. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001365. DHDPS. 1 hit.
    PRINTSi PR00146. DHPICSNTHASE.
    TIGRFAMsi TIGR00674. dapA. 1 hit.
    PROSITEi PS00666. DHDPS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1330.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1330.

    Entry informationi

    Entry nameiDAPA_BRUSU
    AccessioniPrimary (citable) accession number: Q8G1R0
    Secondary accession number(s): G0K7Y7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 23, 2003
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.Curated

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Brucella suis
      Brucella suis (strain 1330): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3