Q8G1M4 (AMPA_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cytosol aminopeptidase
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	BR0689, BS1330_I0685

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	497 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 497	497	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_0000165729
Sites
	Active site	275	1	Potential
	Active site	349	1	Potential
	Metal binding	263	1	Manganese 2 By similarity
	Metal binding	268	1	Manganese 1 By similarity
	Metal binding	268	1	Manganese 2 By similarity
	Metal binding	286	1	Manganese 2 By similarity
	Metal binding	345	1	Manganese 1 By similarity
	Metal binding	347	1	Manganese 1 By similarity
	Metal binding	347	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8G1M4 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 287DD9E0C90DC3AC	FASTA	497	52,576
	10 20 30 40 50 60 MSKRPSISFS EFEVPQKGVA VVLVAKGGGF ADEAAKAVGG AEKIARIADI SGFTGALGKT 70 80 90 100 110 120 AEAIETTPAG VEKIVLVGVG EPGKLGNDDW LKIGGAAFSQ IGNAERVTLT LALPETTIAG 130 140 150 160 170 180 DEAADVALGM VLRSYKFDRY KTRKSEENGE PKHAAKITIC VADTHSARKT FEVAEAVADG 190 200 210 220 230 240 VIQARNLVNE PANILGPVEF AEEAEKLEKL GVKVEVLGEK ELKKLGMGAL LGVAQGSVRP 250 260 270 280 290 300 PRLVVMEWHG AKGKEKPIAF VGKGVVFDTG GISIKPAANM EDMKGDMGGA AAVTGLMRAL 310 320 330 340 350 360 AGRKAKVNAI GVIGLVENMP DGNAQRPGDI VTSMSGQTIE VINTDAEGRL VLADALHYTN 370 380 390 400 410 420 DRFKPRFIIN LATLTGAVMV ALGQYHAGLF SNDDELADQL YDAGQSTGEK LWRLPLGTEY 430 440 450 460 470 480 DKMIDSKFAD MKNSAGRYGG SITAAQFLKR FVGETPWAHL DVAGTAMGSP ANEYNQSWAS 490 GFGVRLLDRL VRDQFES

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed: 22038969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN29618.1. CP002997 Genomic DNA. Translation: AEM18035.1.
RefSeq	NP_697703.1. NC_004310.3.
3D structure databases
ProteinModelPortal	Q8G1M4.
ModBase	Search...
Protein family/group databases
MEROPS	M17.003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1166352.
GenomeReviews	Gene locus BR0689 in contig AE014291_GR.
KEGG	bms:BR0689.
NMPDR	fig|204722.1.peg.660.
PATRIC	17789669. VBIBruSui107850_0701.
TIGR	BR0689.
Phylogenomic databases
HOGENOM	HBG742580.
OMA	NMHLMRY.
PhylomeDB	Q8G1M4.
ProtClustDB	PRK00913.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0689-MONOMER.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
KO	K01255.
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_BRUSU

Accession

Primary (citable) accession number: Q8G1M4
Secondary accession number(s): G0K830

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program