Reviewed, UniProtKB/Swiss-Prot Q8G1E8 (RISB1_BRUSU)

Names and origin

Protein names

Recommended name:
    6,7-dimethyl-8-ribityllumazine synthase 1
      Short name=DMRL synthase 1
      Short name=Lumazine synthase 1
    EC=2.5.1.9
Alternative name(s):
    Riboflavin synthase 1 beta chain

Gene names

Name:	ribH1
Synonyms:	ribH-1
Ordered Locus Names:	BR0769

Organism

Brucella suis [Complete proteome] [HAMAP]

Taxonomic identifier

29461 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	157 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate yielding 6,7-dimethyl-8-lumazine By similarity.
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine. HAMAP MF_00178
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil and riboflavin from 6,7-dimethyl-8-(1-D-ribityl)lumazine: step 1/1. HAMAP MF_00178
Sequence similarities	Belongs to the DMRL synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	riboflavin synthase complex Inferred from electronic annotation. Source: InterPro
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 157	157	6,7-dimethyl-8-ribityllumazine synthase 1 HAMAP MF_00178		PRO_0000134728

Sequences

	Sequence		Length	Mass (Da)
	Q8G1E8-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 657C4A1FE82EB264	FASTA	157	16,796
	10 20 30 40 50 60 MDFLMSKHEA DAPHLLIVEA RFYDDLADAL LDGAKAALDE AGATYDVVTV PGALEIPATI 70 80 90 100 110 120 SFALDGADNG GTEYDGFVAL GTVIRGETYH FDIVSNESCR ALTDLSVEES IAIGNGILTV 130 140 150 ENEEQAWVRA RREDKDKGGF AARAALTMIG LRKKFGA

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014291 Genomic DNA. Translation: AAN29698.1.
RefSeq	NP_697783.1.
3D structure databases
HSSP	HSSP built from PDB template 1RVV based on UniProtKB P11998.
SMR	Q8G1E8. Positions 11-157.
ModBase	Search...
Genome annotation databases
GeneID	1166434.
GenomeReviews	Gene locus BR0769 in contig AE014291_GR.
KEGG	bms:BR0769.
NMPDR	fig|204722.1.peg.740.
TIGR	BR0769.
Phylogenomic databases
HOGENOM	Q8G1E8.
OMA	TIHFEIV.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0769-MON.
BRENDA	2.5.1.9. 281610.
Family and domain databases
HAMAP	MF_00178. [Tree]
InterPro	IPR002180. DMRL_synthase. [Graphical view]
Gene3D	G3DSA:3.40.50.960. DMRL_synthase. 1 hit.
PANTHER	PTHR21058. DMRL_synthase. 1 hit.
Pfam	PF00885. DMRL_synthase. 1 hit. [Graphical view]
ProDom	PD003664. DMRL_synthase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00114. lumazine-synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RISB1_BRUSU

Accession

Primary (citable) accession number: Q8G1E8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)