Q8G1A7 (NUOK_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit K
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit K
NDH-1 subunit K

Gene names

Name:	nuoK
Ordered Locus Names:	BR0812, BS1330_I0808

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella suis

Protein attributes

Sequence length	102 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP-Rule MF_01456
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP-Rule MF_01456
Subunit structure	NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_01456.
Sequence similarities	Belongs to the complex I subunit 4L family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro photosynthesis, light reaction Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	NADH dehydrogenase (quinone) activity Inferred from electronic annotation. Source: HAMAP quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 102	102	NADH-quinone oxidoreductase subunit K HAMAP-Rule MF_01456		PRO_0000389977
Regions
	Transmembrane	5 – 25	21	Helical; Potential
	Transmembrane	31 – 51	21	Helical; Potential
	Transmembrane	66 – 86	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8G1A7 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: E3EC572231BD3204	FASTA	102	10,942
	10 20 30 40 50 60 MEIGIAHYLT VSAILFTLGV FGIFLNRKNV IVILMSIELI LLSVNLNFVA FSSQLGDLVG 70 80 90 100 QVFALFVLTV AAAEAAIGLA ILVVFFRNRG SIAVEDVNVM KG

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN29741.1. CP002997 Genomic DNA. Translation: AEM18158.1.
PIR	AF3395.
RefSeq	NP_697826.1. NC_004310.3. YP_005615650.1. NC_017251.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	204722.BR0812.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN29741; AAN29741; BR0812. AEM18158; AEM18158; BS1330_I0808.
GeneID	1166480. 12137134.
KEGG	bms:BR0812. bsi:BS1330_I0808.
PATRIC	17789922. VBIBruSui107850_0822.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0713.
HOGENOM	HOG000066429.
KO	K00340.
OMA	RVTVDME.
ProtClustDB	PRK05715.
Family and domain databases
HAMAP	MF_01456. NDH1_NuoK.
InterPro	IPR001133. NADH_UbQ_OxRdtase_chain4L/K. [Graphical view]
PANTHER	PTHR11434. PTHR11434. 1 hit.
Pfam	PF00420. Oxidored_q2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOK_BRUSU

Accession

Primary (citable) accession number: Q8G1A7
Secondary accession number(s): G0K8W0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2009
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program