Q8G197 (SYP_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	BR0822, BS1330_I0818

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella suis

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP-Rule MF_01570
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP-Rule MF_01570
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 442	442	Proline--tRNA ligase HAMAP-Rule MF_01570		PRO_0000248895

Sequences

	Sequence		Length	Mass (Da)
	Q8G197 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 824BD1F8BB4875B1	FASTA	442	49,450
	10 20 30 40 50 60 MRLSRYFLPI LKENPKEAEI VSHRLMLRSG MIRQQSAGIY SWLPIGLKVL NKVCTIIREE 70 80 90 100 110 120 QNRAGANEIL MPTIQSADLW RESGRYGAYG KEMLRIQDRQ EREMLFGPTN EEMVTDIFRS 130 140 150 160 170 180 YVRSYKDLPL NLYHIQWKFR DEVRPRFGVM RSREFLMKDA YSFDLDYEGA KMAYYRMFVS 190 200 210 220 230 240 YLRTFARVGL QAIPMRADTG PIGGDLSHEF IILAETGESQ VYCDRAYLDL AVPGADTDFR 250 260 270 280 290 300 NDAQLTDIVT RWTTPYAATD EMHDEADWAK VKPESQVSAR GIEVGHIFHF GTKYSEPMGA 310 320 330 340 350 360 KVQGPDGKEH LVSMGSYGIG PSRLVAAAIE ASHDDAGIIW PKAIAPFGAG IVNMKPGDEG 370 380 390 400 410 420 CDGVSEKLYE ALTNAGVDPL LDDKDERPGA KFATMDLIGL PTQVIVGPRG VAAGEVEVKD 430 440 RKMGERQSLG IEAAINMLTA QA

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN29751.1. CP002997 Genomic DNA. Translation: AEM18168.1.
RefSeq	NP_697836.1. NC_004310.3. YP_005615660.1. NC_017251.1.
3D structure databases
ProteinModelPortal	Q8G197.
SMR	Q8G197. Positions 1-439.
ModBase	Search...
Protein-protein interaction databases
STRING	204722.BR0822.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN29751; AAN29751; BR0822. AEM18168; AEM18168; BS1330_I0818.
GeneID	1166490. 12137144.
KEGG	bms:BR0822. bsi:BS1330_I0818.
PATRIC	17789944. VBIBruSui107850_0833.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0442.
HOGENOM	HOG000076894.
KO	K01881.
OMA	IQPAELW.
ProtClustDB	PRK12325.
Family and domain databases
Gene3D	3.40.50.800. 1 hit.
HAMAP	MF_01570. Pro_tRNA_synth_type2.
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-ligase_IIa. IPR004500. Pro-tRNA-synth_IIa_bac-type. IPR023716. Prolyl-tRNA_ligase_IIa_type2. [Graphical view]
PANTHER	PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit.
TIGRFAMs	TIGR00409. proS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_BRUSU

Accession

Primary (citable) accession number: Q8G197
Secondary accession number(s): G0K8X0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program