Q8G189 (TTCA_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA 2-thiocytidine biosynthesis protein TtcA

Gene names

Name:	ttcA
Ordered Locus Names:	BR0831, BS1330_I0827

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella suis

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s2C32) By similarity. HAMAP-Rule MF_01850
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01850.
Sequence similarities	Belongs to the TtcA family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 293	293	tRNA 2-thiocytidine biosynthesis protein TtcA HAMAP-Rule MF_01850		PRO_0000348677
Regions
	Nucleotide binding	62 – 67	6	ATP Potential
	Motif	137 – 140	4	CXXC HAMAP-Rule MF_01850
	Motif	225 – 228	4	CXXC HAMAP-Rule MF_01850

Sequences

	Sequence		Length	Mass (Da)
	Q8G189 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 39AA1E5ECF5898F7	FASTA	293	33,259
	10 20 30 40 50 60 MNAFDADITE HADSSGCHPL FRDVPATVEF NKLRKRLLRL TRQAIEDFAM VKPGDRWMVC 70 80 90 100 110 120 LSGGKDSYGL LALLLDLKWR GLLPVELLAV NLDQGQPNFP KHILPDFLTR YGIEHRIEYQ 130 140 150 160 170 180 DTYSIVTDKL PETSTYCSLC SRLRRGNLYR IAREEGCSAI VLGHHREDIL ETFFMNLFHG 190 200 210 220 230 240 GRLAAMPPKL LNDEGDLMVF RPLAYAAEDD LEKFANAMQF PIIPCDLCGS QDGLQRNAMK 250 260 270 280 290 AMLIDIEKRM PGRKDTMIRA LTNVRPSHLL DRKLFDFAGL MANGEKGSDD ALW

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN29760.1. CP002997 Genomic DNA. Translation: AEM18177.1.
RefSeq	NP_697845.1. NC_004310.3. YP_005615669.1. NC_017251.1.
3D structure databases
ProteinModelPortal	Q8G189.
ModBase	Search...
Protein-protein interaction databases
STRING	204722.BR0831.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN29760; AAN29760; BR0831. AEM18177; AEM18177; BS1330_I0827.
GeneID	1166499. 12137153.
KEGG	bms:BR0831. bsi:BS1330_I0827.
PATRIC	17789964. VBIBruSui107850_0843.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0037.
HOGENOM	HOG000013323.
KO	K14058.
OMA	EHILPEY.
ProtClustDB	PRK10696.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_01850. TtcA.
InterPro	IPR012089. 2-thiocytidine_tRNA_synth_TtcA. IPR014729. Rossmann-like_a/b/a_fold. IPR011063. tRNA-lysidine/thiocyt_synth. [Graphical view]
Pfam	PF01171. ATP_bind_3. 1 hit. [Graphical view]
PIRSF	PIRSF004976. ATPase_YdaO. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TTCA_BRUSU

Accession

Primary (citable) accession number: Q8G189
Secondary accession number(s): G0K8X9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program