Q8G180 (PURQ_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 1
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase I
Short name=FGAM synthase I

Gene names

Name:	purQ
Ordered Locus Names:	BR0840, BS1330_I0836

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella suis

Protein attributes

Sequence length	223 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP-Rule MF_00421
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP-Rule MF_00421
Subunit structure	Heterodimer of two subunits, PurQ and PurL By similarity.
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00421.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway glutamine metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 223	223	Phosphoribosylformylglycinamidine synthase 1 HAMAP-Rule MF_00421		PRO_0000100545
Regions
	Domain	3 – 223	221	Glutamine amidotransferase type-1
Sites
	Active site	87	1	Nucleophile By similarity
	Active site	197	1	By similarity
	Active site	199	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8G180 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: C548E2FAAB14EF67	FASTA	223	23,768
	10 20 30 40 50 60 MKSAVILLPG LNRDRDMIAA LTKITGQAPV TVWQTDTSIP DDVDLILIPG GFSYGDYLRC 70 80 90 100 110 120 GAIAARMPVM QAVREKADKG VMVMGVCNGF QILLEAGLLP GALMRNASLK FVCREVKLEV 130 140 150 160 170 180 TNANTSFTRG YKPGQIIRCP VAHHDGNYFA DAETLKRLEG EGQVVFRYAE GTNPNGSVND 190 200 210 220 IAGIVNARGN VLGMMPHPEN LIEAAHGGDD GRALFAGALG ITA

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN29769.1. CP002997 Genomic DNA. Translation: AEM18186.1.
RefSeq	NP_697854.1. NC_004310.3. YP_005615678.1. NC_017251.1.
3D structure databases
ProteinModelPortal	Q8G180.
ModBase	Search...
Protein-protein interaction databases
STRING	204722.BR0840.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN29769; AAN29769; BR0840. AEM18186; AEM18186; BS1330_I0836.
GeneID	1166508. 12137162.
KEGG	bms:BR0840. bsi:BS1330_I0836.
PATRIC	17789980. VBIBruSui107850_0851.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0047.
HOGENOM	HOG000238240.
KO	K01952.
OMA	VLYRYCS.
ProtClustDB	PRK03619.
Enzyme and pathway databases
UniPathway	UPA00074; UER00128.
Family and domain databases
HAMAP	MF_00421. PurQ.
InterPro	IPR017926. GATASE. IPR010075. PRibForGlyAmidine_synth_I. [Graphical view]
PIRSF	PIRSF001586. FGAM_synth_I. 1 hit.
TIGRFAMs	TIGR01737. FGAM_synth_I. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURQ_BRUSU

Accession

Primary (citable) accession number: Q8G180
Secondary accession number(s): G0K8Y8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 7, 2003
Last sequence update:	March 1, 2003
Last modified:	May 29, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program