Q8G0Z4 (PPNK_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable inorganic polyphosphate/ATP-NAD kinase
Short name=Poly(P)/ATP NAD kinase
EC=2.7.1.23

Gene names

Name:	ppnK
Ordered Locus Names:	BR0937, BS1330_I0933

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella suis

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus By similarity. HAMAP-Rule MF_00361
Catalytic activity	ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361
Cofactor	Divalent metal ions By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NAD kinase family.
Sequence caution	The sequence AAN29863.1 differs from that shown. Reason: Erroneous initiation. The sequence AEM18280.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding NAD NADP Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	NAD metabolic process Inferred from electronic annotation. Source: InterPro NADP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ kinase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 257	257	Probable inorganic polyphosphate/ATP-NAD kinase HAMAP-Rule MF_00361		PRO_0000120604

Sequences

	Sequence		Length	Mass (Da)
	Q8G0Z4 [UniParc]. Last modified November 7, 2003. Version 2. Checksum: 54ACA8EF3504F74A	FASTA	257	28,275
	10 20 30 40 50 60 MKDTSLALHF VSSGTKESLS AQKDLVERYG HVAAEDADII VALGGDGTML QALRDFMNTG 70 80 90 100 110 120 KPIYGMNRGS VGFLMNEFVI ENLPERILAA QMETIRPLVM VAETEDAPPV EALAINEVSL 130 140 150 160 170 180 FRQSYQAARI RITIDGKVRL QELVCDGVMV ATPAGSTAYN LSAQGPILPL EAPLLALTPV 190 200 210 220 230 240 SPFRPRRWGG ALLPKHVTVR MDLLETEKRP VNAVADNNEV KSVTSVTVRE APNSQVTILF 250 DKNHSWDERI LTEQFRH

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN29863.1. Different initiation. CP002997 Genomic DNA. Translation: AEM18280.1. Different initiation.
RefSeq	NP_697948.1. NC_004310.3. YP_005615772.1. NC_017251.1.
3D structure databases
ProteinModelPortal	Q8G0Z4.
ModBase	Search...
Protein-protein interaction databases
STRING	204722.BR0937.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN29863; AAN29863; BR0937. AEM18280; AEM18280; BS1330_I0933.
GeneID	1166607. 12137960.
KEGG	bms:BR0937. bsi:BS1330_I0933.
PATRIC	17790186. VBIBruSui107850_0952.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0061.
HOGENOM	HOG000139847.
KO	K00858.
ProtClustDB	PRK04761.
Family and domain databases
Gene3D	2.60.200.30. 1 hit. 3.40.50.10330. 1 hit.
HAMAP	MF_00361. NAD_kinase.
InterPro	IPR017438. ATP-NAD_kinase_dom_1. IPR016064. ATP-NAD_kinase_PpnK-typ. IPR017437. ATP-NAD_kinase_PpnK-typ_all-b. IPR002504. PolyP/ATP_NADK_prd. [Graphical view]
PANTHER	PTHR20275. PTHR20275. 1 hit.
Pfam	PF01513. NAD_kinase. 1 hit. [Graphical view]
SUPFAM	SSF111331. ATP-NAD_kinase_PpnK-typ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPNK_BRUSU

Accession

Primary (citable) accession number: Q8G0Z4
Secondary accession number(s): G0K9L4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 7, 2003
Last sequence update:	November 7, 2003
Last modified:	May 1, 2013
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program