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Protein

NAD kinase

Gene

nadK

Organism
Brucella suis biovar 1 (strain 1330)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei46 – 461Proton acceptorUniRule annotation
Binding sitei146 – 1461NADUniRule annotation
Binding sitei154 – 1541NAD; via carbonyl oxygenUniRule annotation
Binding sitei218 – 2181NADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi46 – 472NADUniRule annotation
Nucleotide bindingi116 – 1172NADUniRule annotation
Nucleotide bindingi157 – 1626NADUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD+ kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:BR0937, BS1330_I0933
OrganismiBrucella suis biovar 1 (strain 1330)
Taxonomic identifieri204722 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000007104 Componenti: Chromosome I UP000000824 Componenti: Chromosome I

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 257257NAD kinasePRO_0000120604Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi204722.BR0937.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000139847.
KOiK00858.
OrthoDBiEOG6PZXDR.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8G0Z4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDTSLALHF VSSGTKESLS AQKDLVERYG HVAAEDADII VALGGDGTML
60 70 80 90 100
QALRDFMNTG KPIYGMNRGS VGFLMNEFVI ENLPERILAA QMETIRPLVM
110 120 130 140 150
VAETEDAPPV EALAINEVSL FRQSYQAARI RITIDGKVRL QELVCDGVMV
160 170 180 190 200
ATPAGSTAYN LSAQGPILPL EAPLLALTPV SPFRPRRWGG ALLPKHVTVR
210 220 230 240 250
MDLLETEKRP VNAVADNNEV KSVTSVTVRE APNSQVTILF DKNHSWDERI

LTEQFRH
Length:257
Mass (Da):28,275
Last modified:November 7, 2003 - v2
Checksum:i54ACA8EF3504F74A
GO

Sequence cautioni

The sequence AAN29863.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AEM18280.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN29863.1. Different initiation.
CP002997 Genomic DNA. Translation: AEM18280.1. Different initiation.
RefSeqiNP_697948.1. NC_004310.3.
YP_005615772.1. NC_017251.1.

Genome annotation databases

EnsemblBacteriaiAAN29863; AAN29863; BR0937.
AEM18280; AEM18280; BS1330_I0933.
KEGGibms:BR0937.
bsi:BS1330_I0933.
PATRICi17790186. VBIBruSui107850_0952.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN29863.1. Different initiation.
CP002997 Genomic DNA. Translation: AEM18280.1. Different initiation.
RefSeqiNP_697948.1. NC_004310.3.
YP_005615772.1. NC_017251.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi204722.BR0937.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN29863; AAN29863; BR0937.
AEM18280; AEM18280; BS1330_I0933.
KEGGibms:BR0937.
bsi:BS1330_I0933.
PATRICi17790186. VBIBruSui107850_0952.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000139847.
KOiK00858.
OrthoDBiEOG6PZXDR.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1330.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1330.

Entry informationi

Entry nameiNADK_BRUSU
AccessioniPrimary (citable) accession number: Q8G0Z4
Secondary accession number(s): G0K9L4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: November 7, 2003
Last modified: April 1, 2015
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella suis
    Brucella suis (strain 1330): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.