Q8G0Y6 (BEPC_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Outer membrane efflux protein BepC

Gene names

Name:	bepC
Ordered Locus Names:	BR0945, BS1330_I0941

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella suis

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the efflux of toxic and relatively hydrophobic compounds. Influences survival inside the host. Ref.3
Subunit structure	Probably part of a tripartite efflux pump, which is composed of an outer membrane efflux protein, an inner membrane protein and a protein that expands the periplasmic space. Could form a tripartite pump with BepD and BepE or with BepF and BepG. Ref.3 Ref.4
Subcellular location	Cell outer membrane Probable.
Disruption phenotype	Mutation strongly affects resistance to bile salts and toxic chemicals, and significantly decreases the resistance to antibiotics such as erythromycin, ampicillin, tetracycline, and norfloxacin. The mutant also has attenuated effects in the mouse model of infection. Ref.3
Sequence similarities	Belongs to the outer membrane factor (OMF) (TC 1.B.17) family. [View classification]

Ontologies

Keywords
Biological process	Antibiotic resistance Transport
Cellular component	Cell membrane Cell outer membrane Membrane
Domain	Coiled coil Signal Transmembrane Transmembrane beta strand
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein transport Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 456	428	Outer membrane efflux protein BepC		PRO_0000390647
Regions
	Coiled coil	312 – 341	30	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8G0Y6 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 3CF3CBED5639292B	FASTA	456	48,518
	10 20 30 40 50 60 MRYTVFKACK ELVAAAVLLS GTVLTGQAAL SETLTGALVK AYKNNAPLNS SRAGVRIQDE 70 80 90 100 110 120 NVAIAKSAYR PQITGSYNIS RGKTPATDYR TTGTVGIQLN QMLFDGFQTR NNVAAAETQV 130 140 150 160 170 180 FAQRENLRND EQNTLYQAVA AYMDVYQLRQ IAALREKNLA AMNEQVRAAR ARLDVGEGTR 190 200 210 220 230 240 TDVAQAEASR STAIAALNAA RADVKTAEAT YMQVVGSLPD KLTPASAARH LPQSPSQAYA 250 260 270 280 290 300 SALASHPGIL ATKYAVNAAG YNVKAKEGAL LPTIGLTASA SQLDTIAGTD MGDGNTASIG 310 320 330 340 350 360 VGVNIPIYTG GRTSAQIRQS KEQLGQARIE VDVVQDKVRQ AISSAWSQLE AARASVAANR 370 380 390 400 410 420 DGIAAAQLAL DGVIEERKVG QRTTLDVLNA QNDLVAVQIA LVQAEHDVVV ASYALLNATG 430 440 450 RMTADQLGLQ VAQYKPEEHY KAVKDKWFGL RTPDGR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.
[2]	"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.
[3]	"The TolC homologue of Brucella suis is involved in resistance to antimicrobial compounds and virulence." Posadas D.M., Martin F.A., Sabio y Garcia J.V., Spera J.M., Delpino M.V., Baldi P., Campos E., Cravero S.L., Zorreguieta A. Infect. Immun. 75:379-389(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT, DISRUPTION PHENOTYPE. Strain: 1330.
[4]	"Interplay between two RND systems mediating antimicrobial resistance in Brucella suis." Martin F.A., Posadas D.M., Carrica M.C., Cravero S.L., O'Callaghan D., Zorreguieta A. J. Bacteriol. 191:2530-2540(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN29871.1. CP002997 Genomic DNA. Translation: AEM18288.1.
RefSeq	NP_697956.1. NC_004310.3. YP_005615780.1. NC_017251.1.
3D structure databases
ProteinModelPortal	Q8G0Y6.
ModBase	Search...
Protein-protein interaction databases
STRING	204722.BR0945.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN29871; AAN29871; BR0945. AEM18288; AEM18288; BS1330_I0941.
GeneID	1166615. 12137968.
KEGG	bms:BR0945. bsi:BS1330_I0941.
PATRIC	17790200. VBIBruSui107850_0959.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1538.
HOGENOM	HOG000143761.
KO	K12340.
OMA	NIELPRI.
ProtClustDB	CLSK863170.
Family and domain databases
InterPro	IPR003423. OMP_efflux. IPR010130. T1SS_OMP_TolC. [Graphical view]
Pfam	PF02321. OEP. 2 hits. [Graphical view]
TIGRFAMs	TIGR01844. type_I_sec_TolC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BEPC_BRUSU

Accession

Primary (citable) accession number: Q8G0Y6
Secondary accession number(s): G0K9M2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 19, 2010
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program