Q8G0P3 (XGPT_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Xanthine phosphoribosyltransferase
EC=2.4.2.22

Alternative name(s):
Xanthine-guanine phosphoribosyltransferase
Short name=XGPRT

Gene names

Name:	gpt
Ordered Locus Names:	BR1046, BS1330_I1042

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella suis

Protein attributes

Sequence length	165 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Acts on guanine, xanthine and to a lesser extent hypoxanthine By similarity. HAMAP-Rule MF_01903
Catalytic activity	XMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + xanthine. HAMAP-Rule MF_01903
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_01903
Pathway	Purine metabolism; XMP biosynthesis via salvage pathway; XMP from xanthine: step 1/1. HAMAP-Rule MF_01903
Subunit structure	Homotetramer By similarity. HAMAP-Rule MF_01903
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity HAMAP-Rule MF_01903.
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. XGPT subfamily.

Ontologies

Keywords
Biological process	Purine salvage
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	Magnesium Metal-binding
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	XMP salvage Inferred from electronic annotation. Source: UniProtKB-UniPathway purine ribonucleoside salvage Inferred from electronic annotation. Source: HAMAP
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP xanthine phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 165	165	Xanthine phosphoribosyltransferase HAMAP-Rule MF_01903		PRO_0000139659
Regions
	Region	41 – 42	2	5-phosphoribose 1-diphosphate binding By similarity
	Region	102 – 106	5	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Metal binding	99	1	Magnesium By similarity
	Binding site	102	1	Xanthine By similarity
	Binding site	145	1	Xanthine; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8G0P3 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 5B8B870CE853046E	FASTA	165	18,370
	10 20 30 40 50 60 MSLPDKAFPV SWDQFHRDAR ALAWRIAGLD REWRAIVAIT RGGLVPAAII CRELGIRLIE 70 80 90 100 110 120 TVCIASYHDY TSQGEMQVLK GIGASLLENQ GEGVIVVDDL TDTGKTAAIV REMMPRAHFA 130 140 150 160 TVYAKPKGRP LIDTFVTEVS QDTWIYFPWD MGFTYQEPIA GGKRG

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN29966.1. CP002997 Genomic DNA. Translation: AEM18384.1.
RefSeq	NP_698051.1. NC_004310.3. YP_005615876.1. NC_017251.1.
3D structure databases
ProteinModelPortal	Q8G0P3.
SMR	Q8G0P3. Positions 6-160.
ModBase	Search...
Protein-protein interaction databases
STRING	204722.BR1046.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN29966; AAN29966; BR1046. AEM18384; AEM18384; BS1330_I1042.
GeneID	1166719. 12138072.
KEGG	bms:BR1046. bsi:BS1330_I1042.
PATRIC	17790406. VBIBruSui107850_1059.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0503.
HOGENOM	HOG000226805.
KO	K00769.
OMA	PKAHFAT.
ProtClustDB	PRK09177.
Enzyme and pathway databases
UniPathway	UPA00602; UER00658.
Family and domain databases
HAMAP	MF_01903. XGPRT.
InterPro	IPR000836. PRibTrfase_dom. IPR023747. Xanthine_Guanine_PRibTrfase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XGPT_BRUSU

Accession

Primary (citable) accession number: Q8G0P3
Secondary accession number(s): G0K9W8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program