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Q8G0L8 (SYT_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Threonine--tRNA ligase
EC=6.1.1.3
Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS
Gene names
Name:thrS
Ordered Locus Names:BR1071, BS1330_I1067
OrganismBrucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]
Taxonomic identifier204722 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length658 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00184

Subunit structure

Homodimer By similarity. HAMAP MF_00184

Subcellular location

Cytoplasm HAMAP MF_00184.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processthreonyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

threonine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 658658Threonine--tRNA ligase HAMAP MF_00184
PRO_0000100949

Regions

Region246 – 548303Catalytic HAMAP MF_00184

Sites

Metal binding3431Zinc; catalytic By similarity
Metal binding3941Zinc; catalytic By similarity
Metal binding5251Zinc; catalytic By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8G0L8 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: EA353A28C1C5E3DB

FASTA65874,578
        10         20         30         40         50         60 
MSNTVSLQFP DGSVREYDAS MTGAALAESI SKSLAKKAVA YAVDGTVRDL SDPLGASGKV 

        70         80         90        100        110        120 
EIITREDPRA LELIRHDTAH VLAEAVQELF PGTQVTIGPV IENGFYYDFA RNEPFTLDDL 

       130        140        150        160        170        180 
PVIEKKMREI IQRNKPFTKE VWSREKAKQV FSDKGESYKV ELVDAIPAGQ DLKIYYQGDW 

       190        200        210        220        230        240 
FDLCRGPHMA STGQIGNSFK LMKVAGAYWR GDANNPMLTR IYGTAFANDN DLQAYLHMLE 

       250        260        270        280        290        300 
EAEKRDHRRL GREMDLFHFQ EEGPGVVFWH AKGWKMFQNL VSYMRRRLDS HGYQEVNAPQ 

       310        320        330        340        350        360 
VLDKSLWETS GHWGWYRDNM FKVTVAGDDT DDDRVFALKP MNCPGHVQIF KHGLKSYRDL 

       370        380        390        400        410        420 
PIKLAEFGNV HRYEPSGALH GLMRVRGFTQ DDAHIFCTEE QMAAECLRIN DLILSVYKDF 

       430        440        450        460        470        480 
GFEEITIKLS TRPEKRVGSD ELWDRAESVM MTVLEQIRQQ SNNIKTGILP GEGAFYGPKF 

       490        500        510        520        530        540 
EYTLKDAIGR EWQCGTTQVD FNLPERFGAF YIGADSEKKQ PVMIHRAICG SMERFLGILI 

       550        560        570        580        590        600 
ENFAGHMPLW FAPVQVVVAT ITSDADEYAK EAAAKLKAAG LQVVTDLRNE KINYKVREHS 

       610        620        630        640        650 
LQKVPVILVC GKREAEEKTV NMRRLGSRDQ ESMTLDEAIA RLCEEATPPD LLRLKNAG

« Hide

References

[1]"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts."
Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. expand/collapse author list , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.
[2]"Revised genome sequence of Brucella suis 1330."
Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.
J. Bacteriol. 193:6410-6410(2011) [PubMed: 22038969] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE014291 Genomic DNA. Translation: AAN29991.1.
CP002997 Genomic DNA. Translation: AEM18409.1.
RefSeqNP_698076.1. NC_004310.3.

3D structure databases

ProteinModelPortalQ8G0L8.
SMRQ8G0L8. Positions 245-646.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1166745.
GenomeReviewsGene locus BR1071 in contig AE014291_GR.
KEGGbms:BR1071.
NMPDRfig|204722.1.peg.1033.
PATRIC17790460. VBIBruSui107850_1085.
TIGRBR1071.

Phylogenomic databases

HOGENOMHBG352811.
OMAVGSDALW.
PhylomeDBQ8G0L8.
ProtClustDBPRK00413.

Enzyme and pathway databases

BioCycBSUI204722:BR_1071-MONOMER.

Family and domain databases

HAMAPMF_00184. Thr_tRNA_synth.
[Tree]
InterProIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR012675. Beta-grasp_ferredoxin-type.
IPR012676. TGS-like.
IPR002320. Thr-tRNA-synth_IIa.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
Gene3DG3DSA:3.40.50.800. Anticodon_bd. 1 hit.
G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
KOK01868.
PfamPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSPR01047. TRNASYNTHTHR.
SMARTSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMSSF52954. Anticodon_bd. 1 hit.
SSF81271. TGS-like. 1 hit.
SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMsTIGR00418. ThrS. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYT_BRUSU
AccessionPrimary (citable) accession number: Q8G0L8
Secondary accession number(s): G0K9Z3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: March 1, 2003
Last modified: January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

Brucella suis

Brucella suis (strain 1330): entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents