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Protein

Lon protease

Gene

lon

Organism
Brucella suis biovar 1 (strain 1330)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner.UniRule annotation

Catalytic activityi

Hydrolysis of proteins in presence of ATP.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei689 – 6891UniRule annotation
Active sitei732 – 7321UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi367 – 3748ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. ATP-dependent peptidase activity Source: UniProtKB-HAMAP
  3. sequence-specific DNA binding Source: UniProtKB-HAMAP
  4. serine-type endopeptidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cellular response to stress Source: UniProtKB-HAMAP
  2. misfolded or incompletely synthesized protein catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Protein family/group databases

MEROPSiS16.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Lon proteaseUniRule annotation (EC:3.4.21.53UniRule annotation)
Alternative name(s):
ATP-dependent protease LaUniRule annotation
Gene namesi
Name:lonUniRule annotation
Ordered Locus Names:BR1106, BS1330_I1102
OrganismiBrucella suis biovar 1 (strain 1330)
Taxonomic identifieri204722 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000824: Chromosome I, UP000007104: Chromosome I

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 812812Lon proteasePRO_0000076124Add
BLAST

Proteomic databases

PRIDEiQ8G0I7.

Expressioni

Inductioni

By heat shock.UniRule annotation

Interactioni

Subunit structurei

Homohexamer. Organized in a ring with a central cavity.UniRule annotation

Protein-protein interaction databases

STRINGi204722.BR1106.

Structurei

3D structure databases

ProteinModelPortaliQ8G0I7.
SMRiQ8G0I7. Positions 502-596, 604-787.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 212189LonUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S16 family.UniRule annotation
Contains 1 Lon domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0466.
HOGENOMiHOG000261410.
KOiK01338.
OMAiIPVARMD.
OrthoDBiEOG6XHC23.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01973. lon_bact.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR027543. Lon_bac.
IPR004815. Lon_bac/euk-typ.
IPR027065. Lon_Prtase.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR003111. Pept_S16_N.
IPR008268. Peptidase_S16_AS.
IPR015947. PUA-like_domain.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10046. PTHR10046. 1 hit.
PfamiPF00004. AAA. 1 hit.
PF02190. LON. 1 hit.
PF05362. Lon_C. 1 hit.
[Graphical view]
PIRSFiPIRSF001174. Lon_proteas. 1 hit.
SMARTiSM00382. AAA. 1 hit.
SM00464. LON. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00763. lon. 1 hit.
PROSITEiPS01046. LON_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8G0I7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTGIEQKTPV GGSETGGADG LYAVLPLRDI VVFPHMIVPL FVGREKSIRA
60 70 80 90 100
LEEVMGVDKQ ILLATQKNAA DDDPAPDAIY EIGTIANVLQ LLKLPDGTVK
110 120 130 140 150
VLVEGTARAK ISKFTDREDY HEAYAAALQE PEEDAVEIEA LARSVVSDFE
160 170 180 190 200
NYVKLNKKIS PEVVGAASQI DDYSKLADTV ASHLAIKIPE KQEMLSVLSV
210 220 230 240 250
RERLEKALSF MEAEISVLQV EKRIRSRVKR QMEKTQREYY LNEQMKAIQK
260 270 280 290 300
ELGDSEDGRD EVAEIEERIT KTKLSKEARE KALAELKKLR SMSPMSAEAT
310 320 330 340 350
VVRNYLDWLL SIPWGKKSKV KQDLNFAQEV LDAEHFGLGK VKERIVGYLA
360 370 380 390 400
VQARSTKIKG PILCLVGPPG VGKTSLARSI AKATGREYVR MSLGGVRDEA
410 420 430 440 450
EIRGHRRTYI GSMPGKVIQS MKKAKKSNPL FLLDEIDKMG QDFRGDPSSA
460 470 480 490 500
MLEVLDPEQN ATFMDHYLEV EYDLSNVMFV TTANTMNIPG PLLDRMEIIR
510 520 530 540 550
IAGYTEDEKL EIAKRHLLPK AIKDHALQPK EFSVTEDALR NVIRHYTREA
560 570 580 590 600
GVRSLEREVM TLARKAVTEI LKTKKKSVKI TDKNLSDYLG VEKFRFGQID
610 620 630 640 650
GEDQVGVVTG LAWTEVGGEL LTIEGVMMPG KGRMTVTGNL RDVMKESISA
660 670 680 690 700
AASYVRSRAI DFGIEPPLFD KRDIHVHVPE GATPKDGPSA GIAMVTAIVS
710 720 730 740 750
VLTGIPVRKD IAMTGEVTLR GRVLPIGGLK EKLLAALRGG IKKVLIPEEN
760 770 780 790 800
AKDLAEIPDN VKNNLEIVPV SRVGEVLKHT LVRQPEPIEW TEQENPTAVP
810
PVEDEAGASL AH
Length:812
Mass (Da):89,795
Last modified:March 1, 2003 - v1
Checksum:i0F0AC318C9227FBF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN30026.1.
CP002997 Genomic DNA. Translation: AEM18444.1.
RefSeqiNP_698111.1. NC_004310.3.
YP_005615936.1. NC_017251.1.

Genome annotation databases

EnsemblBacteriaiAAN30026; AAN30026; BR1106.
AEM18444; AEM18444; BS1330_I1102.
GeneIDi1166782.
12138135.
KEGGibms:BR1106.
bsi:BS1330_I1102.
PATRICi17790546. VBIBruSui107850_1127.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN30026.1.
CP002997 Genomic DNA. Translation: AEM18444.1.
RefSeqiNP_698111.1. NC_004310.3.
YP_005615936.1. NC_017251.1.

3D structure databases

ProteinModelPortaliQ8G0I7.
SMRiQ8G0I7. Positions 502-596, 604-787.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi204722.BR1106.

Protein family/group databases

MEROPSiS16.001.

Proteomic databases

PRIDEiQ8G0I7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN30026; AAN30026; BR1106.
AEM18444; AEM18444; BS1330_I1102.
GeneIDi1166782.
12138135.
KEGGibms:BR1106.
bsi:BS1330_I1102.
PATRICi17790546. VBIBruSui107850_1127.

Phylogenomic databases

eggNOGiCOG0466.
HOGENOMiHOG000261410.
KOiK01338.
OMAiIPVARMD.
OrthoDBiEOG6XHC23.

Miscellaneous databases

PROiQ8G0I7.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01973. lon_bact.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR027543. Lon_bac.
IPR004815. Lon_bac/euk-typ.
IPR027065. Lon_Prtase.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR003111. Pept_S16_N.
IPR008268. Peptidase_S16_AS.
IPR015947. PUA-like_domain.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10046. PTHR10046. 1 hit.
PfamiPF00004. AAA. 1 hit.
PF02190. LON. 1 hit.
PF05362. Lon_C. 1 hit.
[Graphical view]
PIRSFiPIRSF001174. Lon_proteas. 1 hit.
SMARTiSM00382. AAA. 1 hit.
SM00464. LON. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00763. lon. 1 hit.
PROSITEiPS01046. LON_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1330.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1330.

Entry informationi

Entry nameiLON_BRUSU
AccessioniPrimary (citable) accession number: Q8G0I7
Secondary accession number(s): G0KA28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: March 1, 2003
Last modified: January 7, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella suis
    Brucella suis (strain 1330): entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.