Reviewed, UniProtKB/Swiss-Prot Q8G0H4 (ISPDF_BRUSU)

Names and origin

Protein names

Recommended name:
    Bifunctional enzyme ispD/ispF
Including the following 2 domains:
    1- Recommended name:
            2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
              EC=2.7.7.60
        Alternative name(s):
            4-diphosphocytidyl-2C-methyl-D-erythritol synthase
            MEP cytidylyltransferase
              Short name=MCT
    2- Recommended name:
            2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
                Short name=MECPS
                Short name=MECDP-synthase
              EC=4.6.1.12

Gene names

Name:	ispDF
Ordered Locus Names:	BR1120

Organism

Brucella suis [Complete proteome] [HAMAP]

Taxonomic identifier

29461 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (ispD), and converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP (ispF) By similarity. HAMAP MF_01520
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_01520 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP. HAMAP MF_01520
Cofactor	Divalent metal cations By similarity. HAMAP MF_01520
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_01520 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.
Sequence similarities	In the N-terminal section; belongs to the ispD family. In the C-terminal section; belongs to the ispF family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding
Molecular function	Lyase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity Inferred from electronic annotation. Source: HAMAP 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 390	390	Bifunctional enzyme ispD/ispF HAMAP MF_01520		PRO_0000075661
Regions
	Region	1 – 229	229	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_01520
	Region	230 – 390	161	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase HAMAP MF_01520
Sites
	Metal binding	236	1	Divalent metal cation By similarity
	Metal binding	238	1	Divalent metal cation By similarity
	Metal binding	270	1	Divalent metal cation By similarity
	Site	8	1	Transition state stabilizer By similarity
	Site	17	1	Transition state stabilizer By similarity
	Site	148	1	Positions MEP for the nucleophilic attack By similarity
	Site	205	1	Positions MEP for the nucleophilic attack By similarity
	Site	262	1	Transition state stabilizer By similarity
	Site	361	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8G0H4-1 [UniParc]. Last modified November 7, 2003. Version 2. Checksum: 06F648D2956CB10B	FASTA	390	41,840
	10 20 30 40 50 60 MAAGRGERAG QSAEGPKQYR LIGAEAVLAR TLRAFTDCPL IGTIAVVIHP DDHALYRRAV 70 80 90 100 110 120 PEKHENVILV TGGPTRQEST RLGLLALKDE APQYVLIHDG VRPFIGQDLL ERIIANLTPD 130 140 150 160 170 180 NGVLPALAVP DTLKRAAADG MVETTISRTG LFAAQTPQAF PYAPILDAHE KAFAINRTDF 190 200 210 220 230 240 TDDAAIAEWQ EIAVRIIEGS ADNTKLTWAK DIEMADKRLR QDHAVFPDIR TGNGYDVHSF 250 260 270 280 290 300 EPGDHVTLCG VKIPHEAKLN GHSDADVALH ALTDALLATR GAGDIGTHFP PSDSQWKGAA 310 320 330 340 350 360 SRIFIEHAAK IVREAGGRIA NVDVTLISEA PKIGPHRAAM TQALCDMLGI AADRVSIKAT 370 380 390 TNEKLGFVGR REGIAAIATA TVIYPGEVPE

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330 / Biovar 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN30040.1. Different initiation.
RefSeq	NP_698125.2.
3D structure databases
SMR	Q8G0H4. Positions 1-385.
ModBase	Search...
Genome annotation databases
GeneID	1166796.
GenomeReviews	Gene locus BR1120 in contig AE014291_GR.
KEGG	bms:BR1120.
NMPDR	fig|204722.1.peg.1082.
TIGR	BR1120.
Phylogenomic databases
HOGENOM	HBG672839.
PhylomeDB	Q8G0H4.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_1120-MONOMER.
BRENDA	2.7.7.60. 281610. 4.6.1.12. 281610.
Family and domain databases
HAMAP	MF_01520. IspDF. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. IPR003526. MECDP_synthase_core. IPR020555. MECDP_synthase_CS. [Graphical view]
Gene3D	G3DSA:3.30.1330.50. MECDP_synthase_core. 1 hit.
Pfam	PF01128. IspD. 1 hit. PF02542. YgbB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. ispD. 1 hit. TIGR00151. ispF. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. PS01350. ISPF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPDF_BRUSU

Accession

Primary (citable) accession number: Q8G0H4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 7, 2003
Last sequence update:	November 7, 2003
Last modified:	February 9, 2010
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)