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Protein

CTP synthase

Gene

pyrG

Organism
Brucella suis biovar 1 (strain 1330)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.UniRule annotation

Catalytic activityi

ATP + UTP + L-glutamine = ADP + phosphate + CTP + L-glutamate.UniRule annotation

Enzyme regulationi

Allosterically activated by GTP, when glutamine is the substrate. Inhibited by CTP.UniRule annotation

Pathway:iCTP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes CTP from UDP.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. CTP synthase (pyrG)
This subpathway is part of the pathway CTP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CTP from UDP, the pathway CTP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei380 – 3801NucleophileUniRule annotation
Active sitei514 – 5141UniRule annotation
Active sitei516 – 5161UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00159; UER00277.

Protein family/group databases

MEROPSiC26.964.

Names & Taxonomyi

Protein namesi
Recommended name:
CTP synthaseUniRule annotation (EC:6.3.4.2UniRule annotation)
Alternative name(s):
CTP synthetaseUniRule annotation
UTP--ammonia ligaseUniRule annotation
Gene namesi
Name:pyrGUniRule annotation
Ordered Locus Names:BR1134, BS1330_I1130
OrganismiBrucella suis biovar 1 (strain 1330)
Taxonomic identifieri204722 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000007104 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 542542CTP synthasePRO_0000138168Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ8G0G1.
SMRiQ8G0G1. Positions 2-540.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini291 – 541251Glutamine amidotransferase type-1UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 252252Aminator domainAdd
BLAST

Sequence similaritiesi

Belongs to the CTP synthase family.UniRule annotation
Contains 1 glutamine amidotransferase type-1 domain.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0504.
HOGENOMiHOG000077515.
KOiK01937.
OMAiYERFLNR.
OrthoDBiEOG6RC3NR.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_01227. PyrG.
InterProiIPR029062. Class_I_gatase-like.
IPR004468. CTP_synthase.
IPR017456. CTP_synthase_N.
IPR017926. GATASE.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11550. PTHR11550. 1 hit.
PfamiPF06418. CTP_synth_N. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00337. PyrG. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8G0G1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARYVFITGG VVSSLGKGIA AAALAALLQA RGYRVRIRKL DPYLNVDPGT
60 70 80 90 100
ISPYQHGEVF VTDDGAETDL DLGHYERFTG RPANQQDNIT TGRIYRNIIE
110 120 130 140 150
KERRGDYLGA TVQVIPHVTD EIKNFVLEGN EDYDFVLCEI GGTVGDIEAM
160 170 180 190 200
PFLEAIRQLG NELPRGTAVY IHLTLMPYIP AAGELKTKPT QHSVKELRSI
210 220 230 240 250
GIAPDILLVR ADREIPESER RKLSLFCNVR ESAVIQALDV ATIYDVPIAY
260 270 280 290 300
HKEGLDSEVL SAFGIDPAPK PRMDRWEEVS HRLHNPEGEV TIAVVGKYTG
310 320 330 340 350
LKDAYKSLIE ALHHGGLANK VKVNLDWIEA QVFESEDPAP YLEKVHGILV
360 370 380 390 400
PGGFGERGAE GKILAAKFAR ERKVPYFGIC FGMQMACIEA ARNLVGIEDA
410 420 430 440 450
SSSEFGPTRE PVVGLMTEWL KGNMLEKRAA AGDLGGTMRL GAYEAVLKPD
460 470 480 490 500
SKIAQIYGST DIHERHRHRY EVNIDYKDRL EAAGLNFAGM SPDGVLPETV
510 520 530 540
EYADHPWFIG VQYHPELKSR PFEPHPLFAS FIEAAIEQSR LV
Length:542
Mass (Da):60,058
Last modified:March 1, 2003 - v1
Checksum:iB88F10406370B7B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN30054.1.
CP002997 Genomic DNA. Translation: AEM18472.1.
RefSeqiWP_004690886.1. NZ_KN046804.1.

Genome annotation databases

EnsemblBacteriaiAAN30054; AAN30054; BR1134.
AEM18472; AEM18472; BS1330_I1130.
KEGGibms:BR1134.
bsi:BS1330_I1130.
PATRICi17790601. VBIBruSui107850_1154.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN30054.1.
CP002997 Genomic DNA. Translation: AEM18472.1.
RefSeqiWP_004690886.1. NZ_KN046804.1.

3D structure databases

ProteinModelPortaliQ8G0G1.
SMRiQ8G0G1. Positions 2-540.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC26.964.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN30054; AAN30054; BR1134.
AEM18472; AEM18472; BS1330_I1130.
KEGGibms:BR1134.
bsi:BS1330_I1130.
PATRICi17790601. VBIBruSui107850_1154.

Phylogenomic databases

eggNOGiCOG0504.
HOGENOMiHOG000077515.
KOiK01937.
OMAiYERFLNR.
OrthoDBiEOG6RC3NR.

Enzyme and pathway databases

UniPathwayiUPA00159; UER00277.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_01227. PyrG.
InterProiIPR029062. Class_I_gatase-like.
IPR004468. CTP_synthase.
IPR017456. CTP_synthase_N.
IPR017926. GATASE.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11550. PTHR11550. 1 hit.
PfamiPF06418. CTP_synth_N. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00337. PyrG. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1330.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1330.

Entry informationi

Entry nameiPYRG_BRUSU
AccessioniPrimary (citable) accession number: Q8G0G1
Secondary accession number(s): G0KA56
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: March 1, 2003
Last modified: July 22, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella suis
    Brucella suis (strain 1330): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.