Reviewed, UniProtKB/Swiss-Prot Q8G0F7 (TPIS1_BRUSU)

Names and origin

Protein names

Recommended name:
    Triosephosphate isomerase 1
      Short name=TIM 1
    EC=5.3.1.1
Alternative name(s):
    Triose-phosphate isomerase 1

Gene names

Name:	tpiA1
Synonyms:	tpiA-1
Ordered Locus Names:	BR1138

Organism

Brucella suis [Complete proteome] [HAMAP]

Taxonomic identifier

29461 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147 Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP glycolysis Inferred from electronic annotation. Source: HAMAP pentose-phosphate shunt Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	Triosephosphate isomerase 1 HAMAP MF_00147		PRO_0000090193
Sites
	Active site	99	1	Electrophile By similarity
	Active site	169	1	Proton acceptor By similarity
	Binding site	12	1	Substrate By similarity
	Binding site	14	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8G0F7-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 5E4A4A575FB4E65D	FASTA	254	26,485
	10 20 30 40 50 60 MTPGIRPLVA GNWKMNGKGE SLTELRAIAA GLSSDLGRKL DAVICVPATL LSRAAETLEG 70 80 90 100 110 120 ETVGLGGQDA HFKTSGAHTG DISPEMLKEA GATHVILGHS ERRTDHHESN KLICAKTEAA 130 140 150 160 170 180 WAAGLVAIVC VGETASERKA ERALDVIGDQ LSGSLPDGVT AENTIIAYEP VWAIGTGLTP 190 200 210 220 230 240 TVQDVRAAHA FMREQLIERF GAKGAHLRLL YGGSVKPSNA AELLGVADVD GALVGGASLK 250 AADFLAICET YRNL

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014291 Genomic DNA. Translation: AAN30058.1.
RefSeq	NP_698143.1.
3D structure databases
HSSP	HSSP built from PDB template 1NEY based on UniProtKB P00942.
ModBase	Search...
Genome annotation databases
GeneID	1166814.
GenomeReviews	Gene locus BR1138 in contig AE014291_GR.
KEGG	bms:BR1138.
TIGR	BR1138.
Phylogenomic databases
HOGENOM	Q8G0F7.
OMA	IGAQDCH.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_1138-MON.
BRENDA	5.3.1.1. 281610.
Family and domain databases
HAMAP	MF_00147. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR000652. Triosephosphate_isomerase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
ProDom	PD001005. Triophos_ismrse. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00419. tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS1_BRUSU

Accession

Primary (citable) accession number: Q8G0F7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)