Reviewed,
UniProtKB/Swiss-Prot Q8G0F1 (LEXA_BRUSU)
Last modified
February 9, 2010.
Version 49.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: LexA repressor EC=3.4.21.88 | ||||
| Gene names |
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| Organism | Brucella suis [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 29461 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella |
Protein attributes
| Sequence length | 240 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity. HAMAP MF_00015 |
| Catalytic activity | Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP MF_00015 |
| Subunit structure | Homodimer By similarity. HAMAP MF_00015 |
| Sequence similarities | Belongs to the peptidase S24 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation |
| Ligand | DNA-binding |
| Molecular function | Hydrolase Repressor |
| PTM | Autocatalytic cleavage |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | DNA repair Inferred from electronic annotation. Source: HAMAP DNA replicationInferred from electronic annotation. Source: HAMAP SOS responseInferred from electronic annotation. Source: HAMAP negative regulation of transcription, DNA-dependentInferred from electronic annotation. Source: HAMAP proteolysisInferred from electronic annotation. Source: InterPro transcriptionInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | DNA binding Inferred from electronic annotation. Source: HAMAP serine-type endopeptidase activityInferred from electronic annotation. Source: HAMAP |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 240 | 240 | LexA repressor HAMAP MF_00015 | PRO_0000170020 | |||||
Regions | |||||||||
| DNA binding | 26 – 46 | 21 | H-T-H motif By similarity | ||||||
Sites | |||||||||
| Active site | 161 | 1 | For autocatalytic cleavage activity By similarity | ||||||
| Active site | 199 | 1 | For autocatalytic cleavage activity By similarity | ||||||
| Site | 126 – 127 | 2 | Cleavage; by autolysis By similarity | ||||||
Sequences
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References
| [1] | "The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. Fraser C.M.Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330 / Biovar 1. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AE014291 Genomic DNA. Translation: AAN30064.1. |
| RefSeq | NP_698149.1. |
3D structure databases | |
| SMR | Q8G0F1. Positions 117-240. |
| ModBase | Search... |
Protein family/group databases | |
| MEROPS | S24.001. |
Genome annotation databases | |
| GeneID | 1166820. |
| GenomeReviews | Gene locus BR1144 in contig AE014291_GR. |
| KEGG | bms:BR1144. |
| TIGR | BR1144. |
Phylogenomic databases | |
| HOGENOM | HBG679610. |
| OMA | AILTFIR. |
| PhylomeDB | Q8G0F1. |
Enzyme and pathway databases | |
| BioCyc | BSUI204722:BR_1144-MONOMER. |
| BRENDA | 3.4.21.88. 281610. |
Family and domain databases | |
| HAMAP | MF_00015. LexA. [Tree] |
| InterPro | IPR006199. LexA_DNA-bd_dom. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA_cons-reg. IPR019759. Peptidase_S24_S26_cons-reg. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view] |
| Gene3D | G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit. |
| Pfam | PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view] |
| PRINTS | PR00726. LEXASERPTASE. |
| TIGRFAMs | TIGR00498. lexA. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | LEXA_BRUSU | ||||||||
| Accession | Primary (citable) accession number: Q8G0F1 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Brucella suis Brucella suis (strain 1330): entries and gene names |
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

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