Q8G0F1 (LEXA_BRUSU) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 62.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: LexA repressor EC=3.4.21.88 | ||||
| Gene names |
| ||||
| Organism | Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 204722 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella |
Protein attributes
| Sequence length | 240 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity. HAMAP MF_00015 |
| Catalytic activity | Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP MF_00015 |
| Subunit structure | Homodimer By similarity. HAMAP MF_00015 |
| Sequence similarities | Belongs to the peptidase S24 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation |
| Ligand | DNA-binding |
| Molecular function | Hydrolase Repressor |
| PTM | Autocatalytic cleavage |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replicationInferred from electronic annotation. Source: UniProtKB-KW SOS responseInferred from electronic annotation. Source: UniProtKB-KW proteolysisInferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependentInferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependentInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | DNA binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 240 | 240 | LexA repressor HAMAP MF_00015 | PRO_0000170020 | |||||
Regions | |||||||||
| DNA binding | 26 – 46 | 21 | H-T-H motif By similarity | ||||||
Sites | |||||||||
| Active site | 161 | 1 | For autocatalytic cleavage activity By similarity | ||||||
| Active site | 199 | 1 | For autocatalytic cleavage activity By similarity | ||||||
| Site | 126 – 127 | 2 | Cleavage; by autolysis By similarity | ||||||
Sequences
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References
| [1] | "The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. Fraser C.M.Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330. |
| [2] | "Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed: 22038969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AE014291 Genomic DNA. Translation: AAN30064.1. CP002997 Genomic DNA. Translation: AEM18482.1. |
| RefSeq | NP_698149.1. NC_004310.3. |
3D structure databases | |
| ProteinModelPortal | Q8G0F1. |
| ModBase | Search... |
Protein family/group databases | |
| MEROPS | S24.001. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 1166820. |
| GenomeReviews | Gene locus BR1144 in contig AE014291_GR. |
| KEGG | bms:BR1144. |
| PATRIC | 17790621. VBIBruSui107850_1164. |
| TIGR | BR1144. |
Phylogenomic databases | |
| HOGENOM | HBG679610. |
| OMA | AILTFIR. |
| PhylomeDB | Q8G0F1. |
| ProtClustDB | PRK00215. |
Enzyme and pathway databases | |
| BioCyc | BSUI204722:BR_1144-MONOMER. |
Family and domain databases | |
| HAMAP | MF_00015. LexA. [Tree] |
| InterPro | IPR006199. LexA_DNA-bd_dom. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA. IPR019759. Peptidase_S24_S26. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view] |
| Gene3D | G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit. |
| KO | K01356. |
| Pfam | PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view] |
| PRINTS | PR00726. LEXASERPTASE. |
| SUPFAM | SSF51306. Pept_S24_S26_C. 1 hit. |
| TIGRFAMs | TIGR00498. LexA. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | LEXA_BRUSU | ||||||||
| Accession | Primary (citable) accession number: Q8G0F1 Secondary accession number(s): G0KA66 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| Brucella suis Brucella suis (strain 1330): entries and gene names |
| SIMILARITY comments Index of protein domains and families |

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