Q8G085 (RL5_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
50S ribosomal protein L5

Gene names

Name:	rplE
Ordered Locus Names:	BR1221, BS1330_I1217

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella suis

Protein attributes

Sequence length	185 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. Contacts the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs By similarity. HAMAP-Rule MF_01333
Subunit structure	Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S rRNA and the P site tRNA. Forms a bridge to the 30S subunit in the 70S ribosome By similarity.
Sequence similarities	Belongs to the ribosomal protein L5P family.

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding tRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Cellular_component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 185	185	50S ribosomal protein L5 HAMAP-Rule MF_01333		PRO_0000124902

Sequences

	Sequence		Length	Mass (Da)
	Q8G085 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 22059A13FB6BDF5A	FASTA	185	21,074
	10 20 30 40 50 60 MAEAKALPRF KKLYQDNIRK ALLEEFKYDN EMQIPRITKV VLNMGVGEAT GDSKKPAVAA 70 80 90 100 110 120 EDLAMIAGQK AVVTRARNSI ATFKLREGMP IGAKVTLRQD RMYEFLDRLI TIALPRVRDF 130 140 150 160 170 180 RGLNPKSFDG RGNYAMGIKE HIVFPEINYD KVDQIWGMDI IVCTTAKTDD EARSLLRAFN FPFRQ

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN30140.1. CP002997 Genomic DNA. Translation: AEM18558.1.
PIR	AC3348.
RefSeq	NP_698225.1. NC_004310.3. YP_005616050.1. NC_017251.1.
3D structure databases
ProteinModelPortal	Q8G085.
SMR	Q8G085. Positions 8-185.
ModBase	Search...
Protein-protein interaction databases
STRING	204722.BR1221.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN30140; AAN30140; BR1221. AEM18558; AEM18558; BS1330_I1217.
GeneID	1166897. 12138250.
KEGG	bms:BR1221. bsi:BS1330_I1217.
PATRIC	17790779. VBIBruSui107850_1243.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0094.
HOGENOM	HOG000231311.
KO	K02931.
OMA	EQVMFHE.
ProtClustDB	PRK00010.
Family and domain databases
Gene3D	3.30.1440.10. 1 hit.
HAMAP	MF_01333_B. Ribosomal_L5_B.
InterPro	IPR002132. Ribosomal_L5. IPR020930. Ribosomal_L5_bac-type. IPR020929. Ribosomal_L5_CS. IPR022803. Ribosomal_L5_domain. [Graphical view]
PANTHER	PTHR11994. PTHR11994. 1 hit.
Pfam	PF00281. Ribosomal_L5. 1 hit. PF00673. Ribosomal_L5_C. 1 hit. [Graphical view]
PIRSF	PIRSF002161. Ribosomal_L5. 1 hit.
SUPFAM	SSF55282. Ribosomal_L5. 1 hit.
PROSITE	PS00358. RIBOSOMAL_L5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RL5_BRUSU

Accession

Primary (citable) accession number: Q8G085
Secondary accession number(s): G0KAE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program