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Q8G075 (EFG_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Elongation factor G

Short name=EF-G
Gene names
Name:fusA
Ordered Locus Names:BR1236, BS1330_I1232
OrganismBrucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]
Taxonomic identifier204722 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length694 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome By similarity. HAMAP-Rule MF_00054

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00054.

Sequence similarities

Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandGTP-binding
Nucleotide-binding
   Molecular functionElongation factor
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

GTPase activity

Inferred from electronic annotation. Source: InterPro

translation elongation factor activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 694694Elongation factor G HAMAP-Rule MF_00054
PRO_0000091089

Regions

Nucleotide binding17 – 248GTP By similarity
Nucleotide binding86 – 905GTP By similarity
Nucleotide binding140 – 1434GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8G075 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 1EC9ABC88B769A59

FASTA69476,265
        10         20         30         40         50         60 
MAREYKIEDY RNFGIMAHID AGKTTMTERI LFYTGKNHKI GETHDGASTM DWMEQEQERG 

        70         80         90        100        110        120 
ITITSAATTT FWQGRDGKKR RFNIIDTPGH VDFTIEVERS LRVLDGAIAL LDANAGVEPQ 

       130        140        150        160        170        180 
TETVWRQAEK YHVPRMVFVN KMDKIGADFY RSVEMVGSRL GAVALPVQLP IGAENDFVGV 

       190        200        210        220        230        240 
VDLIEMKALT WDGTIGAPAT VGEIPADMAD KAEEYREKLI ELAVEIDEAA MEAYLEGTMP 

       250        260        270        280        290        300 
TNDELRALIR KGTIEVKFHP ILCGTAFKNR GVQPLLDAVV EFLPAPTDVP AIKGIDVKTE 

       310        320        330        340        350        360 
TETTRESSDE APLSMLAFKI MNDPFVGSLT FARIYSGKLT KGVSLENTVK GKRERIGRML 

       370        380        390        400        410        420 
QMHSNSREDI DEAFAGDIVA LAGLKETTTG DTLCDPLKPV ILERMEFPDP VIEIAIEPKT 

       430        440        450        460        470        480 
KADQEKMGIA LNRLAAEDPS FRVKSDEESG QTIIAGMGEL HLDILVDRMK REFKVEANVG 

       490        500        510        520        530        540 
APQVAYRESI TRAAEIDYTH KKQSGGSGQF ARVKIIFEPH DGDDFIFESK IVGGSVPKEY 

       550        560        570        580        590        600 
IPGVQKGIES VMGAGPLAGF PMLGVKATLI DGAYHDVDSS VLAFEIASRA AFREGAQKAG 

       610        620        630        640        650        660 
AQLLEPIMKV EVVTPEDYVG DVIGDLNSRR GQISGTEARG IATVVNAMVP LANMFGYVNS 

       670        680        690 
LRSMSQGRAQ YTMQFDHYEP VPTAVAQEIQ KKFA 

« Hide

References

[1]"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts."
Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. expand/collapse author list , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.
[2]"Revised genome sequence of Brucella suis 1330."
Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.
J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014291 Genomic DNA. Translation: AAN30155.1.
CP002997 Genomic DNA. Translation: AEM18573.1.
RefSeqNP_698240.1. NC_004310.3.
YP_005616065.1. NC_017251.1.

3D structure databases

ProteinModelPortalQ8G075.
SMRQ8G075. Positions 4-691.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING204722.BR1236.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN30155; AAN30155; BR1236.
AEM18573; AEM18573; BS1330_I1232.
GeneID1166912.
12138265.
KEGGbms:BR1236.
bsi:BS1330_I1232.
PATRIC17790809. VBIBruSui107850_1258.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0480.
HOGENOMHOG000231585.
KOK02355.
OMASGVQPQT.
OrthoDBEOG6X6RBF.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00054_B. EF_G_EF_2_B.
InterProIPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004540. Transl_elong_EFG/EF2.
IPR005517. Transl_elong_EFG/EF2_IV.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSPR00315. ELONGATNFCT.
SMARTSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsTIGR00484. EF-G. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameEFG_BRUSU
AccessionPrimary (citable) accession number: Q8G075
Secondary accession number(s): G0KAF7
Entry history
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Brucella suis

Brucella suis (strain 1330): entries and gene names