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Q8G069 (RPOB_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase subunit beta

Short name=RNAP subunit beta
EC=2.7.7.6
Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta
Gene names
Name:rpoB
Ordered Locus Names:BR1243, BS1330_I1239
OrganismBrucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]
Taxonomic identifier204722 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length1377 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates By similarity. HAMAP-Rule MF_01321

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP-Rule MF_01321

Subunit structure

The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.

Sequence similarities

Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
   Biological processTranscription
   Cellular componentDNA-directed RNA polymerase
   Molecular functionNucleotidyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtranscription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

DNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

ribonucleoside binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13771377DNA-directed RNA polymerase subunit beta HAMAP-Rule MF_01321
PRO_0000047871

Sequences

Sequence LengthMass (Da)Tools
Q8G069 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 5523F3E2A260F57F

FASTA1,377153,641
        10         20         30         40         50         60 
MAQTHSFNGR KRVRKFFGKI PEVAEMPNLI EVQKASYDQF LMVEEPSGGR PDEGLQAVFK 

        70         80         90        100        110        120 
SVFPIQDFSG ASMLEFVRYE FDPPKFDVDE CRQRDLTYSA PLKVTLRLIV FDIDEDTGAK 

       130        140        150        160        170        180 
SIKDIKEQDV YMGDMPLMTD NGTFIVNGTE RVIVSQMHRS PGVFFDHDKG KTHSSGKLLF 

       190        200        210        220        230        240 
AARVIPYRGS WLDIEFDSKD IVYARIDRRR KLPATTLLMA LGMDGEEILS TFYKTVTYTR 

       250        260        270        280        290        300 
DGDNWRIPYS AERFKGMKII SDLVDADTGE AVLEAGKKLT ARAAKQLAEK GLKAIKATED 

       310        320        330        340        350        360 
DLFGSYLAED VVNYATGEIY LEAGDEIDEK VLKTLIDTGE TEINVLDIDH VNIGAYIRNT 

       370        380        390        400        410        420 
LAVDKNESRQ EALFDIYRVM RPGEPPTMDS AEAMFHSLFF DSERYDLSAV GRVKMNMRLD 

       430        440        450        460        470        480 
LDAEDTVRVL RKEDILAVVK MLVELRDGRG EIDDIDNLGN RRVRSVGELM ENQYRVGLLR 

       490        500        510        520        530        540 
MERAIKERMS SIEIDTVMPQ DLINAKPAAA AVREFFGSSQ LSQFMDQTNP LSEITHKRRL 

       550        560        570        580        590        600 
SALGPGGLTR ERAGFEVRDV HPTHYGRICP IETPEGPNIG LINSLATFAR VNKYGFIESP 

       610        620        630        640        650        660 
YRKVVDGKVT NDVVYLSAME EAKHSVAQAN VELDEQGGFV DEFVICRHAG EVMMAPRENV 

       670        680        690        700        710        720 
DLMDVSPKQL VSVAAALIPF LENDDANRAL MGSNMQRQAV PLVRAEAPFV GTGMEPIVAR 

       730        740        750        760        770        780 
DSGAAIAARR GGIVDQVDAT RIVIRATEEL DPSKSGVDIY RLQKFQRSNQ STCINQRPLV 

       790        800        810        820        830        840 
RVGDRIHKGD IIADGPSTDL GDLALGRNVL VAFMPWNGYN YEDSILLSEK IVSDDVFTSI 

       850        860        870        880        890        900 
HIEEFEVAAR DTKLGPEEIT RDIPNVSEEA LKNLDEAGIV YIGAEVHPGD ILVGKITPKG 

       910        920        930        940        950        960 
ESPMTPEEKL LRAIFGEKAS DVRDTSMRMP PGTYGTVVEV RVFNRHGVEK DERAMAIERE 

       970        980        990       1000       1010       1020 
EIERLAKDRD DEQAILDRNV YGRLADMIDG KVAAAGPKGF KKGTTITREL MTEYPRSQWW 

      1030       1040       1050       1060       1070       1080 
QFAVEDEKLQ GELEALRSQY DDSKKLLEAR FMDKVEKVQR GDEMPPGVMK MVKVFVAVKR 

      1090       1100       1110       1120       1130       1140 
KIQPGDKMAG RHGNKGVVSR ILPVEDMPFL EDGTHADIVL NPLGVPSRMN VGQILETHLG 

      1150       1160       1170       1180       1190       1200 
WACAGMGKKI GELLDVYRKT ANIEPLRQTL EHIYPDNDRN EPVRSYDDDA ILMLANQVKR 

      1210       1220       1230       1240       1250       1260 
GVSIATPVFD GAVEADINAM LTDAGLATSG QSTLYDGRTG EPFDRQVTMG YIYMLKLHHL 

      1270       1280       1290       1300       1310       1320 
VDDKIHARSI GPYSLVTQQP LGGKAQFGGQ RFGEMEVWAL EAYGAAYTLQ EMLTVKSDDV 

      1330       1340       1350       1360       1370 
AGRTKVYEAI VRGDDTFEAG IPESFNVLVK EMRSLGLNVE LDDTREAEQP ALPDAAE 

« Hide

References

[1]"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts."
Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. expand/collapse author list , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.
[2]"Revised genome sequence of Brucella suis 1330."
Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.
J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014291 Genomic DNA. Translation: AAN30162.1.
CP002997 Genomic DNA. Translation: AEM18580.1.
RefSeqNP_698247.1. NC_004310.3.
YP_005616072.1. NC_017251.1.

3D structure databases

ProteinModelPortalQ8G069.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING204722.BR1243.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN30162; AAN30162; BR1243.
AEM18580; AEM18580; BS1330_I1239.
GeneID1166919.
12138272.
KEGGbms:BR1243.
bsi:BS1330_I1239.
PATRIC17790823. VBIBruSui107850_1265.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0085.
HOGENOMHOG000218612.
KOK03043.
OMAFPITDFS.
OrthoDBEOG6M9DS6.

Family and domain databases

Gene3D2.30.150.10. 1 hit.
2.40.270.10. 2 hits.
2.40.50.150. 2 hits.
3.90.1110.10. 2 hits.
HAMAPMF_01321. RNApol_bact_RpoB.
InterProIPR010243. DNA-dir_RNA_pol_bsu.
IPR019462. DNA-dir_RNA_pol_bsu_external_1.
IPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
PANTHERPTHR20856. PTHR20856. 1 hit.
PfamPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 2 hits.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF10385. RNA_pol_Rpb2_45. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
TIGRFAMsTIGR02013. rpoB. 1 hit.
PROSITEPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ8G069.

Entry information

Entry nameRPOB_BRUSU
AccessionPrimary (citable) accession number: Q8G069
Secondary accession number(s): G0KAG4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: March 1, 2003
Last modified: June 11, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Brucella suis

Brucella suis (strain 1330): entries and gene names