Q8G069 (RPOB_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta
Short name=RNAP subunit beta
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta

Gene names

Name:	rpoB
Ordered Locus Names:	BR1243, BS1330_I1239

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	1377 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01321
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01321
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1377	1377	DNA-directed RNA polymerase subunit beta HAMAP MF_01321		PRO_0000047871

Sequences

	Sequence		Length	Mass (Da)
	Q8G069 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 5523F3E2A260F57F	FASTA	1,377	153,641
	10 20 30 40 50 60 MAQTHSFNGR KRVRKFFGKI PEVAEMPNLI EVQKASYDQF LMVEEPSGGR PDEGLQAVFK 70 80 90 100 110 120 SVFPIQDFSG ASMLEFVRYE FDPPKFDVDE CRQRDLTYSA PLKVTLRLIV FDIDEDTGAK 130 140 150 160 170 180 SIKDIKEQDV YMGDMPLMTD NGTFIVNGTE RVIVSQMHRS PGVFFDHDKG KTHSSGKLLF 190 200 210 220 230 240 AARVIPYRGS WLDIEFDSKD IVYARIDRRR KLPATTLLMA LGMDGEEILS TFYKTVTYTR 250 260 270 280 290 300 DGDNWRIPYS AERFKGMKII SDLVDADTGE AVLEAGKKLT ARAAKQLAEK GLKAIKATED 310 320 330 340 350 360 DLFGSYLAED VVNYATGEIY LEAGDEIDEK VLKTLIDTGE TEINVLDIDH VNIGAYIRNT 370 380 390 400 410 420 LAVDKNESRQ EALFDIYRVM RPGEPPTMDS AEAMFHSLFF DSERYDLSAV GRVKMNMRLD 430 440 450 460 470 480 LDAEDTVRVL RKEDILAVVK MLVELRDGRG EIDDIDNLGN RRVRSVGELM ENQYRVGLLR 490 500 510 520 530 540 MERAIKERMS SIEIDTVMPQ DLINAKPAAA AVREFFGSSQ LSQFMDQTNP LSEITHKRRL 550 560 570 580 590 600 SALGPGGLTR ERAGFEVRDV HPTHYGRICP IETPEGPNIG LINSLATFAR VNKYGFIESP 610 620 630 640 650 660 YRKVVDGKVT NDVVYLSAME EAKHSVAQAN VELDEQGGFV DEFVICRHAG EVMMAPRENV 670 680 690 700 710 720 DLMDVSPKQL VSVAAALIPF LENDDANRAL MGSNMQRQAV PLVRAEAPFV GTGMEPIVAR 730 740 750 760 770 780 DSGAAIAARR GGIVDQVDAT RIVIRATEEL DPSKSGVDIY RLQKFQRSNQ STCINQRPLV 790 800 810 820 830 840 RVGDRIHKGD IIADGPSTDL GDLALGRNVL VAFMPWNGYN YEDSILLSEK IVSDDVFTSI 850 860 870 880 890 900 HIEEFEVAAR DTKLGPEEIT RDIPNVSEEA LKNLDEAGIV YIGAEVHPGD ILVGKITPKG 910 920 930 940 950 960 ESPMTPEEKL LRAIFGEKAS DVRDTSMRMP PGTYGTVVEV RVFNRHGVEK DERAMAIERE 970 980 990 1000 1010 1020 EIERLAKDRD DEQAILDRNV YGRLADMIDG KVAAAGPKGF KKGTTITREL MTEYPRSQWW 1030 1040 1050 1060 1070 1080 QFAVEDEKLQ GELEALRSQY DDSKKLLEAR FMDKVEKVQR GDEMPPGVMK MVKVFVAVKR 1090 1100 1110 1120 1130 1140 KIQPGDKMAG RHGNKGVVSR ILPVEDMPFL EDGTHADIVL NPLGVPSRMN VGQILETHLG 1150 1160 1170 1180 1190 1200 WACAGMGKKI GELLDVYRKT ANIEPLRQTL EHIYPDNDRN EPVRSYDDDA ILMLANQVKR 1210 1220 1230 1240 1250 1260 GVSIATPVFD GAVEADINAM LTDAGLATSG QSTLYDGRTG EPFDRQVTMG YIYMLKLHHL 1270 1280 1290 1300 1310 1320 VDDKIHARSI GPYSLVTQQP LGGKAQFGGQ RFGEMEVWAL EAYGAAYTLQ EMLTVKSDDV 1330 1340 1350 1360 1370 AGRTKVYEAI VRGDDTFEAG IPESFNVLVK EMRSLGLNVE LDDTREAEQP ALPDAAE

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed: 22038969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN30162.1. CP002997 Genomic DNA. Translation: AEM18580.1.
RefSeq	NP_698247.1. NC_004310.3.
3D structure databases
ProteinModelPortal	Q8G069.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1166919.
GenomeReviews	Gene locus BR1243 in contig AE014291_GR.
KEGG	bms:BR1243.
NMPDR	fig|204722.1.peg.1204.
PATRIC	17790823. VBIBruSui107850_1265.
TIGR	BR1243.
Phylogenomic databases
HOGENOM	HBG285547.
OMA	QRDLTYA.
PhylomeDB	Q8G069.
ProtClustDB	PRK00405.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_1243-MONOMER.
Family and domain databases
HAMAP	MF_01321. RNApol_bact_RpoB. [Tree]
InterPro	IPR010243. DNA-dir_RNA_pol_bsu_bac-type. IPR019462. DNA-dir_RNA_pol_bsu_external_1. IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
Gene3D	G3DSA:2.30.150.10. G3DSA:2.30.150.10. 1 hit. G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits. G3DSA:2.40.50.150. Ribosomal_L2. 2 hits.
KO	K03043.
PANTHER	PTHR20856:SF3. PTHR20856:SF3. 1 hit. PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 2 hits. PF04565. RNA_pol_Rpb2_3. 1 hit. PF10385. RNA_pol_Rpb2_45. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR02013. RpoB. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB_BRUSU

Accession

Primary (citable) accession number: Q8G069
Secondary accession number(s): G0KAG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program