Q8FZV8 (URED2_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Urease accessory protein UreD 2

Gene names

Name:	ureD2
Synonyms:	ureD-2
Ordered Locus Names:	BR1362, BS1330_I1357

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella suis

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for maturation of urease via the functional incorporation of the urease nickel metallocenter By similarity. Ref.3 Disrupting the ure2 operon has no effect on urease activity, or pathogen survival in BALB/c mice when innoculated by gavage, but confers slightly enhanced resistance to low pH killing in vitro. Ref.3
Subunit structure	UreD, UreF and UreG form a complex that acts as a GTP-hydrolysis-dependent molecular chaperone, activating the urease apoprotein by helping to assemble the nickel containing metallocenter of UreC. The UreE protein probably delivers the nickel By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the UreD family.

Ontologies

Keywords
Biological process	Nickel insertion
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	nitrogen compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	nickel cation binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	Urease accessory protein UreD 2 HAMAP-Rule MF_01384		PRO_0000340428

Sequences

	Sequence		Length	Mass (Da)
	Q8FZV8 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: BD228D3B2E437E06	FASTA	302	33,685
	10 20 30 40 50 60 MLGKARELAN YQDEPEQLPT GSFGKNAFLR LGFERRPERT VLATLHRRAP LIVQQALYWD 70 80 90 100 110 120 EGMPTLPCVS IISNAGGILQ GDRYAIEIDL EPDTQAHVTT QSATRIQEMD ANFATQTQTI 130 140 150 160 170 180 TLGANSYLEY IPHPIIPHKH SRFVQQTEVT IHPTATLIYS EVLMAGRKYY GTGELFHYDL 190 200 210 220 230 240 FSSKFHAAHT DGTSLFTEKF IVEPARGNVS RLGAMGSFHV FGNLILLTPK THADRLFETI 250 260 270 280 290 300 DPVFDMDEGI AWGASRLPND AGLLFKVLGM ESAPVRAAIR KIWEAARQEV TGASLPENFL WA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.
[2]	"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.
[3]	"Brucella suis urease encoded by ure1 but not ure2 is necessary for intestinal infection of BALB/c mice." Bandara A.B., Contreras A., Contreras-Rodriguez A., Martins A.M., Dobrean V., Poff-Reichow S., Rajasekaran P., Sriranganathan N., Schurig G.G., Boyle S.M. BMC Microbiol. 7:57-57(2007) [PubMed] [Europe PMC] [Abstract] Cited for: OPERON DISRUPTION, LACK OF ROLE IN VIRULENCE. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN30276.1. CP002997 Genomic DNA. Translation: AEM18693.1.
PIR	AE3332.
RefSeq	NP_698361.1. NC_004310.3. YP_005616185.1. NC_017251.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	204722.BR1362.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN30276; AAN30276; BR1362. AEM18693; AEM18693; BS1330_I1357.
GeneID	1167044. 12138396.
KEGG	bms:BR1362. bsi:BS1330_I1357.
PATRIC	17791075. VBIBruSui107850_1384.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0829.
HOGENOM	HOG000060675.
KO	K03190.
OMA	LYWDEAL.
ProtClustDB	CLSK898180.
Family and domain databases
HAMAP	MF_01384. UreD.
InterPro	IPR002669. UreD. [Graphical view]
Pfam	PF01774. UreD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

URED2_BRUSU

Accession

Primary (citable) accession number: Q8FZV8
Secondary accession number(s): G0KB47

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program