Q8FZQ3 (DNLJ_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA ligase
EC=6.5.1.2

Alternative name(s):
Polydeoxyribonucleotide synthase [NAD+]

Gene names

Name:	ligA
Ordered Locus Names:	BR1420, BS1330_I1414

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	719 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA By similarity. HAMAP MF_01588
Catalytic activity	NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m). HAMAP MF_01588
Cofactor	Magnesium or manganese By similarity. HAMAP MF_01588
Sequence similarities	Belongs to the NAD-dependent DNA ligase family. LigA subfamily. Contains 1 BRCT domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Ligand	Magnesium Manganese Metal-binding NAD Zinc
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (NAD+) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 719	719	DNA ligase HAMAP MF_01588		PRO_0000313156
Regions
	Domain	640 – 719	80	BRCT
	Nucleotide binding	42 – 46	5	NAD By similarity
	Nucleotide binding	92 – 93	2	NAD By similarity
Sites
	Active site	128	1	N6-AMP-lysine intermediate By similarity
	Metal binding	430	1	Zinc By similarity
	Metal binding	433	1	Zinc By similarity
	Metal binding	448	1	Zinc By similarity
	Metal binding	454	1	Zinc By similarity
	Binding site	126	1	NAD By similarity
	Binding site	149	1	NAD By similarity
	Binding site	185	1	NAD By similarity
	Binding site	301	1	NAD By similarity
	Binding site	325	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FZQ3 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 122949D2FB8B1B79	FASTA	719	78,681
	10 20 30 40 50 60 MSDISVEKLT ELEAAAELER LARAIAHHDE LYHAKDRPEI SDAAYDALKR RNEAIEAHFP 70 80 90 100 110 120 ALVRDDSPSR RVGAAPALAT FAPVVHARPM LSLDNAFSDE DVRDFVGSVY RFLGQLPDDS 130 140 150 160 170 180 IAFTAEPKID GLSMSIRYEN GILVSGATRG DGTTGENVTA NIRTIAEIPN RLPAGAPAVV 190 200 210 220 230 240 EVRGEVYMAK SDFLTLNAQM EAEGKQTYVN PRNTAAGSLR QLDAKVTASR KLRFFAYAWG 250 260 270 280 290 300 EMSDMPADTQ LGMVEVFRQW GFPVNPLMKR FNSVDGLLAH YRAIGMERPT LDYDIDGVVY 310 320 330 340 350 360 KVDRLDLQTR LGFRSRSPRW AIAHKFPAEQ ALTILRGIDI QVGRTGALTP VARLEPITVG 370 380 390 400 410 420 GVVVTNATLH NEDYIKGIGQ KGEPIREGRD IRIGDSVIVQ RAGDVIPQIV DVVLEEGKKR 430 440 450 460 470 480 GEPYQFPHVC PACGSHAVRE EGEAVRRCTG GLICPAQAVE RIRHFVSRNA FDIEGLGEKQ 490 500 510 520 530 540 VEFFFNAEDP ALCIRSPADI FTLKKRQENS LTKLQNIEGF GATSVKKLYD AIDARREIAL 550 560 570 580 590 600 HRFLFGLGIR HVGEVNAKRL ARAYLSYAAF EKAALEAVPP KEGDRTDKGS EAWQDMLAVE 610 620 630 640 650 660 GIGSIVAEAV VDFYGEPHNR EVLAALLAEV TPLDEEARVA TGSPVEGKTV VFTGSLERMS 670 680 690 700 710 RDEAKAMAER HGAKTAGSVS KKTDLVVAGP GAGSKLAKAT ELGIEVINED DWFKLVGED

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed: 22038969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN30333.1. CP002997 Genomic DNA. Translation: AEM18749.1.
RefSeq	NP_698418.1. NC_004310.3.
3D structure databases
HSSP	HSSP built from PDB template 1B04 based on UniProtKB O87703.
ProteinModelPortal	Q8FZQ3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1167102.
GenomeReviews	Gene locus BR1420 in contig AE014291_GR.
KEGG	bms:BR1420.
PATRIC	17791199. VBIBruSui107850_1446.
TIGR	BR1420.
Phylogenomic databases
HOGENOM	HBG620317.
OMA	ENVRTIR.
PhylomeDB	Q8FZQ3.
ProtClustDB	PRK07956.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_1420-MONOMER.
Family and domain databases
HAMAP	MF_01588. DNA_ligase_A. [Tree]
InterPro	IPR001357. BRCT. IPR018239. DNA_ligase_AS. IPR004150. DNA_ligase_OB. IPR001679. DNAligase. IPR013839. DNAligase_adenylation. IPR013840. DNAligase_N. IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR010994. RuvA_2-like. IPR004149. Znf_DNAligase_C4. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01972.
Pfam	PF00533. BRCT. 1 hit. PF01653. DNA_ligase_aden. 1 hit. PF03120. DNA_ligase_OB. 1 hit. PF03119. DNA_ligase_ZBD. 1 hit. [Graphical view]
PIRSF	PIRSF001604. LigA. 1 hit.
SMART	SM00292. BRCT. 1 hit. SM00278. HhH1. 2 hits. SM00532. LIGANc. 1 hit. [Graphical view]
SUPFAM	SSF52113. BRCT. 1 hit. SSF50249. Nucleic_acid_OB. 1 hit. SSF47781. RuvA_2_like. 1 hit.
TIGRFAMs	TIGR00575. Dnlj. 1 hit.
PROSITE	PS50172. BRCT. 1 hit. PS01055. DNA_LIGASE_N1. 1 hit. PS01056. DNA_LIGASE_N2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLJ_BRUSU

Accession

Primary (citable) accession number: Q8FZQ3
Secondary accession number(s): G0KBI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program