Reviewed, UniProtKB/Swiss-Prot Q8FZP4 (MURB_BRUSU)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylenolpyruvoylglucosamine reductase
    EC=1.1.1.158
Alternative name(s):
    UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	BR1429

Organism

Brucella suis [Complete proteome] [HAMAP]

Taxonomic identifier

29461 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00037
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity. HAMAP MF_00037
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm Probable HAMAP MF_00037.
Sequence similarities	Belongs to the murB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000179186
Regions
	Domain	36 – 202	167	FAD-binding PCMH-type
Sites
	Active site	182	1	By similarity
	Active site	231	1	Proton donor By similarity
	Active site	301	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FZP4-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 2A18976B954176CA	FASTA	322	34,891
	10 20 30 40 50 60 MMESGEALLK KLDGRLSGLR GRLTPDTGMD KITWFRAGGP AQVLFQPSDE EDLSAFLKAV 70 80 90 100 110 120 PEEIPLLVVG IGSNLLVRDG GVPGFVVRLS AKGFGEVEQV CDTQLRAGAA APDKRVAAAA 130 140 150 160 170 180 LEAGLAGFHF YHGIPGGVGG ALRMNAGANG VETRERVVEV RALDRKGEVH VLSNADMGYA 190 200 210 220 230 240 YRHSSASPDL IFTSVLFEGV PGERDDIRRA MDEVQHHRET VQPVREKTGG STFKNPEGTS 250 260 270 280 290 300 AWKEIDKAGC RGLRVGGAQM SEMHCNFMIN TGNATGHDLE TLGETVRARV FENSGIRLHW 310 320 EIKRLGLFRE GEQIEEFLGK II

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330 / Biovar 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN30342.1.
RefSeq	NP_698427.1.
3D structure databases
SMR	Q8FZP4. Positions 26-303.
ModBase	Search...
Genome annotation databases
GeneID	1167111.
GenomeReviews	Gene locus BR1429 in contig AE014291_GR.
KEGG	bms:BR1429.
NMPDR	fig|204722.1.peg.1384.
TIGR	BR1429.
Phylogenomic databases
HOGENOM	HBG686573.
OMA	QPIREKT.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_1429-MONOMER.
BRENDA	1.1.1.158. 281610.
Family and domain databases
HAMAP	MF_00037. MurB. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00179. murB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_BRUSU

Accession

Primary (citable) accession number: Q8FZP4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	March 1, 2003
Last modified:	February 9, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)