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Q8FZP2 (MURD_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase

EC=6.3.2.9
Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
Gene names
Name:murD
Ordered Locus Names:BR1433, BS1330_I1427
OrganismBrucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]
Taxonomic identifier204722 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length467 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639

Catalytic activity

ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639

Subcellular location

Cytoplasm By similarity HAMAP MF_00639.

Sequence similarities

Belongs to the MurCDEF family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 467467UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639
PRO_0000108983

Regions

Nucleotide binding121 – 1277ATP Potential

Sequences

Sequence LengthMass (Da)Tools
Q8FZP2 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 460E444D6D7849CA

FASTA46748,766
        10         20         30         40         50         60 
MIPITALKDK TVALFGLGGS GIATAKAIVA GGARIIAWDD NPDSVARAQS AGIATGDLRQ 

        70         80         90        100        110        120 
ADWSQFAVFV LSPGVPLTHP QPHWSVDLAR AAGVEIIGDV ELFVRERNHI APDCPFIAIT 

       130        140        150        160        170        180 
GTNGKSTTTA LIAHIIKATG RDMQLGGNIG TAILTLEPPC ADRFYVVECS SYQIDLAPSL 

       190        200        210        220        230        240 
NPTAGILLNL TPDHLDRHGS MENYAAIKER LVAASGTAII GIDDAYCQAI ADRLHGAGIR 

       250        260        270        280        290        300 
VVRISKEKHL DRGYFADGAK LLWAQDGEID EIASLEGIGS LRGAHNAQNA LAAIVACLSA 

       310        320        330        340        350        360 
GLSLEEIHAG LKSFPGLAHR MEQVGRRGKV LFVNDSKATN AEATAPALSS FPQNIYWIVG 

       370        380        390        400        410        420 
GVPKAGGINS LTAFFPRVAK AYLIGEAAAQ FAATLGGAVP FEISDTLAAA VAHAAGDAGN 

       430        440        450        460 
DAAPEPVVLL SPACASFDQF QNFEKRGDAF RDAVLALPGV MPMRGES 

« Hide

References

[1]"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts."
Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. expand/collapse author list , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.
[2]"Revised genome sequence of Brucella suis 1330."
Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.
J. Bacteriol. 193:6410-6410(2011) [PubMed: 22038969] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014291 Genomic DNA. Translation: AAN30346.1.
CP002997 Genomic DNA. Translation: AEM18762.1.
RefSeqNP_698431.1. NC_004310.3.

3D structure databases

ProteinModelPortalQ8FZP2.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1167115.
GenomeReviewsGene locus BR1433 in contig AE014291_GR.
KEGGbms:BR1433.
NMPDRfig|204722.1.peg.1388.
PATRIC17791225. VBIBruSui107850_1459.
TIGRBR1433.

Phylogenomic databases

HOGENOMHBG750024.
OMAACASWDM.
PhylomeDBQ8FZP2.
ProtClustDBPRK01390.

Enzyme and pathway databases

BioCycBSUI204722:BR_1433-MONOMER.

Family and domain databases

HAMAPMF_00639. MurD.
[Tree]
InterProIPR018109. Folylpolyglutamate_synth_CS.
IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR016040. NAD(P)-bd_dom.
IPR005762. UDP-N-AcMur-Glu_ligase.
[Graphical view]
Gene3DG3DSA:3.90.190.20. Mur_ligase_C. 1 hit.
G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK01925.
PANTHERPTHR23135:SF2. PTHR23135:SF2. 1 hit.
PfamPF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMSSF53244. Mur_ligase_C. 1 hit.
SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMsTIGR01087. MurD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMURD_BRUSU
AccessionPrimary (citable) accession number: Q8FZP2
Secondary accession number(s): G0KBJ3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: March 1, 2003
Last modified: January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Brucella suis

Brucella suis (strain 1330): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families