Q8FZ50 (MASZ_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate synthase G
EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	BR1648, BS1330_I1642

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	728 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity. HAMAP MF_00641
Subcellular location	Cytoplasm By similarity HAMAP MF_00641.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 728	728	Malate synthase G HAMAP MF_00641		PRO_0000166883
Sites
	Active site	345	1	Proton acceptor By similarity
	Active site	636	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FZ50 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: F95669D002A14EDE	FASTA	728	79,967
	10 20 30 40 50 60 MGSAEKRNYV EIEGLAVAPE LVEFLAKEAA PGTGVEPEKF WKGFAAIIRD LAPKNRALLA 70 80 90 100 110 120 KRDELQARID AWYKENRDKG YSQADYQQFL KDIGYLLPEG GAFSVSTTNV DPEITHIAGP 130 140 150 160 170 180 QLVVPVMNAR YALNAANARW GSLYDALYGT DAISEADGAE KGKGYNPKRG EKVIAWAKNF 190 200 210 220 230 240 LDESAPLSTG KWADVAGLAV NDGKLEIRLT DGSATTLKDE SQFKGYNGDA ASPTNVLLAK 250 260 270 280 290 300 HNMHVDIVIN ADHPIGKTDP AHIADVVLES AISTIQDCED SIAAVDAEDK VAVYRNWLGL 310 320 330 340 350 360 MNGKLEDTFE KNGKQMTRRL NGDRTYTAPD GSTLTLKGHS LMLVRNVGHL MTNPAILDAE 370 380 390 400 410 420 GNEVPEGIMD AAFTSLIALH DIGPNGRHMN SREGSVYIVK PKMHGPEEVA FANEIFTRTE 430 440 450 460 470 480 EMLGMKPNTL KIGIMDEERR TTVNLKEAIR AAKDRVVFIN TGFLDRTGDE IHTSMEAGPM 490 500 510 520 530 540 IRKGDMKQAA WIGAYEQWNV DIGLECGLSG HAQIGKGMWA MPDMMAAMLE QKIAHPKAGA 550 560 570 580 590 600 NTAWVPSPTA ATLHATHYHK IDVAAVQEKL KSRPRAKLDD ILSVPVAVRP NWTPDDIQHE 610 620 630 640 650 660 IDNNAQGILG YVVRWIDQGV GCSKVPDINN VGLMEDRATL RISAQHIANW LYHGVVSEAQ 670 680 690 700 710 720 VMETMKRMAA IVDKQNEGDP LYRPMAADFD KSIAFQAACD LVFKGREQPN GYTEPVLHRR RLELKQAS

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed: 22038969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN30550.1. CP002997 Genomic DNA. Translation: AEM18966.1.
RefSeq	NP_698635.1. NC_004310.3.
3D structure databases
ProteinModelPortal	Q8FZ50.
SMR	Q8FZ50. Positions 10-725.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1167340.
GenomeReviews	Gene locus BR1648 in contig AE014291_GR.
KEGG	bms:BR1648.
NMPDR	fig|204722.1.peg.1592.
PATRIC	17791682. VBIBruSui107850_1675.
TIGR	BR1648.
Phylogenomic databases
HOGENOM	HBG350005.
OMA	PKMHGPD.
PhylomeDB	Q8FZ50.
ProtClustDB	PRK02999.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_1648-MONOMER.
Family and domain databases
HAMAP	MF_00641. Malate_synth_G. [Tree]
InterPro	IPR011076. Malate_synth-like. IPR023310. Malate_synth_G_beta_sub_dom. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
Gene3D	G3DSA:2.170.170.11. Malate_synth_G_beta_sub_dom. 2 hits.
KO	K01638.
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
SUPFAM	SSF51645. Malat_synth_like. 1 hit.
TIGRFAMs	TIGR01345. Malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_BRUSU

Accession

Primary (citable) accession number: Q8FZ50
Secondary accession number(s): G0K6I2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program