Q8FYX8 (PGK_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoglycerate kinase
EC=2.7.2.3

Gene names

Name:	pgk
Ordered Locus Names:	BR1729, BS1330_I1723

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella suis

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP-Rule MF_00145
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00145.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	Phosphoglycerate kinase HAMAP-Rule MF_00145		PRO_0000145917
Regions
	Nucleotide binding	352 – 355	4	ATP By similarity
	Region	20 – 22	3	Substrate binding By similarity
	Region	58 – 61	4	Substrate binding By similarity
Sites
	Binding site	35	1	Substrate By similarity
	Binding site	117	1	Substrate By similarity
	Binding site	150	1	Substrate By similarity
	Binding site	200	1	ATP By similarity
	Binding site	322	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FYX8 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: CF00FD7FB8EBB62D	FASTA	395	41,737
	10 20 30 40 50 60 MFRTLDDANV QSKRVLVRID LNVPMANGEV TDLTRIERIV PTIAELSRKG AKVILLAHFG 70 80 90 100 110 120 RPKGVASDEN SLKHVVKPLS KVLDHSVHFA EDCIGDKAKA AVDALKDGDV LLLENTRFHK 130 140 150 160 170 180 GEEKNDPEFV QALAANGDLY VNDAFSAAHR AHASTEGLAH VLPAFAGRAM QAELEALEKG 190 200 210 220 230 240 LGNPARPVVA IVGGAKVSTK LDLLSNLIEK VDALVIGGGM ANTFLAAKGL DVGKSLCEHE 250 260 270 280 290 300 LASTAREIMA KAETTKCAII LPVDAVVGWH FAADTPHQTY GVDSVPGDAM ILDAGELSTD 310 320 330 340 350 360 LIASAIDDAA TLVWNGPLGA FELRPFDTAT VKVARHVAKR TKEGKLVSVG GGGDTVAALN 370 380 390 HAGVVDDFTY ISTAGGAFLE WMEGKPLPSV DVLKK

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN30628.1. CP002997 Genomic DNA. Translation: AEM19045.1.
RefSeq	NP_698713.1. NC_004310.3. YP_005616537.1. NC_017251.1.
3D structure databases
ProteinModelPortal	Q8FYX8.
ModBase	Search...
Protein-protein interaction databases
STRING	204722.BR1729.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN30628; AAN30628; BR1729. AEM19045; AEM19045; BS1330_I1723.
GeneID	1167422. 12137493.
KEGG	bms:BR1729. bsi:BS1330_I1723.
PATRIC	17791846. VBIBruSui107850_1756.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0126.
HOGENOM	HOG000227107.
KO	K00927.
OMA	KFGLADK.
ProtClustDB	PRK00073.
Enzyme and pathway databases
UniPathway	UPA00109; UER00185.
Family and domain databases
Gene3D	3.40.50.1260. 1 hit. 3.40.50.1270. 1 hit.
HAMAP	MF_00145. Phosphoglyc_kinase.
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
PANTHER	PTHR11406. PTHR11406. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PIRSF	PIRSF000724. Pgk. 1 hit.
PRINTS	PR00477. PHGLYCKINASE.
SUPFAM	SSF53748. PGK. 1 hit.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGK_BRUSU

Accession

Primary (citable) accession number: Q8FYX8
Secondary accession number(s): G0K6Z0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program