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Q8FYS0 (HYPRE_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-hydroxyproline epimerase

EC=5.1.1.8
Alternative name(s):
Hydroxyproline-2-epimerase
Short name=BsHyPRE
Gene names
Ordered Locus Names:BR1792, BS1330_I1786
OrganismBrucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]
Taxonomic identifier204722 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length333 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Allows intracellular utilization of 4-hydroxyproline, one of the major constituents of host collagen, by converting 4-hydroxy-L-proline to 4-hydroxy-D-proline, which can be further metabolized by intracellular 4-hydroxy-D-proline oxidases. Strong B-cell mitogen. Plays an important role in the regulation of intra- and extracellular amino acid pools, allowing the bacterium to profit from host precursors and enzymatic pathways.

Catalytic activity

Trans-4-hydroxy-L-proline = cis-4-hydroxy-D-proline. Ref.1

Enzyme regulation

Inhibited by iodoacetate, iodoacetamide and by high amounts (10mM) of pyrrole-2-carboxylic acid (PYC). Not inhibited by PYC at 1mM. Ref.1

Subunit structure

Homodimer By similarity.

Miscellaneous

This enzyme does not require pyridoxal phosphate (PLP) as a cofactor.

Sequence similarities

Belongs to the proline racemase family. 4-hydroxyproline epimerase subfamily.

Biophysicochemical properties

Kinetic parameters:

KM=7.6 mM for 4-hydroxy-L-proline Ref.1

KM=10.8 mM for 4-hydroxy-D-proline

Vmax=0.50 µM/sec/mg enzyme with L-proline as substrate (at 37 degrees Celsius)

Vmax=0.75 µM/sec/mg enzyme with D-proline as substrate (at 37 degrees Celsius)

Ontologies

Keywords
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_function4-hydroxyproline epimerase activity

Inferred from electronic annotation. Source: UniProtKB-EC

proline racemase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3333334-hydroxyproline epimerase
PRO_0000354032

Sites

Active site901Proton acceptor By similarity
Active site2531Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8FYS0 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 320E7AA563495FBD

FASTA33336,592
        10         20         30         40         50         60 
MARHSFFCVD GHTCGNPVRL VAGGGPNLNG STMMEKRAHF LAEYDWIRTG LMFEPRGHDM 

        70         80         90        100        110        120 
MSGSILYPPT RPDCDVAVLF IETSGCLPMC GHGTIGTVTM AIEQGLVTPK TPGKLNLDTP 

       130        140        150        160        170        180 
AGLVAIEYEQ DGQYVERVRL TNVPAFLYAE GLEVECPDLG PIKVDVAYGG NFYAIVEPQE 

       190        200        210        220        230        240 
NYTDMDDYSA LQLIAWSPVL RQRLNEKYKF QHPELPDINR LSHILWTGKP KHPQAHARNA 

       250        260        270        280        290        300 
VFYGDKAIDR SPCGTGTSAR MAQLAAKGKL KPGDEFIHES IIGSLFHGRV ERAAEVAGRP 

       310        320        330 
AIVPSIAGWA RMTGYNTIFI DDRDPFAHGF SVA 

« Hide

References

« Hide 'large scale' references
[1]"Molecular and structural discrimination of proline racemase and hydroxyproline-2-epimerase from nosocomial and bacterial pathogens."
Goytia M., Chamond N., Cosson A., Coatnoan N., Hermant D., Berneman A., Minoprio P.
PLoS ONE 2:E885-E885(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
Strain: 1330.
[2]"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts."
Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. expand/collapse author list , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.
[3]"Revised genome sequence of Brucella suis 1330."
Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.
J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EF495343 Genomic DNA. Translation: ABS82395.1.
AE014291 Genomic DNA. Translation: AAN30688.1.
CP002997 Genomic DNA. Translation: AEM19105.1.
RefSeqNP_698773.1. NC_004310.3.
YP_005616597.1. NC_017251.1.

3D structure databases

ProteinModelPortalQ8FYS0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING204722.BR1792.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN30688; AAN30688; BR1792.
AEM19105; AEM19105; BS1330_I1786.
GeneID1167485.
12137556.
KEGGbms:BR1792.
bsi:BS1330_I1786.
PATRIC17791972. VBIBruSui107850_1819.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3938.
HOGENOMHOG000084336.
KOK12658.
OMAKNFRDMA.
OrthoDBEOG6SNDV3.

Enzyme and pathway databases

BRENDA5.1.1.8. 8693.

Family and domain databases

InterProIPR008794. Pro_racemase_fam.
[Graphical view]
PfamPF05544. Pro_racemase. 1 hit.
[Graphical view]
PIRSFPIRSF029792. Pro_racemase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHYPRE_BRUSU
AccessionPrimary (citable) accession number: Q8FYS0
Secondary accession number(s): A8DEY2, G0K7C9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Brucella suis

Brucella suis (strain 1330): entries and gene names