Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

4-hydroxyproline 2-epimerase

Gene

BR1792

Organism
Brucella suis biovar 1 (strain 1330)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Allows intracellular utilization of 4-hydroxyproline, one of the major constituents of host collagen, by converting trans-4-hydroxy-L-proline (t4LHyp) to cis-4-hydroxy-D-proline (c4DHyp), which can be further metabolized by intracellular 4-hydroxy-D-proline oxidases. Strong B-cell mitogen. Plays an important role in the regulation of intra- and extracellular amino acid pools, allowing the bacterium to profit from host precursors and enzymatic pathways. Displays no proline racemase activity.2 Publications

Catalytic activityi

Trans-4-hydroxy-L-proline = cis-4-hydroxy-D-proline.2 Publications

Enzyme regulationi

Inhibited by iodoacetate, iodoacetamide and by high amounts (10mM) of pyrrole-2-carboxylic acid (PYC). Not inhibited by PYC at 1mM.1 Publication

Kineticsi

  1. KM=7.6 mM for 4-hydroxy-L-proline1 Publication
  2. KM=10.8 mM for 4-hydroxy-D-proline1 Publication
  1. Vmax=0.50 µM/sec/mg enzyme with 4-hydroxy-L-proline as substrate (at 37 degrees Celsius)1 Publication
  2. Vmax=0.75 µM/sec/mg enzyme with 4-hydroxy-D-proline as substrate (at 37 degrees Celsius)1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei90 – 901Proton acceptorBy similarity
Binding sitei223 – 2231SubstrateBy similarity
Binding sitei249 – 2491SubstrateBy similarity
Active sitei253 – 2531Proton donorBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BRENDAi5.1.1.8. 8693.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxyproline 2-epimerase1 Publication (EC:5.1.1.82 Publications)
Short name:
4Hyp 2-epimerase
Short name:
4HypE1 Publication
Alternative name(s):
Hydroxyproline-2-epimerase1 Publication
Short name:
BsHyPRE1 Publication
Gene namesi
Ordered Locus Names:BR1792, BS1330_I1786
OrganismiBrucella suis biovar 1 (strain 1330)
Taxonomic identifieri204722 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000007104 Componenti: Chromosome I UP000000824 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3333334-hydroxyproline 2-epimerasePRO_0000354032Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi204722.BR1792.

Structurei

3D structure databases

ProteinModelPortaliQ8FYS0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni91 – 922Substrate bindingBy similarity
Regioni254 – 2552Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the proline racemase family.Curated

Phylogenomic databases

eggNOGiCOG3938.
HOGENOMiHOG000084336.
KOiK12658.
OMAiCPCGLVT.
OrthoDBiEOG6SNDV3.

Family and domain databases

InterProiIPR008794. Pro_racemase_fam.
[Graphical view]
PfamiPF05544. Pro_racemase. 1 hit.
[Graphical view]
PIRSFiPIRSF029792. Pro_racemase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8FYS0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARHSFFCVD GHTCGNPVRL VAGGGPNLNG STMMEKRAHF LAEYDWIRTG
60 70 80 90 100
LMFEPRGHDM MSGSILYPPT RPDCDVAVLF IETSGCLPMC GHGTIGTVTM
110 120 130 140 150
AIEQGLVTPK TPGKLNLDTP AGLVAIEYEQ DGQYVERVRL TNVPAFLYAE
160 170 180 190 200
GLEVECPDLG PIKVDVAYGG NFYAIVEPQE NYTDMDDYSA LQLIAWSPVL
210 220 230 240 250
RQRLNEKYKF QHPELPDINR LSHILWTGKP KHPQAHARNA VFYGDKAIDR
260 270 280 290 300
SPCGTGTSAR MAQLAAKGKL KPGDEFIHES IIGSLFHGRV ERAAEVAGRP
310 320 330
AIVPSIAGWA RMTGYNTIFI DDRDPFAHGF SVA
Length:333
Mass (Da):36,592
Last modified:March 1, 2003 - v1
Checksum:i320E7AA563495FBD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF495343 Genomic DNA. Translation: ABS82395.1.
AE014291 Genomic DNA. Translation: AAN30688.1.
CP002997 Genomic DNA. Translation: AEM19105.1.
RefSeqiNP_698773.1. NC_004310.3.
WP_004690494.1. NZ_KN046804.1.
YP_005616597.1. NC_017251.1.

Genome annotation databases

EnsemblBacteriaiAAN30688; AAN30688; BR1792.
AEM19105; AEM19105; BS1330_I1786.
KEGGibms:BR1792.
bsi:BS1330_I1786.
PATRICi17791972. VBIBruSui107850_1819.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF495343 Genomic DNA. Translation: ABS82395.1.
AE014291 Genomic DNA. Translation: AAN30688.1.
CP002997 Genomic DNA. Translation: AEM19105.1.
RefSeqiNP_698773.1. NC_004310.3.
WP_004690494.1. NZ_KN046804.1.
YP_005616597.1. NC_017251.1.

3D structure databases

ProteinModelPortaliQ8FYS0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi204722.BR1792.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN30688; AAN30688; BR1792.
AEM19105; AEM19105; BS1330_I1786.
KEGGibms:BR1792.
bsi:BS1330_I1786.
PATRICi17791972. VBIBruSui107850_1819.

Phylogenomic databases

eggNOGiCOG3938.
HOGENOMiHOG000084336.
KOiK12658.
OMAiCPCGLVT.
OrthoDBiEOG6SNDV3.

Enzyme and pathway databases

BRENDAi5.1.1.8. 8693.

Family and domain databases

InterProiIPR008794. Pro_racemase_fam.
[Graphical view]
PfamiPF05544. Pro_racemase. 1 hit.
[Graphical view]
PIRSFiPIRSF029792. Pro_racemase. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular and structural discrimination of proline racemase and hydroxyproline-2-epimerase from nosocomial and bacterial pathogens."
    Goytia M., Chamond N., Cosson A., Coatnoan N., Hermant D., Berneman A., Minoprio P.
    PLoS ONE 2:E885-E885(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: 1330.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1330.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1330.
  4. "Prediction and characterization of enzymatic activities guided by sequence similarity and genome neighborhood networks."
    Zhao S., Sakai A., Zhang X., Vetting M.W., Kumar R., Hillerich B., San Francisco B., Solbiati J., Steves A., Brown S., Akiva E., Barber A., Seidel R.D., Babbitt P.C., Almo S.C., Gerlt J.A., Jacobson M.P.
    Elife 3:0-0(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.

Entry informationi

Entry namei4HYPE_BRUSU
AccessioniPrimary (citable) accession number: Q8FYS0
Secondary accession number(s): A8DEY2, G0K7C9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: March 1, 2003
Last modified: May 27, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This enzyme does not require pyridoxal phosphate (PLP) as a cofactor.

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella suis
    Brucella suis (strain 1330): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.