Q8FYQ3 (ACSA_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-coenzyme A synthetase
EC=6.2.1.1

Alternative name(s):
Acetate--CoA ligase
Acyl-activating enzyme

Gene names

Name:	acsA
Ordered Locus Names:	BR1811, BS1330_I1805

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	651 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity. HAMAP MF_01123
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 651	651	Acetyl-coenzyme A synthetase HAMAP MF_01123		PRO_0000208357
Sites
	Active site	517	1	By similarity
Amino acid modifications
	Modified residue	611	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FYQ3 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 7FB222A3EE6476CE	FASTA	651	72,731
	10 20 30 40 50 60 MSEKLYPVLP EAKKNTLIDN ETYLEWYEES VSDPDGFWAK HGRRIDWFKP FTKVKNTDFN 70 80 90 100 110 120 GDVTIKWYED GVTNVSYNCI DRHLKSRGDK VAIIWEGDNP YIDKKITYRE LYENVCRMAN 130 140 150 160 170 180 VLKKHGVKKG DRVTIYLPMI PEAAYAMLAC ARIGAVHSVV FAGFSPEALA GRIVDCESTF 190 200 210 220 230 240 VITADEGVRG GKPVALKENT DTAIDIAAKQ YVMVNKVLVV RRTGGKVSWG RGRDLWYHQE 250 260 270 280 290 300 VASVEPHCEP EPMNAEDPLF ILYTSGSTGK PKGVLHTTGG YLVYASMTHQ YVFDYHDGEI 310 320 330 340 350 360 YWCTADVGWV TGHSYIVYGP LANGATTLMF EGVPNFPDQG RFWEVVDKHH VNIFYTAPTA 370 380 390 400 410 420 LRALMGAGDE FVTRSSRSTL RLLGSVGEPI NPEAWEWYYN VVGDQKCPIV DTWWQTENGG 430 440 450 460 470 480 ILITPLPGAT DLKPGSATRP FFGVKPVLVD NEGNVQEGVA DGNLCISDSW PGQMRTVYGD 490 500 510 520 530 540 HKRFIETYFS TYKGMYFSGD GCRRDEDGYY WITGRVDDVL NISGHRLGTA EIESALVSHH 550 560 570 580 590 600 SVSEAAVVGY PHPIKGQGIY CYVTLMTGAD AQDPDELRKE LVQHVRKEIG PIATPDKIQF 610 620 630 640 650 APGLPKTRSG KIMRRILRKI AEDEFGALGD TSTLADPGVV DDLIENRQNK K

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed: 22038969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN30706.1. CP002997 Genomic DNA. Translation: AEM19123.1.
RefSeq	NP_698791.1. NC_004310.3.
3D structure databases
ProteinModelPortal	Q8FYQ3.
SMR	Q8FYQ3. Positions 17-648.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1167504.
GenomeReviews	Gene locus BR1811 in contig AE014291_GR.
KEGG	bms:BR1811.
PATRIC	17792012. VBIBruSui107850_1839.
TIGR	BR1811.
Phylogenomic databases
HOGENOM	HBG547964.
OMA	TRGTEES.
PhylomeDB	Q8FYQ3.
ProtClustDB	PRK00174.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_1811-MONOMER.
Family and domain databases
HAMAP	MF_01123. Ac_CoA_synth. [Tree]
InterPro	IPR011904. Ac_CoA_lig. IPR024597. Acyl-CoA_synth_DUF3448. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
KO	K01895.
PANTHER	PTHR24095:SF42. PTHR24095:SF42. 1 hit.
Pfam	PF00501. AMP-binding. 1 hit. PF11930. DUF3448. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA_BRUSU

Accession

Primary (citable) accession number: Q8FYQ3
Secondary accession number(s): G0K7E7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program