Reviewed, UniProtKB/Swiss-Prot Q8FYM3 (PROA_BRUSU)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	BR1843

Organism

Brucella suis [Complete proteome] [HAMAP]

Taxonomic identifier

29461 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_0000189707

Sequences

	Sequence		Length	Mass (Da)
	Q8FYM3-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 875A0863AC4F966D	FASTA	421	44,099
	10 20 30 40 50 60 MTKDIAQVMA EVGRKAKAAA APLSIATSEQ KNKALNAAAD AILEARADIL EANRLDLANA 70 80 90 100 110 120 EKNGMAASFV DRLTLNEARI DAIAEDIRAI ATLPDPVGEV IAEWDRPNGL HIERVRTPLG 130 140 150 160 170 180 VIGVIYESRP NVTADAGALC LKAGNAVILR GGSDSAHSSA AIHKALVKGL EAANLPADAI 190 200 210 220 230 240 QIVPVTDRAA VGEMLKGLGG AIDVIVPRGG KSLVARVQSE ARVPVFAHLE GICHLYIDKS 250 260 270 280 290 300 ADLDMARRIA LDAKMRRTGI CGAAETLLVD RAVASTHLAP ILGDLAAGGC EIRGSAEVLA 310 320 330 340 350 360 LYPAAKPATE EDWSTEYLDA IISVALVDGI SGAIDHINRY SSHHTEAIVA EDAQTVARFF 370 380 390 400 410 420 NEIDSAILLH NASTQFADGG EFGMGAEIGI ATGKMHARGP VGVEQLTSFK YRVRGSGQVR G

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014291 Genomic DNA. Translation: AAN30738.1.
RefSeq	NP_698823.1.
3D structure databases
HSSP	HSSP built from PDB template 1O20 based on UniProtKB Q9WYC9.
ModBase	Search...
Genome annotation databases
GeneID	1167536.
GenomeReviews	Gene locus BR1843 in contig AE014291_GR.
KEGG	bms:BR1843.
NMPDR	fig|204722.1.peg.1780.
TIGR	BR1843.
Phylogenomic databases
HOGENOM	Q8FYM3.
OMA	Q8FYM3. APIISIK.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_1843-MON.
BRENDA	1.2.1.41. 281610.
Family and domain databases
HAMAP	MF_00412. [Tree]
InterPro	IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR000965. Gglut_pp_reduct. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_BRUSU

Accession

Primary (citable) accession number: Q8FYM3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 7, 2003
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)