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Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

tsaD

Organism
Brucella suis biovar 1 (strain 1330)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi115 – 1151IronUniRule annotation
Metal bindingi119 – 1191IronUniRule annotation
Binding sitei170 – 1701SubstrateUniRule annotation
Binding sitei183 – 1831Substrate; via amide nitrogenUniRule annotation
Binding sitei283 – 2831SubstrateUniRule annotation
Metal bindingi311 – 3111IronUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
Gene namesi
Name:tsaDUniRule annotation
Synonyms:gcp
Ordered Locus Names:BR1888, BS1330_I1882
OrganismiBrucella suis biovar 1 (strain 1330)
Taxonomic identifieri204722 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000007104 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359tRNA N6-adenosine threonylcarbamoyltransferasePRO_0000303297Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ8FYI5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni137 – 1415Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000109570.
KOiK01409.
OMAiRDHVKRM.
OrthoDBiEOG6K402S.

Family and domain databases

HAMAPiMF_01445. TsaD.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/TsaD.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_TsaD_YgjD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8FYI5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVLGIETSC DETAAAIVER DDMGEGRILS NVVLSQIAEH EPYGGVVPEI
60 70 80 90 100
AARAHVEALD RLVDRALNDA GLKLYEVDAV AATAGPGLIG GLIVGLMTAK
110 120 130 140 150
ALAMAAQKPF YAVNHLEGHA LTARLTDGLP FPYLLLLVSG GHTQMVLVRG
160 170 180 190 200
IGDYERLGTT IDDALGEAFD KTAKLLGLPY PGGPAVERMA LQGDQKRFAL
210 220 230 240 250
PRPLKGEARL DFSFSGLKTA VRQTATELVP LTDQDVTDIC ASFQAAVADT
260 270 280 290 300
LSDRVGRSLE RFKTEFPDCA TPSLVVAGGV AANKTLRAAL ENLCTRHGFA
310 320 330 340 350
FIAPPLNLCT DNAAMIAWAG AERAATQAPD SLDIAPRSRW PLDEKSAPVF

GTGRRGAKA
Length:359
Mass (Da):37,982
Last modified:March 1, 2003 - v1
Checksum:i975482F5A77E98C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN30782.1.
CP002997 Genomic DNA. Translation: AEM19199.1.
PIRiAB3274.
RefSeqiWP_002964958.1. NZ_KN046804.1.

Genome annotation databases

EnsemblBacteriaiAAN30782; AAN30782; BR1888.
AEM19199; AEM19199; BS1330_I1882.
KEGGibms:BR1888.
bsi:BS1330_I1882.
PATRICi17792194. VBIBruSui107850_1922.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN30782.1.
CP002997 Genomic DNA. Translation: AEM19199.1.
PIRiAB3274.
RefSeqiWP_002964958.1. NZ_KN046804.1.

3D structure databases

ProteinModelPortaliQ8FYI5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN30782; AAN30782; BR1888.
AEM19199; AEM19199; BS1330_I1882.
KEGGibms:BR1888.
bsi:BS1330_I1882.
PATRICi17792194. VBIBruSui107850_1922.

Phylogenomic databases

HOGENOMiHOG000109570.
KOiK01409.
OMAiRDHVKRM.
OrthoDBiEOG6K402S.

Family and domain databases

HAMAPiMF_01445. TsaD.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/TsaD.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_TsaD_YgjD. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1330.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1330.

Entry informationi

Entry nameiTSAD_BRUSU
AccessioniPrimary (citable) accession number: Q8FYI5
Secondary accession number(s): G0K7V3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: March 1, 2003
Last modified: November 11, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella suis
    Brucella suis (strain 1330): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.