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Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

tsaD

Organism
Brucella suis biovar 1 (strain 1330)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N6-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi115IronUniRule annotation1
Metal bindingi119IronUniRule annotation1
Binding sitei170SubstrateUniRule annotation1
Binding sitei183Substrate; via amide nitrogenUniRule annotation1
Binding sitei283SubstrateUniRule annotation1
Metal bindingi311IronUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processtRNA processing
LigandIron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
Gene namesi
Name:tsaDUniRule annotation
Synonyms:gcp
Ordered Locus Names:BR1888, BS1330_I1882
OrganismiBrucella suis biovar 1 (strain 1330)
Taxonomic identifieri204722 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000007104 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003032971 – 359tRNA N6-adenosine threonylcarbamoyltransferaseAdd BLAST359

Structurei

3D structure databases

ProteinModelPortaliQ8FYI5
SMRiQ8FYI5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni137 – 141Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000109570
KOiK01409
OMAiHLEGHIY

Family and domain databases

HAMAPiMF_01445 TsaD, 1 hit
InterProiView protein in InterPro
IPR000905 Gcp-like_dom
IPR017861 KAE1/TsaD
IPR022450 TsaD
PANTHERiPTHR11735 PTHR11735, 1 hit
PTHR11735:SF6 PTHR11735:SF6, 1 hit
PfamiView protein in Pfam
PF00814 Peptidase_M22, 1 hit
PRINTSiPR00789 OSIALOPTASE
TIGRFAMsiTIGR00329 gcp_kae1, 1 hit
TIGR03723 T6A_TsaD_YgjD, 1 hit

Sequencei

Sequence statusi: Complete.

Q8FYI5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVLGIETSC DETAAAIVER DDMGEGRILS NVVLSQIAEH EPYGGVVPEI
60 70 80 90 100
AARAHVEALD RLVDRALNDA GLKLYEVDAV AATAGPGLIG GLIVGLMTAK
110 120 130 140 150
ALAMAAQKPF YAVNHLEGHA LTARLTDGLP FPYLLLLVSG GHTQMVLVRG
160 170 180 190 200
IGDYERLGTT IDDALGEAFD KTAKLLGLPY PGGPAVERMA LQGDQKRFAL
210 220 230 240 250
PRPLKGEARL DFSFSGLKTA VRQTATELVP LTDQDVTDIC ASFQAAVADT
260 270 280 290 300
LSDRVGRSLE RFKTEFPDCA TPSLVVAGGV AANKTLRAAL ENLCTRHGFA
310 320 330 340 350
FIAPPLNLCT DNAAMIAWAG AERAATQAPD SLDIAPRSRW PLDEKSAPVF

GTGRRGAKA
Length:359
Mass (Da):37,982
Last modified:March 1, 2003 - v1
Checksum:i975482F5A77E98C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA Translation: AAN30782.1
CP002997 Genomic DNA Translation: AEM19199.1
PIRiAB3274
RefSeqiWP_002964958.1, NZ_KN046804.1

Genome annotation databases

EnsemblBacteriaiAAN30782; AAN30782; BR1888
AEM19199; AEM19199; BS1330_I1882
GeneIDi29594461
KEGGibms:BR1888
bsi:BS1330_I1882
PATRICifig|204722.21.peg.2523

Similar proteinsi

Entry informationi

Entry nameiTSAD_BRUSU
AccessioniPrimary (citable) accession number: Q8FYI5
Secondary accession number(s): G0K7V3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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