Q8FY59 (AROK_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Shikimate kinase
Short name=SK
EC=2.7.1.71

Gene names

Name:	aroK
Ordered Locus Names:	BR2029, BS1330_I2023

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella suis

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate By similarity. HAMAP-Rule MF_00109
Catalytic activity	ATP + shikimate = ADP + shikimate 3-phosphate. HAMAP-Rule MF_00109
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7. HAMAP-Rule MF_00109
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the shikimate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP chorismate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP shikimate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 200	200	Shikimate kinase HAMAP-Rule MF_00109		PRO_0000237856
Regions
	Nucleotide binding	33 – 38	6	ATP By similarity
Sites
	Metal binding	37	1	Magnesium By similarity
	Binding site	55	1	Substrate By similarity
	Binding site	79	1	Substrate By similarity
	Binding site	101	1	Substrate; via amide nitrogen By similarity
	Binding site	139	1	ATP By similarity
	Binding site	158	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FY59 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: F11D503663A80C62	FASTA	200	22,235
	10 20 30 40 50 60 MSGTNKQTNL HRQTETIRQL LGSKVVVLVG LMGAGKSTIG RKVANMLNLP FKDADTEIET 70 80 90 100 110 120 VSRMTVAELF EAYGEVEFRD LERRVILRLL DDGPMVLATG GGAYMNAETR AAIAEAGISI 130 140 150 160 170 180 WINADLDVLM ERVSRRQNRP LLRNSDPRGV MQRLMDERYP VYALAELHLM TRDEKKEVIA 190 200 AELIEVLAAH LEKEQAASAG

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN30919.1. CP002997 Genomic DNA. Translation: AEM19336.1.
RefSeq	NP_699004.1. NC_004310.3. YP_005616828.1. NC_017251.1.
3D structure databases
ProteinModelPortal	Q8FY59.
ModBase	Search...
Protein-protein interaction databases
STRING	204722.BR2029.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN30919; AAN30919; BR2029. AEM19336; AEM19336; BS1330_I2023.
GeneID	1167731. 12137811.
KEGG	bms:BR2029. bsi:BS1330_I2023.
PATRIC	17792485. VBIBruSui107850_2064.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0703.
HOGENOM	HOG000032568.
KO	K00891.
OMA	ADIPWIF.
ProtClustDB	PRK13946.
Enzyme and pathway databases
UniPathway	UPA00053; UER00088.
Family and domain databases
HAMAP	MF_00109. Shikimate_kinase.
InterPro	IPR000623. Shikimate_kinase. [Graphical view]
Pfam	PF01202. SKI. 1 hit. [Graphical view]
PRINTS	PR01100. SHIKIMTKNASE.
PROSITE	PS01128. SHIKIMATE_KINASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROK_BRUSU

Accession

Primary (citable) accession number: Q8FY59
Secondary accession number(s): G0K8P9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program