Reviewed, UniProtKB/Swiss-Prot Q8FY24 (DCUP_BRUSU)

Names and origin

Protein names

Recommended name:
    Uroporphyrinogen decarboxylase
      Short name=URO-D
      Short name=UPD
    EC=4.1.1.37

Gene names

Name:	hemE
Ordered Locus Names:	BR2066

Organism

Brucella suis [Complete proteome] [HAMAP]

Taxonomic identifier

29461 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III By similarity. HAMAP MF_00218
Catalytic activity	Uroporphyrinogen III = coproporphyrinogen + 4 CO2. HAMAP MF_00218
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4. HAMAP MF_00218
Subunit structure	Homodimer By similarity. HAMAP MF_00218
Subcellular location	Cytoplasm Probable HAMAP MF_00218.
Sequence similarities	Belongs to the uroporphyrinogen decarboxylase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	porphyrin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	uroporphyrinogen decarboxylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Uroporphyrinogen decarboxylase HAMAP MF_00218		PRO_0000187590
Regions
	Region	23 – 27	5	Substrate binding By similarity
Sites
	Binding site	42	1	Substrate By similarity
	Binding site	73	1	Substrate By similarity
	Binding site	148	1	Substrate By similarity
	Binding site	203	1	Substrate By similarity
	Binding site	318	1	Substrate By similarity
	Site	73	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FY24-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: DA4873C2CA945A82	FASTA	341	37,831
	10 20 30 40 50 60 MNRKVLKVID GETVFPPPIW MMRQAGRYLP EYRETRKKAG SFLDLCYSPD LAVEVTLQPI 70 80 90 100 110 120 RRFGFDAAIL FSDILVVPHA LGRDLRFEEG KGPLMTPIDA DEIFWLETEG VAKRLEPVYE 130 140 150 160 170 180 TVRLVREQLP DETTLLGFCG APWTVATYMI AGHGTPDQAP ARLFAYRFPE AFEKLLNDLA 190 200 210 220 230 240 DVSAEYLIEQ LGAGADAVQI FDSWSGVLDE DCFERFCIRP VARIVQKVRA VYPQARIIGF 250 260 270 280 290 300 PKGAGMLYAG YREKTGVDML GLDWSVPLSF AALLQEEGAV QGNLDPLRVV AGGNALDEGV 310 320 330 340 DAILERMGQG PLVFNLGHGI TPQAPIENVQ RMIDRVRGGK S

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330 / Biovar 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN30956.1.
RefSeq	NP_699041.1.
3D structure databases
SMR	Q8FY24. Positions 2-338.
ModBase	Search...
Genome annotation databases
GeneID	1167769.
GenomeReviews	Gene locus BR2066 in contig AE014291_GR.
KEGG	bms:BR2066.
TIGR	BR2066.
Phylogenomic databases
HOGENOM	HBG628392.
OMA	QIFDTWG.
PhylomeDB	Q8FY24.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_2066-MONOMER.
BRENDA	4.1.1.37. 281610.
Family and domain databases
HAMAP	MF_00218. URO-D. [Tree]
InterPro	IPR006361. Uroporphyrinogen_deCO2ase_HemE. IPR000257. Uroporphyrinogen_deCOase. [Graphical view]
PANTHER	PTHR21091:SF2. HemE. 1 hit.
Pfam	PF01208. URO-D. 1 hit. [Graphical view]
TIGRFAMs	TIGR01464. hemE. 1 hit.
PROSITE	PS00906. UROD_1. 1 hit. PS00907. UROD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DCUP_BRUSU

Accession

Primary (citable) accession number: Q8FY24

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 7, 2003
Last sequence update:	March 1, 2003
Last modified:	February 9, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)