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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Brucella suis biovar 1 (strain 1330)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691ShikimateUniRule annotation
Active sitei73 – 731Proton acceptorUniRule annotation
Binding sitei85 – 851NADPUniRule annotation
Binding sitei94 – 941ShikimateUniRule annotation
Binding sitei109 – 1091ShikimateUniRule annotation
Binding sitei226 – 2261NADP; via carbonyl oxygenUniRule annotation
Binding sitei228 – 2281ShikimateUniRule annotation
Binding sitei249 – 2491NADP; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi134 – 1385NADPUniRule annotation
Nucleotide bindingi158 – 1636NADPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00053; UER00087.

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:BR2069, BS1330_I2063
OrganismiBrucella suis biovar 1 (strain 1330)
Taxonomic identifieri204722 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000007104 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 289289Shikimate dehydrogenase (NADP(+))PRO_0000135994Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ8FY21.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 243Shikimate bindingUniRule annotation

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000237876.
KOiK00014.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00507. aroE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8FY21-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDKSMARGR KAFVTGFPIK HSRSPLIHGF WLKELGIDGS YEAVEVKPED
60 70 80 90 100
FSSFAASLAA NGFAGGNVTI PHKEAAYAAA ESLDEAACAI GAVNTLWLEN
110 120 130 140 150
GRLCGGNTDA YGFAANLDAS APGWDKADRA LVLGAGGASR AVVHALLSRG
160 170 180 190 200
VCHVSVVNRT LSRAEELAAH FGARVYAHGW DEAQALVSNA GLIVNTTALG
210 220 230 240 250
MSGHGEGQDF PIDLTCAPKE AVATNIVYVP LRTAFLNKAE KAGLKTVDGL
260 270 280
GMLLHQAVPG FERWFGQRPQ VTQALREHIL ADMAKAGAL
Length:289
Mass (Da):30,407
Last modified:November 7, 2003 - v2
Checksum:iBE867B20C895E43B
GO

Sequence cautioni

The sequence AAN30959 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AEM19376 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN30959.1. Different initiation.
CP002997 Genomic DNA. Translation: AEM19376.1. Different initiation.
RefSeqiWP_016769383.1. NZ_KN046804.1.

Genome annotation databases

EnsemblBacteriaiAAN30959; AAN30959; BR2069.
AEM19376; AEM19376; BS1330_I2063.
KEGGibms:BR2069.
bsi:BS1330_I2063.
PATRICi17792567. VBIBruSui107850_2104.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN30959.1. Different initiation.
CP002997 Genomic DNA. Translation: AEM19376.1. Different initiation.
RefSeqiWP_016769383.1. NZ_KN046804.1.

3D structure databases

ProteinModelPortaliQ8FY21.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN30959; AAN30959; BR2069.
AEM19376; AEM19376; BS1330_I2063.
KEGGibms:BR2069.
bsi:BS1330_I2063.
PATRICi17792567. VBIBruSui107850_2104.

Phylogenomic databases

HOGENOMiHOG000237876.
KOiK00014.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00087.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00507. aroE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAROE_BRUSU
AccessioniPrimary (citable) accession number: Q8FY21
Secondary accession number(s): G0K917
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: November 7, 2003
Last modified: September 7, 2016
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella suis
    Brucella suis (strain 1330): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.