Q8FXY4 (TRPB_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan synthase beta chain
EC=4.2.1.20

Gene names

Name:	trpB
Ordered Locus Names:	BR2110, BS1330_I2104

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella suis

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine By similarity. HAMAP-Rule MF_00133
Catalytic activity	L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + glyceraldehyde 3-phosphate + H2O. HAMAP-Rule MF_00133
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5. HAMAP-Rule MF_00133
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Sequence similarities	Belongs to the TrpB family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular_function	tryptophan synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 406	406	Tryptophan synthase beta chain HAMAP-Rule MF_00133		PRO_0000098925
Amino acid modifications
	Modified residue	99	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FXY4 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 5DC87B86C3CDFFA1	FASTA	406	43,571
	10 20 30 40 50 60 MNKPVAPNSY KTGPDEEGMF GIFGGRFVAE TLMPLILELQ QAYETAKNDP EFKAELNALS 70 80 90 100 110 120 TFYAGRPSKL YYAEGLSKHL GGAKIYFKRE DLNHTGSHKI NNCLGQILLA KRMGKTRIIA 130 140 150 160 170 180 ETGAGQHGVA SATVAARFGL PCIVYMGATD VERQKPNVFR MKLLGAEVKP VSAGNGTLKD 190 200 210 220 230 240 AMNEALRDWV TNVEDTYYLI GTAAGPHPYP ELVRDFQSVI GTEARQQILE QEGRLPDVIV 250 260 270 280 290 300 AAVGGGSNAI GLFHPFLDDA SVKIVGVEAG GRGLEGEEHC ASMSAGRPGV LHGNRTYLLQ 310 320 330 340 350 360 NADGQILEGH SVSAGLDYPG VGPEHSWLKD SGRVDYVPIL DNEALDAFQL CTRTEGIIPA 370 380 390 400 LESAHAIAQA VKMAPTMGKD KVMIVNLSGR GDKDVHTVGK LLGMDI

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN31000.1. CP002997 Genomic DNA. Translation: AEM19417.1.
RefSeq	NP_699085.1. NC_004310.3. YP_005616909.1. NC_017251.1.
3D structure databases
ProteinModelPortal	Q8FXY4.
SMR	Q8FXY4. Positions 20-403.
ModBase	Search...
Protein-protein interaction databases
STRING	204722.BR2110.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN31000; AAN31000; BR2110. AEM19417; AEM19417; BS1330_I2104.
GeneID	1167813. 12137893.
KEGG	bms:BR2110. bsi:BS1330_I2104.
PATRIC	17792653. VBIBruSui107850_2147.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0133.
HOGENOM	HOG000161710.
KO	K01696.
OMA	REDLCHT.
ProtClustDB	PRK04346.
Enzyme and pathway databases
UniPathway	UPA00035; UER00044.
Family and domain databases
HAMAP	MF_00133. Trp_synth_beta.
InterPro	IPR001926. Trp_syn_b_sub_like_PLP_eny_SF. IPR006653. Trp_synth_b_CS. IPR006654. Trp_synth_beta. IPR023026. Trp_synth_beta/beta-like. [Graphical view]
PANTHER	PTHR10314:SF3. PTHR10314:SF3. 1 hit.
Pfam	PF00291. PALP. 1 hit. [Graphical view]
PIRSF	PIRSF001413. Trp_syn_beta. 1 hit.
SUPFAM	SSF53686. PyrdxlP-dep_enz_bsu. 1 hit.
TIGRFAMs	TIGR00263. trpB. 1 hit.
PROSITE	PS00168. TRP_SYNTHASE_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPB_BRUSU

Accession

Primary (citable) accession number: Q8FXY4
Secondary accession number(s): G0K958

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program