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Q8FXX4

- SYFB_BRUSU

UniProt

Q8FXX4 - SYFB_BRUSU

Protein

Phenylalanine--tRNA ligase beta subunit

Gene

pheT

Organism
Brucella suis biovar 1 (strain 1330)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).UniRule annotation

    Cofactori

    Binds 2 magnesium ions per tetramer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi454 – 4541MagnesiumUniRule annotation
    Metal bindingi460 – 4601Magnesium; via carbonyl oxygenUniRule annotation
    Metal bindingi463 – 4631MagnesiumUniRule annotation
    Metal bindingi464 – 4641MagnesiumUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. phenylalanine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. phenylalanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
    2. tRNA processing Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phenylalanine--tRNA ligase beta subunitUniRule annotation (EC:6.1.1.20UniRule annotation)
    Alternative name(s):
    Phenylalanyl-tRNA synthetase beta subunitUniRule annotation
    Short name:
    PheRSUniRule annotation
    Gene namesi
    Name:pheTUniRule annotation
    Ordered Locus Names:BR2123, BS1330_I2117
    OrganismiBrucella suis biovar 1 (strain 1330)
    Taxonomic identifieri204722 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000000824: Chromosome I, UP000007104: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 804804Phenylalanine--tRNA ligase beta subunitPRO_0000126855Add
    BLAST

    Interactioni

    Subunit structurei

    Tetramer of two alpha and two beta subunits.UniRule annotation

    Protein-protein interaction databases

    STRINGi204722.BR2123.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8FXX4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini38 – 148111tRNA-bindingUniRule annotationAdd
    BLAST
    Domaini401 – 47676B5UniRule annotationAdd
    BLAST
    Domaini710 – 80394FDX-ACBUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.UniRule annotation
    Contains 1 B5 domain.UniRule annotation
    Contains 1 FDX-ACB domain.UniRule annotation
    Contains 1 tRNA-binding domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0073.
    HOGENOMiHOG000292085.
    KOiK01890.
    OMAiRTSCDET.
    OrthoDBiEOG6CCH1J.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.30.56.20. 1 hit.
    3.30.70.380. 1 hit.
    3.50.40.10. 1 hit.
    HAMAPiMF_00283. Phe_tRNA_synth_beta1.
    InterProiIPR005146. B3/B4_tRNA-bd.
    IPR009061. DNA-bd_dom_put.
    IPR012340. NA-bd_OB-fold.
    IPR004532. Phe-tRNA-ligase_IIc_bsu.
    IPR020825. Phe-tRNA_synthase_B3/B4.
    IPR005121. PheS_beta_Fdx_antiC-bd.
    IPR002547. tRNA-bd_dom.
    IPR005147. tRNA_synthase_B5-dom.
    [Graphical view]
    PfamiPF03483. B3_4. 1 hit.
    PF03484. B5. 1 hit.
    PF03147. FDX-ACB. 1 hit.
    PF01588. tRNA_bind. 1 hit.
    [Graphical view]
    SMARTiSM00873. B3_4. 1 hit.
    SM00874. B5. 1 hit.
    SM00896. FDX-ACB. 1 hit.
    [Graphical view]
    SUPFAMiSSF46955. SSF46955. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54991. SSF54991. 1 hit.
    SSF56037. SSF56037. 1 hit.
    TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
    PROSITEiPS51483. B5. 1 hit.
    PS51447. FDX_ACB. 1 hit.
    PS50886. TRBD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8FXX4-1 [UniParc]FASTAAdd to Basket

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    MKFTLSWLKD HLETDATLDE IVEKLTDIGL EVESVDDRAA FRAFTIARVL    50
    TATRHPDADK LQVLSVDTGD GKPVQVVCGA PNARAGLVGV LGRPGDYVPG 100
    LDVTLSVGKI RGVESFGMMC SERELELSDE HNGIIDLAEN APVGTSFAAY 150
    MGLDDPIIEI GLTPNRADCT GIRGIARDLA AAGLGTLKNT LPDAVKGEGE 200
    TPVKVILDQD AGNPFCTGFA LRMVKGVKNG PSPKWMQQRL KAIGLRPINA 250
    LVDITNYVTF DQGRPLHVFD AAKVKGNLTV RTARDGETIL ALDQREYKLN 300
    AGMYVIADEN GPESIAGIMG GEHSGCDENT VDVLIESALW DPRMIARTGR 350
    ELGIVTDARY RFERGVDPEM MVPGAEIATK LVLELCGGQP TVLDVVGYKP 400
    HTARVIDFPV TEVKRLTGLD VSYEDAFDIL KRLGFGVEGD GKTIRATVPS 450
    WRGDVEGKAD LVEEVMRIHG INRIDPQPLP SHGAVNGRIL TTLQIRTRHA 500
    RRMLASRGMM EAVTYSFISE AQAKAFGGGK PELKLANPIA ADMSDMRPSL 550
    LPGLLAAAQR NADRGFGDIA LFEVSGIYEG DTPDKQRRVA GGVRRGTAKV 600
    EGAGRFWAGN AAPVGVFDAK ADALAALEAA GAPVDRIQIE AGGPEWLHPG 650
    RSGTLKLGPK VVLGTFGEFH PDTLEALDVS GALCGFEVYL DAIPEPKAKS 700
    ARTKPALSLS LFQSLKRDYA FVVDAAVEAG NVVKAVSSAD KKLIVGVQVF 750
    DIFTGASLGE GKKSIAVEVL LQPQDRTLTD EDLEALSKQI VASVAKQTGG 800
    VLRG 804
    Length:804
    Mass (Da):85,849
    Last modified:March 1, 2003 - v1
    Checksum:i66E5F7F8268E875D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014291 Genomic DNA. Translation: AAN31013.1.
    CP002997 Genomic DNA. Translation: AEM19430.1.
    RefSeqiNP_699098.1. NC_004310.3.
    YP_005616922.1. NC_017251.1.

    Genome annotation databases

    EnsemblBacteriaiAAN31013; AAN31013; BR2123.
    AEM19430; AEM19430; BS1330_I2117.
    GeneIDi1167826.
    12137906.
    KEGGibms:BR2123.
    bsi:BS1330_I2117.
    PATRICi17792681. VBIBruSui107850_2161.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014291 Genomic DNA. Translation: AAN31013.1 .
    CP002997 Genomic DNA. Translation: AEM19430.1 .
    RefSeqi NP_699098.1. NC_004310.3.
    YP_005616922.1. NC_017251.1.

    3D structure databases

    ProteinModelPortali Q8FXX4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 204722.BR2123.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN31013 ; AAN31013 ; BR2123 .
    AEM19430 ; AEM19430 ; BS1330_I2117 .
    GeneIDi 1167826.
    12137906.
    KEGGi bms:BR2123.
    bsi:BS1330_I2117.
    PATRICi 17792681. VBIBruSui107850_2161.

    Phylogenomic databases

    eggNOGi COG0073.
    HOGENOMi HOG000292085.
    KOi K01890.
    OMAi RTSCDET.
    OrthoDBi EOG6CCH1J.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    3.30.56.20. 1 hit.
    3.30.70.380. 1 hit.
    3.50.40.10. 1 hit.
    HAMAPi MF_00283. Phe_tRNA_synth_beta1.
    InterProi IPR005146. B3/B4_tRNA-bd.
    IPR009061. DNA-bd_dom_put.
    IPR012340. NA-bd_OB-fold.
    IPR004532. Phe-tRNA-ligase_IIc_bsu.
    IPR020825. Phe-tRNA_synthase_B3/B4.
    IPR005121. PheS_beta_Fdx_antiC-bd.
    IPR002547. tRNA-bd_dom.
    IPR005147. tRNA_synthase_B5-dom.
    [Graphical view ]
    Pfami PF03483. B3_4. 1 hit.
    PF03484. B5. 1 hit.
    PF03147. FDX-ACB. 1 hit.
    PF01588. tRNA_bind. 1 hit.
    [Graphical view ]
    SMARTi SM00873. B3_4. 1 hit.
    SM00874. B5. 1 hit.
    SM00896. FDX-ACB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46955. SSF46955. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54991. SSF54991. 1 hit.
    SSF56037. SSF56037. 1 hit.
    TIGRFAMsi TIGR00472. pheT_bact. 1 hit.
    PROSITEi PS51483. B5. 1 hit.
    PS51447. FDX_ACB. 1 hit.
    PS50886. TRBD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1330.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1330.

    Entry informationi

    Entry nameiSYFB_BRUSU
    AccessioniPrimary (citable) accession number: Q8FXX4
    Secondary accession number(s): G0K971
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Brucella suis
      Brucella suis (strain 1330): entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3