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Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Brucella suis biovar 1 (strain 1330)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi148Zinc 1UniRule annotation1
Metal bindingi151Zinc 1UniRule annotation1
Metal bindingi165Zinc 2UniRule annotation1
Metal bindingi168Zinc 2UniRule annotation1
Metal bindingi187Zinc 2UniRule annotation1
Metal bindingi190Zinc 2UniRule annotation1
Metal bindingi201Zinc 1UniRule annotation1
Metal bindingi204Zinc 1UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri135 – 213CR-typeUniRule annotationAdd BLAST79

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

DNA replication, Stress response

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJUniRule annotation
Gene namesi
Name:dnaJUniRule annotation
Ordered Locus Names:BR2126, BS1330_I2120
OrganismiBrucella suis biovar 1 (strain 1330)
Taxonomic identifieri204722 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000007104 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000707441 – 377Chaperone protein DnaJAdd BLAST377

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ8FXX1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 69JUniRule annotationAdd BLAST66
Repeati148 – 155CXXCXGXG motif8
Repeati165 – 172CXXCXGXG motif8
Repeati187 – 194CXXCXGXG motif8
Repeati201 – 208CXXCXGXG motif8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi76 – 118Gly-richAdd BLAST43

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

Sequence similaritiesi

Belongs to the DnaJ family.UniRule annotation
Contains 1 CR-type zinc finger.UniRule annotation
Contains 1 J domain.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri135 – 213CR-typeUniRule annotationAdd BLAST79

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOGENOMiHOG000226717.
KOiK03686.
OMAiDEGMRIR.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsiTIGR02349. DnaJ_bact. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8FXX1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIDYYEALG VTRTADDKTL KAAFRKLAMQ YHPDRNPDDP EAERKFKEIG
60 70 80 90 100
EAYETLKDPQ KRAAYDRFGH AAFENGGMGG GFGNGFGGAG GFADIFEDIF
110 120 130 140 150
GEMMGGGRRR SNGGRERGAD LRYNMEVTLE EAYAGKTAQI RVPTSITCDE
160 170 180 190 200
CSGSGAKPGS QPTTCTMCSG SGRVRAAQGF FSVERTCPGC NGRGQIIKDP
210 220 230 240 250
CEKCHGQGRV TQERSLSVNI PAGIEDGTRI RLAGEGEAGL RGGPAGDLYI
260 270 280 290 300
FLSVKPHEFF QRDGADLYCK VPISMTTAAL GGQFEVSTLD GTQTRVKVPE
310 320 330 340 350
GTQNGKQFRL KGKGMPVLRQ SVTGDLYIQI DIETPQNLSK RQRELLEEFE
360 370
KLSSQENSPK SAGFFSRMKE FFEGIGE
Length:377
Mass (Da):41,078
Last modified:March 1, 2003 - v1
Checksum:iE66AA1F16606E775
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN31016.1.
CP002997 Genomic DNA. Translation: AEM19433.1.
PIRiAC3502.
RefSeqiWP_004684564.1. NZ_KN046804.1.

Genome annotation databases

EnsemblBacteriaiAAN31016; AAN31016; BR2126.
AEM19433; AEM19433; BS1330_I2120.
KEGGibms:BR2126.
bsi:BS1330_I2120.
PATRICi17792687. VBIBruSui107850_2164.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN31016.1.
CP002997 Genomic DNA. Translation: AEM19433.1.
PIRiAC3502.
RefSeqiWP_004684564.1. NZ_KN046804.1.

3D structure databases

ProteinModelPortaliQ8FXX1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN31016; AAN31016; BR2126.
AEM19433; AEM19433; BS1330_I2120.
KEGGibms:BR2126.
bsi:BS1330_I2120.
PATRICi17792687. VBIBruSui107850_2164.

Phylogenomic databases

HOGENOMiHOG000226717.
KOiK03686.
OMAiDEGMRIR.

Miscellaneous databases

PROiQ8FXX1.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsiTIGR02349. DnaJ_bact. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNAJ_BRUSU
AccessioniPrimary (citable) accession number: Q8FXX1
Secondary accession number(s): G0K974
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: March 1, 2003
Last modified: October 5, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella suis
    Brucella suis (strain 1330): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.