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Q8FXX1 (DNAJ_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chaperone protein DnaJ
Gene names
Name:dnaJ
Ordered Locus Names:BR2126, BS1330_I2120
OrganismBrucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]
Taxonomic identifier204722 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length377 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins By similarity. HAMAP-Rule MF_01152

Cofactor

Binds 2 zinc ions per monomer By similarity. HAMAP-Rule MF_01152

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01152

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01152.

Domain

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity By similarity. HAMAP-Rule MF_01152

Sequence similarities

Belongs to the DnaJ family.

Contains 1 CR-type zinc finger.

Contains 1 J domain.

Ontologies

Keywords
   Biological processDNA replication
Stress response
   Cellular componentCytoplasm
   DomainRepeat
Zinc-finger
   LigandMetal-binding
Zinc
   Molecular functionChaperone
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

protein folding

Inferred from electronic annotation. Source: InterPro

response to heat

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 377377Chaperone protein DnaJ HAMAP-Rule MF_01152
PRO_0000070744

Regions

Domain4 – 6966J
Repeat148 – 1558CXXCXGXG motif HAMAP-Rule MF_01152
Repeat165 – 1728CXXCXGXG motif HAMAP-Rule MF_01152
Repeat187 – 1948CXXCXGXG motif HAMAP-Rule MF_01152
Repeat201 – 2088CXXCXGXG motif HAMAP-Rule MF_01152
Zinc finger135 – 21379CR-type HAMAP-Rule MF_01152
Compositional bias76 – 11843Gly-rich HAMAP-Rule MF_01152

Sites

Metal binding1481Zinc 1 By similarity
Metal binding1511Zinc 1 By similarity
Metal binding1651Zinc 2 By similarity
Metal binding1681Zinc 2 By similarity
Metal binding1871Zinc 2 By similarity
Metal binding1901Zinc 2 By similarity
Metal binding2011Zinc 1 By similarity
Metal binding2041Zinc 1 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8FXX1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: E66AA1F16606E775

FASTA37741,078
        10         20         30         40         50         60 
MKIDYYEALG VTRTADDKTL KAAFRKLAMQ YHPDRNPDDP EAERKFKEIG EAYETLKDPQ 

        70         80         90        100        110        120 
KRAAYDRFGH AAFENGGMGG GFGNGFGGAG GFADIFEDIF GEMMGGGRRR SNGGRERGAD 

       130        140        150        160        170        180 
LRYNMEVTLE EAYAGKTAQI RVPTSITCDE CSGSGAKPGS QPTTCTMCSG SGRVRAAQGF 

       190        200        210        220        230        240 
FSVERTCPGC NGRGQIIKDP CEKCHGQGRV TQERSLSVNI PAGIEDGTRI RLAGEGEAGL 

       250        260        270        280        290        300 
RGGPAGDLYI FLSVKPHEFF QRDGADLYCK VPISMTTAAL GGQFEVSTLD GTQTRVKVPE 

       310        320        330        340        350        360 
GTQNGKQFRL KGKGMPVLRQ SVTGDLYIQI DIETPQNLSK RQRELLEEFE KLSSQENSPK 

       370 
SAGFFSRMKE FFEGIGE 

« Hide

References

[1]"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts."
Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. expand/collapse author list , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.
[2]"Revised genome sequence of Brucella suis 1330."
Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.
J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014291 Genomic DNA. Translation: AAN31016.1.
CP002997 Genomic DNA. Translation: AEM19433.1.
PIRAC3502.
RefSeqNP_699101.1. NC_004310.3.
YP_005616925.1. NC_017251.1.

3D structure databases

ProteinModelPortalQ8FXX1.
SMRQ8FXX1. Positions 2-77, 135-215.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING204722.BR2126.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN31016; AAN31016; BR2126.
AEM19433; AEM19433; BS1330_I2120.
GeneID1167829.
12137909.
KEGGbms:BR2126.
bsi:BS1330_I2120.
PATRIC17792687. VBIBruSui107850_2164.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0484.
HOGENOMHOG000226717.
KOK03686.
OMAGHTDPNQ.
OrthoDBEOG6BPDKP.

Family and domain databases

Gene3D1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPMF_01152. DnaJ.
InterProIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamPF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSPR00625. JDOMAIN.
SMARTSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsTIGR02349. DnaJ_bact. 1 hit.
PROSITEPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ8FXX1.

Entry information

Entry nameDNAJ_BRUSU
AccessionPrimary (citable) accession number: Q8FXX1
Secondary accession number(s): G0K974
Entry history
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Brucella suis

Brucella suis (strain 1330): entries and gene names