Q8FXW9 (PYRF_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Orotidine 5'-phosphate decarboxylase
EC=4.1.1.23

Alternative name(s):
OMP decarboxylase
Short name=OMPDCase
Short name=OMPdecase

Gene names

Name:	pyrF
Ordered Locus Names:	BR2128, BS1330_I2122

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella suis

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP) By similarity. HAMAP-Rule MF_01200
Catalytic activity	Orotidine 5'-phosphate = UMP + CO2. HAMAP-Rule MF_01200
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2. HAMAP-Rule MF_01200
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the OMP decarboxylase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' UMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway 'de novo' pyrimidine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	orotidine-5'-phosphate decarboxylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Orotidine 5'-phosphate decarboxylase HAMAP-Rule MF_01200		PRO_0000134531
Regions
	Region	67 – 76	10	Substrate binding By similarity
Sites
	Active site	69	1	Proton donor By similarity
	Binding site	18	1	Substrate By similarity
	Binding site	40	1	Substrate By similarity
	Binding site	122	1	Substrate By similarity
	Binding site	183	1	Substrate By similarity
	Binding site	192	1	Substrate By similarity
	Binding site	213	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FXW9 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: CBD7B7515095AD4B	FASTA	238	25,020
	10 20 30 40 50 60 MTTELHDDAS GRLIVGLDVP TIAEAEKVVE ELGNAVSFYK IGYQLVFAGG LDFAKSLVAA 70 80 90 100 110 120 RKKVFLDMKL LDIDNTIAKG VENVAKMGVS MLTLHAYPKA MRAAVEAARG SDLCLLGVTV 130 140 150 160 170 180 LTSMDNADLR EAGYSDNAET LVLKRARQAH EAGMGGIVAS AVEAQAIRQA VGPDMAIVTP 190 200 210 220 230 GIRPAGSEKG DQKRVMTPAD ALRAGASHLV VARPIVGAPD RKAAALAILK EMRSIGRS

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN31018.1. CP002997 Genomic DNA. Translation: AEM19435.1.
RefSeq	NP_699103.1. NC_004310.3. YP_005616927.1. NC_017251.1.
3D structure databases
ProteinModelPortal	Q8FXW9.
ModBase	Search...
Protein-protein interaction databases
STRING	204722.BR2128.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN31018; AAN31018; BR2128. AEM19435; AEM19435; BS1330_I2122.
GeneID	1167831. 12137911.
KEGG	bms:BR2128. bsi:BS1330_I2122.
PATRIC	17792691. VBIBruSui107850_2166.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0284.
HOGENOM	HOG000226070.
KO	K01591.
OMA	TVESSMR.
ProtClustDB	PRK00230.
Enzyme and pathway databases
UniPathway	UPA00070; UER00120.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01200_B. OMPdecase_type1_B.
InterPro	IPR013785. Aldolase_TIM. IPR014732. OMPdecase. IPR018089. OMPdecase_AS. IPR001754. OMPdeCOase_dom. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Pfam	PF00215. OMPdecase. 1 hit. [Graphical view]
SMART	SM00934. OMPdecase. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR01740. pyrF. 1 hit.
PROSITE	PS00156. OMPDECASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRF_BRUSU

Accession

Primary (citable) accession number: Q8FXW9
Secondary accession number(s): G0K976

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 23, 2003
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program