Q8FXT9 (LNT_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Apolipoprotein N-acyltransferase
Short name=ALP N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lnt
Ordered Locus Names:	BR2158, BS1330_I2152

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella suis

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers the fatty acyl group on membrane lipoproteins By similarity. HAMAP-Rule MF_01148
Pathway	Protein modification; lipoprotein biosynthesis (N-acyl transfer). HAMAP-Rule MF_01148
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_01148.
Sequence similarities	Belongs to the CN hydrolase family. Apolipoprotein N-acyltransferase subfamily. Contains 1 CN hydrolase domain.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	lipoprotein biosynthetic process Inferred from electronic annotation. Source: HAMAP nitrogen compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	N-acyltransferase activity Inferred from electronic annotation. Source: HAMAP hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 532	532	Apolipoprotein N-acyltransferase HAMAP-Rule MF_01148		PRO_0000178051
Regions
	Transmembrane	13 – 30	18	Helical; Potential
	Transmembrane	35 – 52	18	Helical; Potential
	Transmembrane	73 – 95	23	Helical; Potential
	Transmembrane	99 – 121	23	Helical; Potential
	Transmembrane	133 – 155	23	Helical; Potential
	Transmembrane	177 – 199	23	Helical; Potential
	Transmembrane	204 – 226	23	Helical; Potential
	Transmembrane	505 – 527	23	Helical; Potential
	Domain	240 – 532	293	CN hydrolase

Sequences

	Sequence		Length	Mass (Da)
	Q8FXT9 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: BE5FF9DE257148B5	FASTA	532	57,461
	10 20 30 40 50 60 MIARLAGRII LLSGWRRALA AFLSGAFATL TQPPFDIFVA GFVSFPVLVW LIDGAIARTD 70 80 90 100 110 120 AGPLRRLLPA AKVGWWFGFG YFVSGLWWIG TALLVDADQF AWALPLAVLG LPAFLALFYA 130 140 150 160 170 180 FAAMIARLLW SDGLGRILAL AFGFALAEWL RTFIFTGFPW NLIGYAAMPV PLLMQSVAVI 190 200 210 220 230 240 GLVGMSALAV FVFAAPALLT GGHFARTGIG LAIFLALAHV GFGAWTLSRA PAIVDENGPL 250 260 270 280 290 300 AVRIVQPSIA QAMKWDNAER RAIFDKLVGL TEEAPAEGKP RPDVIVWPET AIPYILESTP 310 320 330 340 350 360 QALAHIGDAL QEGQVLLAGA VREEKGADGG EPRYYNSIYT IDDRGRIVST ADKVHLVPFG 370 380 390 400 410 420 EYLPFESFLR GLGLQEVVEM PGGFTAGTTR HALAVKDGRS FLPLICYEAI FPDELGYEGA 430 440 450 460 470 480 GASAIINVTN DAWYGDTPGP YQHFRQAQVR AVEQGLPLIR AANNGLSAIV NTYGRITGSL 490 500 510 520 530 ALDAVGVVDS YLPSPRDPFW GRPPGWIQTV LILLTLLAAS VGLILYSRRR FH

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN31048.1. CP002997 Genomic DNA. Translation: AEM19465.1.
RefSeq	NP_699133.1. NC_004310.3. YP_005616957.1. NC_017251.1.
3D structure databases
ProteinModelPortal	Q8FXT9.
ModBase	Search...
Protein-protein interaction databases
STRING	204722.BR2158.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN31048; AAN31048; BR2158. AEM19465; AEM19465; BS1330_I2152.
GeneID	1167861. 12138581.
KEGG	bms:BR2158. bsi:BS1330_I2152.
PATRIC	17792749. VBIBruSui107850_2195.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0815.
HOGENOM	HOG000264280.
KO	K03820.
OMA	HGEIIAG.
ProtClustDB	PRK00302.
Enzyme and pathway databases
UniPathway	UPA00666.
Family and domain databases
Gene3D	3.60.110.10. 1 hit.
HAMAP	MF_01148. Lnt.
InterPro	IPR004563. Apolipo_AcylTrfase. IPR003010. C-N_Hydrolase. [Graphical view]
Pfam	PF00795. CN_hydrolase. 1 hit. [Graphical view]
SUPFAM	SSF56317. Ntlse/CNhydtse. 1 hit.
TIGRFAMs	TIGR00546. lnt. 1 hit.
PROSITE	PS50263. CN_HYDROLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LNT_BRUSU

Accession

Primary (citable) accession number: Q8FXT9
Secondary accession number(s): G0K9I4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program