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Q8FXT6 (TRMB_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:BR2161, BS1330_I2155
OrganismBrucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]
Taxonomic identifier204722 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length233 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 233233tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171306

Regions

Region212 – 2154Substrate binding By similarity

Sites

Active site1381 By similarity
Binding site641S-adenosyl-L-methionine By similarity
Binding site891S-adenosyl-L-methionine By similarity
Binding site1161S-adenosyl-L-methionine By similarity
Binding site1381S-adenosyl-L-methionine By similarity
Binding site1421Substrate By similarity
Binding site1741Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8FXT6 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 87BB319216B97E03

FASTA23327,024
        10         20         30         40         50         60 
MIDENHPMRA AGNFFGRRHG KPLRPHQSNL FEDLLPRLKL DLATPASQDL RSLFEAPVET 

        70         80         90        100        110        120 
VRMEIGFGGG EHLHHESGRY PQSGFIGVEP FINGMAKMLA ALDQAPRPNL RLYDEDATAV 

       130        140        150        160        170        180 
LDWLPDASLA GIDLFYPDPW HKRRHWKRRF VSDANLDRFA RVLKPGAKFR FASDIEHYVN 

       190        200        210        220        230 
WTLQHCRRHA AFDWQAESPA DWNDAYEGWP GTRYEAKAFR EGRRAVYLTF IRR 

« Hide

References

[1]"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts."
Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. expand/collapse author list , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.
[2]"Revised genome sequence of Brucella suis 1330."
Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.
J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014291 Genomic DNA. Translation: AAN31051.1.
CP002997 Genomic DNA. Translation: AEM19468.1.
RefSeqNP_699136.1. NC_004310.3.
YP_005616960.1. NC_017251.1.

3D structure databases

ProteinModelPortalQ8FXT6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING204722.BR2161.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN31051; AAN31051; BR2161.
AEM19468; AEM19468; BS1330_I2155.
GeneID1167864.
12138584.
KEGGbms:BR2161.
bsi:BS1330_I2155.
PATRIC17792755. VBIBruSui107850_2198.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073969.
KOK03439.
OMARAFYGRR.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_BRUSU
AccessionPrimary (citable) accession number: Q8FXT6
Secondary accession number(s): G0K9I7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: March 1, 2003
Last modified: June 11, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Brucella suis

Brucella suis (strain 1330): entries and gene names